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2+ influx J Neurosci 24 2004 1226 1235 In this study, calcium imaging is performed on isolated nerve terminals from hypothalamic neurons under whole-cell recording. RyRs are shown to be present at the terminals through immunocytochemistry. The study offers the first recordings of depolarization-dependent calcium release events (elegantly named Syntillas) in the absence of extracellular calcium. The results of modeling of the amplitude and spatial extent of the calcium changes induced by syntillas are consistent with a potential role for these events as triggers of neuropeptide release.
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A. Lelli, P. Perin, M. Martini, C.D. Ciubotaru, I. Prigioni, P. Valli, M.L. Rossi, and F. Mammano Presynaptic calcium stores modulate afferent release in vestibular hair cells J Neurosci 23 2003 6894 6903 The authors use thin slices of the frog semicircular canal to study hair cells under whole-cell recording. This study establishes the role of presynaptic calcium stores in afferent synapses (carrying sensory signals towards higher brain centers). The work helps establish a correlation between presynaptic calcium stores and transmitter release by showing that, in response to caffeine and depolarization, ryanodine-sensitive calcium rises are accompanied by changes in membrane capacitance that reflect exocytosis. Effects of caffeine and ryanodine were found on the electrical activity recorded from the postsynaptic side (the vestibular nerve) indicating that CICR can indeed modulate the transfer of information at this synapse.
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V.K. Unni, S.S. Zakharenko, L. Zablow, A.J. DeCostanzo, and S.A. Siegelbaum Calcium release from presynaptic ryanodine-sensitive stores is required for long-term depression at hippocampal CA3-CA3 pyramidal neuron synapses J Neurosci 24 2004 9612 9622 Combining electrophysiology, presynaptic calcium measurements and styryl dye fluorescent measurements (as probes of exocytosis), the authors present strong evidence for a role of presynaptic calcium stores in long-term changes in synaptic efficacy. The use of whole-cell recording to load calcium stores blockers in the pre- versus the post- synaptic cell is a very important control, because in a brain slice multiple sites of action of these drugs are difficult to distinguish. Presynaptic calcium rises during the LTD induction protocol are shown to include a ryanodine-sensitive component, which has a slow rise phase. This component is required for LTD. Because this form of LTD involves retrograde signaling (postsynaptic NMDA receptors are required), the authors suggest that presynaptic RyRs serve to inform the terminal of its activity level, thus counteracting the diffuse action of the retrograde messenger.
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I.C. Duguid, and T.G. Smart Retrograde activation of presynaptic NMDA receptors enhances GABA release at cerebellar interneuron-Purkinje cell synapses Nat Neurosci 7 2004 525 533 A new form of retrograde signaling for the cerebellum is described in this interesting paper: calcium rises in the postsynaptic Purkinje cell lead to the release of glutamate that activates presynaptic NMDA-Rs. The result is a potentiation of GABAergic synaptic transmission. The effect is presynaptic: for mIPSCs, frequency increases; for evoked IPSCs, PPR decreases and the coefficient of variation increases. The involvement of presynaptic stores is suggested because ryanodine blocks the increase in mIPSC frequency.
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