Expression of cytosine deaminase gene in human colon carcinoma cells by recombinant retroviral vector

Acta Biochimica et Biophysica Sinica

Volumn 29, Issue 2, 1997, Pages 140-141

  Jiang, Qiong ^a   Ge, Kai ^a   Xu, De Hua ^a   Sun, Lan Ying ^a   Zheng, Zhong Cheng ^a   Liu, Xin Yuan ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

EC CD gene; Gene therapy; Human colon carcinoma cell

Indexed keywords

EID: 20444420926     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Mullen C A, Mogens Kilstrup, Blaese R M. Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: A negative selection system. Proc Natl Acad Sci USA, 1992, 89: 33-37.
2. Austin E A, Huber B E. A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase. Molecular pharmacology, 43: 380-387.
3. Huber B E, Austin E A, Good S S et al. In vivo antitumor activity of 5-fluorocytosine on human colorectal carcinoma cells genetically modified to express cytosine deaminase. Cancer Research, 1993, 53: 4619-4626.
4. Hitoshi Kotani, Perry B, Newton III et al. Improved methods of retroviral vector transduction and production for gene therapy. Hum Gene Ther, 1994, 5: 19-28.
5. Mullen C A, Coale M M, Robert lowe et al. Tumors expressing the cytosine deaminase suicide gene can be eliminated in vivo with 5-fluorocytosine and induce protective immunity to wild type tumor. Cancer Research, 1994, 54: 1503-1506.