Automated cell count in flow cytometry: A valuable tool to assess CD4 absolute levels in peripheral blood

American Journal of Clinical Pathology

Volumn 110, Issue 3, 1998, Pages 321-326

  Béné, Marie C ^a   Kolopp Sarda, Marie N ^a   El Kaissouni, Jamal ^a   De March Kennel, Anne ^a   Molé, Claire ^a   Kohler, Chantal ^a   Faure, Gilbert C ^a  

^a NONE

Author keywords

Automated count; Flow cytometry; HIV; Lymphocytes

Indexed keywords

CD4 ANTIGEN; MONOCLONAL ANTIBODY;

ARTICLE; AUTOANALYSIS; BLOOD SAMPLING; CELL COUNT; COMPUTER ANALYSIS; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; LEUKOCYTE COUNT; LYMPHOCYTE SUBPOPULATION; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; VIRUS INFECTION;

EID: 20644465611     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1093/ajcp/110.3.321     Document Type: Article

References (13)

1. Giorgi JV, Cheng HK, Margolick JB, et al. Quality control in flow cytometric measurement of T-lymphocyte subsets: the multicenter AIDS cohort study experience. Clin Immunol Immunopathol. 1990;55:173-186.
2. Nicholson JKA, Jones BM, Hubbard M. CD4 T-lymphocyte determination on whole blood specimens using a single tube three color assay. Cytometry. 1993;14:685-689.
3. Caldwell C, Taylor HM. A rapid no-wash technic for immunophenotypic analysis by flow cytometry. Am J Clin Pathol. 1986;86:600-607.
4. Renzi P, Ginns L. Analysis of T cell subsets in normal adults: comparison of whole blood lysis to Ficoll Hypaque separation by flow cytometry. J Immunol Methods. 1987;98:53-56.
5. Ashmore LM, Shopp GM, Edwards BS. Lymphocyte subset analysis by flow cytometry: comparison of three different staining techniques and effects of blood storage. J Immunol Methods. 1989;118:209-215.
6. Terstrappen LWMM, Meiners H, Loken MR. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989;123:103-112.
7. Mansour I, Bourin P, Rouger P, et al. A rapid technique for lymphocyte preparation prior to two-color immunofluorescence analysis of lymphocyte subsets using flow cytometry. J Immunol Methods. 1990;127:61-70.
8. + T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR Morbid Mortal Wkly Rep. 1997;46:1-29.
9. Mandy FF, Bergeron M, Recktenwald D, et al. A simultaneous three-color T-cell subsets analysis with single laser flow cytometers using T-cell gating protocol: comparison with conventional two-color immunophenotyping method. J Immunol Methods. 1992;156:151-162.
10. Calvelli T, Denny TN, Paxton H, et al. Guideline for flow cytometric immunophenotyping: a report from the National Institute of Allergy and Infectious Diseases, Division of AIDS. Cytometry. 1993;14:702-715.
11. Cohen AJ, Peerschke EIB, Steigbigel R. A comparison of the Coulter STKS, Coulter S+IV, and manual analysis of white blood cell differential counts in a human immunodeficiency virus-infected population. Am J Clin Pathol. 1993;100: 611-617.
12. Simson E, Groner W. Variability in absolute lymphocyte counts obtained by automated cell counters. Cytometry. 1995;22:26-34.
13. Bentley SA. Automated differential white cell counts: a critical appraisal. Baillieres Clin Haematol. 1990;3:851-869.