Characterization of Wolbachia transfection efficiency by using microinjection of embryonic cytoplasm and embryo homogenate

Applied and Environmental Microbiology

Volumn 71, Issue 6, 2005, Pages 3199-3204

  Xi, Zhiyong ^a   Dobson, Stephen L ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOSYNTHESIS; DNA; ENZYMES; GENES; MUTAGENS;

ARTIFICIAL TRANSFER; CYTOPLASMIC INCOMPATIBILITY; INVERTEBRATES; WOLBCHIA TRANSFECTION;

MICROBIOLOGY;

RINGER SOLUTION;

BACTERIUM;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; GENETIC TRANSFECTION; GERM LINE; HOMOGENATE; IN VITRO STUDY; MICROINJECTION; NONHUMAN; WOLBACHIA;

ANIMALS; BUFFERS; CYTOPLASM; DROSOPHILA; MICROINJECTIONS; WOLBACHIA;

ALPHAPROTEOBACTERIA; HEXAPODA; INSECTA; INVERTEBRATA; RICKETTSIA; WOLBACHIA;

EID: 20444427895     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.6.3199-3204.2005     Document Type: Article

References (22)

1. Ashburner, M. 1989. Drosophila, a laboratory handbook. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
2. Bovarnick, M. R., J. C. Miller, and J. C. Snyder. 1950. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J. Bacteriol. 59:509-522.
3. Boyle, L., S. L. O'Neill, H. M. Robertson, and T. L. Karr. 1993. Interspecific and intraspecific horizontal transfer of Wolbachia in Drosophila. Science 260:1796-1799.
4. Braig, H. R., H. Guzman, R. B. Tesh, and S. L. O'Neill. 1994. Replacement of the natural Wolbachia symbiont of Drosophila simulans with a mosquito counterpart. Nature 367:453-455.
5. Dobson, S. L. 2003. Reversing Wolbachia-based population replacement. Trends Parasitol. 19:128-133.
6. Dobson, S. L., E. J. Marsland, Z. Veneti, K. Bourtzis, and S. L. O'Neill. 2002. Characterization of Wolbachia host cell range via the in vitro establishment of infections. Appl. Environ. Microbiol. 68:656-660.
7. Driever, W., V. Siegel, and C. Nusslein-Volhard. 1990. Autonomous determination of anterior structures in the early Drosophila embryo by bicoid morphogen. Development 109:811-820.
8. Gilbert, S. F. 1997. Developmental biology, 5th ed. Sinauer Associates, Inc., Sunderland, Massachusetts.
9. Giordano, R., S. L. O'Neill, and H. M. Robertson. 1995. Wolbachia infections and the expression of cytoplasmic incompatibility in Drosophila sechellia and D. mauritiana. Genetics 140:1307-1317.
10. Hartmann, N., H. Stuckas, R. Lucius, W. Bleiss, F. Theuring, and B. H. Kalinna. 2003. Trans-species transfer of Wolbachia: microinjection of Wolbachia from Litomosoides sigmodontis into Acanthocheilonema viteae. Parasitology 126:503-511.
11. Heddi, A., A. M. Grenier, C. Khatchadourian, H. Charles, and P. Nardon. 1999. Four intracellular genomes direct weevil biology: nuclear, mitochondrial, principal endosymbiont, and Wolbachia. Proc. Natl. Acad. Sci. USA 96:6814-6819.
12. Illmensee, K., and A. P. Mahowald. 1974. Transplantation of posterior polar plasm in Drosophila. Induction of germ cells at the anterior pole of the egg. Proc. Natl. Acad. Sci. USA 71:1016-1020.
13. Lassy, C. W., and T. L. Karr. 1996. Cytological analysis of fertilization and early embryonic development in incompatible crosses of Drosophila simulans. Mech. Dev. 57:47-58.
14. Mahowald, A. P., K. Illmensee, and F. R. Turner. 1976. Interspecific transplantation of polar plasm between Drosophila embryos. J. Cell Biol. 70:358-373.
15. McGraw, E. A., D. J. Merritt, J. N. Droller, and S. L. O'Neill. 2002. Wolbachia density and virulence attenuation after transfer into a novel host. Proc. Natl. Acad. Sci. USA 99:2918-2923.
16. Poinsot, D., K. Bourtzis, G. Markakis, C. Savakis, and H. Mercot. 1998. Wolbachia transfer from Drosophila melanogaster into D. simulans: host effect and cytoplasmic incompatibility relationships. Genetics 150:227-237.
17. Riegler, M., S. Charlat, C. Stauffer, and H. Mercot. 2004. Wolbachia transfer from Rhagoletis cerasi to Drosophila simulans: investigating the outcomes of host-symbiont coevolution. Appl. Environ. Microbiol. 70:273-279.
18. Santamaria, P. 1986. Injecting eggs, p. 159-173. In D. B. Roberts (ed.), Drosophila, a practical approach. IRL Press Ltd., Oxford, United Kingdom.
19. Werren, J. H., and D. M. Windsor. 2000. Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proc. R. Soc. London Ser. B 267:1277-1285.
20. Werren, J. H., W. Zhang, and L. R. Guo. 1995. Evolution and phytogeny of Wolbachia: reproductive parasites of arthropods. Proc. R. Soc. London Ser. B 261:55-63.
21. Wu, M., L. V. Sun, J. Vamathevan, M. Riegler, R. Deboy, J. C. Brownlie, E. A. McGraw, W. Martin, C. Esser, N. Ahmadinejad, C. Wiegand, R. Madupu, M. J. Beanan, L. M. Brinkac, S. C. Daugherty, A. S. Durkin, J. F. Kolonay, W. C. Nelson, Y. Mohamoud, P. Lee, K. Berry, M. B. Young, T. Utterback, J. Weidman, W. C. Nierman, I. T. Paulsen, K. E. Nelson, H. Tettelin, S. L. O'Neill, and J. A. Eisen. 16 March 2004, posting date. Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. PLoS Biol. 2:E69. [Online.] http://biology.plosjournals.org.
22. Zhou, W., F. Roussel, and S. O'Neill. 1998. Phytogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc. R. Soc. London Ser. B 265:509-515.