The removal of pyroglutamic acid from monoclonal antibodies without denaturation of the protein chains

Analytical Biochemistry

Volumn 342, Issue 1, 2005, Pages 120-125

  Werner, William E ^a   Wu, Sylvia ^a   Mulkerrin, Michael ^a  

^a Abgenix   (United States)

Author keywords

Antibody analysis; Antibody sequencing; Blocked N terminal sequencing; Protein sequencing; Pyroglutamate removal

Indexed keywords

MONOCLONAL ANTIBODIES; SOAPS (DETERGENTS);

AMINO PEPTIDASE; EDMAN SEQUENCING; ELEVATED TEMPERATURE; N-TERMINAL SEQUENCING; PROTEIN SEQUENCING; PYROCOCCUS FURIOSUS; PYROGLUTAMIC ACIDS; SAMPLE CLEAN-UP;

REDUCING AGENTS;

DETERGENT; IMMUNOGLOBULIN HEAVY CHAIN; MONOCLONAL ANTIBODY; POLYSORBATE 20; PYROGLUTAMIC ACID;

ARTICLE; ARTIFICIAL MEMBRANE; ENZYME ISOLATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN STABILITY; PYROCOCCUS FURIOSUS; SEPARATION TECHNIQUE; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

PYROCOCCUS FURIOSUS;

EID: 20444425289     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2005.04.012     Document Type: Article

References (7)

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