Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast Pichia pastoris

Protein Expression and Purification

Volumn 42, Issue 1, 2005, Pages 20-28

  Ge, Tao ^a   Sun, Zhao Jun ^a   Fu, Shi Hong ^a   Liang, Guo Dong ^a  

^a Inst for Virus Dis Ctrl/Prev   (China)

Author keywords

Expression; Fibrinolytic; Lumbrokinase; Yeast

Indexed keywords

FIBRIN; LUMBROKINASE; PROTEINASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ANNELID WORM; ARTICLE; BIOSYNTHESIS; CHEMICAL STRUCTURE; ENZYMOLOGY; FIBRINOLYSIS; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PLASMID; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; ENDOPEPTIDASES; FIBRIN; FIBRINOLYSIS; GENE EXPRESSION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OLIGOCHAETA; PICHIA; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

LUMBRICUS BIMASTUS; PHERETIMA SIEBOLDI; PICHIA PASTORIS;

EID: 20444379332     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.04.005     Document Type: Article

References (25)

1. Y. Tang, D.C. Liang, T. Jiang, J.P. Zhang, L.L. Gui, and W.R. Chang Crystal structure of earthworm fibrinolytic enzyme component A: revealing the structural determinants of its dual fibrinolytic activity J. Mol. Biol. 321 2002 57 68
2. J.Y. Zhao, and T.Y. Jing The analysis of fibrinolytic enzyme from earthworm Chin. J. Biochim. Mol. Biol. 14 4 1998 407 411
3. N. Nakajima, M. Sugimoto, and K. Ishihara Stable earthworm serine proteases: application of the protease function and usefulness of the earthworm autolysate J. Biosci. Bioeng. 90 2000 174 179
4. H. Mihara, H. Sumi, T. Yoneta, H. Mizumoto, R. Ikeda, M. Seiki, and M. Maruyama A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus Jpn. J. Physiol. 41 4 1991 461 473
5. N. Nakajima, H. Mihara, and H. Sumi Characterization of potent fibrinolytic enzymes in earthworm: Lumbricus rubellus Biotech. Biochem. 57 1993 1726 1730
6. Q. Fan, C. Wu, L. Li, R. Fan, C. Wu, Q. Hon, and R. He Some features of intestinal absorption of intact fibrinolytic enzyme III-I from Lumbricus rubellus Biochim. Biophys. Acta. 1526 2001 286 292
7. F. Wang, C. Wang, M. Li, L. Gui, J. Zhang, and W. Chang Purification, characterization and crystallization of a group of earthworm fibrinolytic enzymes from Eisenia fetida Biotechnol. Lett. 25 13 2003 1105 1109
8. B.D. Xing, S.M. Yin, and B.G. Ru Purification and characterization of the fibrinolytic enzyme (eFE-D) from earthworm Eisenia folelide Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 29 6 1997 609 612
9. N. Nakajima, M. Sugimoto, K. Ishihara, K. Nakamura, and H. Hamada Further characterization of earthworm serine proteases: cleavage specificity against peptide substrates and on autolysis Biosci. Biotechnol. Biochem. 63 11 1999 2031 2033
10. L. Li, J. Zhao, and R.Q. He Isolation and some characterizations of a glycosylated fibrinolytic enzyme of earthworm, Eisenia fetida Protein Pept. Lett. 10 2 2003 183 190
11. X.H. Liu, and F. Ge Factors influencing the activity of fibrinolytic enzymes from earthworm, Eeisenia foetida Zhongguo Zhong Yao Za Zhi 27 6 2002 423 426
12. M. Popovic, T.M. Hrcenjak, T. Babic, J. Kos, and M. Grdisa Effect of earthworm (G-90) extract on formation and lysis of clots originated from venous blood of dogs with cardiopathies and with malignant tumors Pathol. Oncol. Res. 7 3 2001 197 202
13. C.M. Hwang, D.I. Kim, S.H. Huh, B.G. Min, J.H. Park, J.S. Han, B.B. Lee, Y.I. Kim, E.S. Ryu, and J.W. Kim In vivo evaluation of lumbrokinase, a fibrinolytic enzyme extracted from Lumbricus rubellus, in a prosthetic vascular graft J. Cardiovasc. Surg. (Torino). 43 6 2002 891 894
14. Y.S. Kim, M.K. Pyo, K.M. Park, B.S. Hahn, K.Y. Yang, and H.S. Yun-Choi Dose dependency of earthworm powder on antithrombiotic and fibrinolytic effects Arch. Pharm. Res. 21 4 1998 374 377
15. N. Nakajima, K. Ishihara, M. Sugimoto, H. Sumi, K. Mikuni, and H. Hamada Chemical modification of earthworm fibrinolytic enzyme with human serum albumin fragment and characterization of the protease as a therapeutic enzyme Biosci. Biotechnol. Biochem. 60 2 1996 293 300
16. T.Y. Zhang, J.X. Luo, and X.Y. Lu Cloning and expression of kringle 1-3 gene of human plasminogen and the purification and bioactivity of its expressed product Sheng Wu Gong Cheng Xue Bao 18 5 2002 593 596
17. P. Wang, J. Zhang, Z. Sun, Y. Chen, V. Gurewich, and J.N. Liu Catalytic and fibrinolytic properties of recombinant urokinase plasminogen activator from E. coli, mammalian, and yeast cells Thromb. Res. 100 5 2000 461 467
18. J. Pratap, G. Rajamohan, and K.L. Dikshit Characteristics of glycosylated streptokinase secreted from Pichia pastoris: enhanced resistance of SK to proteolysis by glycosylation Appl. Microbiol. Biotechnol. 53 4 2000 469 475
19. R.G. Miele, F.J. Castellino, and R.K. Bretthauer Characterization of the acidic oligosaccharides assembled on the Pichia pastoris-expressed recombinant kringle 2 domain of human tissue-type plasminogen activator Biotechnol. Appl. Biochem. 26 Pt 2 1997 79 83
20. S. Manabu, and N. Nobuyoshi Molecular cloning, sequencing, and expression of cDNA encoding serine protease with fibrinolytic activity from earthworm Biosci. Biotechnol. Biochem. 65 7 2001 1575 1580
21. D.R. Higgins, J.M. Cregg, Method in Molecular Biology, Vol 103, Pichia protocols, Humana Press Inc, Totowa, NJ
22. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 2nd 1989 Cold Spring Harbor Laboratory Press New York
23. Invitogen Corporation: a manual of methods for expression of recombinant proteins using pPICZ and pPICZα-A in Pichia pastoris. Catalog No.K1740-01, Version G, USA
24. M.M. Bradford A rapid and sensitive method for quantitation of microgram quantities of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
25. R. Hu, S. Zhang, H. Liang, N. Li, and C. Tu Codon optimization, expression, and characterization of recombinant lumbrokinase in goat milk Protein Expr. Purif. 37 1 2004 83 88