Application of real-time RT-PCR for large-scale testing of potato for Potato spindle tuber pospiviroid

EPPO Bulletin

Volumn 35, Issue 1, 2005, Pages 133-140

  Roenhorst, J W ^a   Jansen, C C C ^a   Kox, L F F ^a   De Haan, E G ^b   Van Den Bovenkamp, G W ^b   Boonham, N ^c   Fisher, T ^c   Mumford, R A ^c  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NAK   (Netherlands)

^c CENTRAL SCIENCE LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

POSPIVIROID; SOLANUM TUBEROSUM; VIROIDS;

EID: 20444428403     PISSN: 02508052     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2338.2005.00798.x     Document Type: Conference Paper

References (9)

1. Boonham N, Gonzáles Pérez L, Mendez MS, Lilia Peralta E, Blockley A, Walsh K, Barker I & Mumford RA (2004) Development of a real-time RT-PCR assay for the detection of Potato spindle tuber viroid. (Pstvd) Journal of Virological Methods 116, 139-146.
2. EPPO/CABI (1997) Potato spindle tuber viroid. In: 'Quarantine Pests for Europe', pp. 1305-1310. 2nd edn. CAB International, Wallingford (UK).
3. Harris PS & Browning IA (1980) The effects of temperature and light on the symptom expression and viroid concentration in tomato of a severe strain of potato spindle tuber viroid. Potato Research 23, 85-93.
4. Jeffries C & James C (2004) Development of an EU protocol for the detection and diagnosis of Potato spindle tuber pospiviroid. Bulletin OEPP/IEPPO Bulletin 35, 125-132.
5. Kox LFF, Boxman ILA, Jansen CCC & Roenhorst JW (2004) Reliability of nucleic acid amplification techniques. Modified target RNA as exogenous internal standard for a real-time RT-PCR for Potato spindle tuber pospiviroid. Bulletin OEPP/IEPPO Bulletin 35, 117-124.
6. Ollivier F (2004) L'hybridation moléculaire: une technique de détection du Potato spindle tuber viroid adaptée aux plans de surveillance. Bulletin OEPP/IEPPO Bulletin 34, 123-126.
7. Roenhorst JW, Bûtot RPT, van der Heijden KA, Hooftman M & van Zaayen A (2000) Detection of Chrysanthemum stunt viroid and Potato spindle tuber viroid by return-polyacrylamide gel electrophoresis. Bulletin OEPP/IEPPO Bulletin 30, 453-456.
8. Verhoeven J, ThJ, Jansen CCC, Willemen TM, Kox LFF, Owens RA & Roenhorst JW (2004) Natural infections of tomato by Citrus exocortis viroid, Columnea latent viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. European Journal of Plant Pathology 110, 823-831.
9. Weller SA, Elphinstone JG, Smith NC, Boonham N & Stead DE (2000) Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Applied and Environmental Microbiology 66, 2853-2858.