Alanine-scanning mutagenesis of HM-1 killer toxin and the essential residues for killing activity

Journal of Biochemistry

Volumn 137, Issue 4, 2005, Pages 517-522

  Miyamoto, Masahiko ^a   Han, Gi Dong ^a   Kimura, Tetsuya ^b   Furuichi, Yasuhiro ^c   Komiyama, Tadazumi ^a  

^a NIIGATA UNIVERSITY OF PHARMACY AND APPLIED LIFE SCIENCES   (Japan)

^b MIE UNIVERSITY   (Japan)

^c GeneCare Research Institute   (Japan)

Author keywords

Alanine scanning; HM 1; Killer toxin; Site directed mutagenesis

Indexed keywords

ALANINE; AMINO ACID; ASPARTIC ACID; HISTIDINE; HM 1 TOXIN; LYSINE; TOXIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; TOXICITY;

ALANINE; BASE SEQUENCE; INHIBITORY CONCENTRATION 50; MUTAGENESIS, SITE-DIRECTED; MYCOTOXINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

SACCHAROMYCES CEREVISIAE;

EID: 20444378918     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvi063     Document Type: Article

References (34)

1. Young, T.W. and Yagiu, M. (1978) A comparison of the killer character in different yeasts and its classification. Antonie van Leeuwenhoek 44, 59-77
2. Kimura, T., Kitamoto, N., Ohta, Y., Kito, Y., and Iimura, Y.J. (1995) Structural relationships among killer toxins secreted from the killer strains of the genus Williopsis. J. Ferment. Bioeng. 80, 85-87
3. Yamamoto, T., Imai, M., Tachibana, K., and Mayumi, M. (1986) Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted by Hansenula mrakii. FEBS Lett. 195, 253-257
4. Komiyama, T., Ohta, T., Urakami, H., Shiratori, Y., Takasuka, T., Satoh, M., Watanabe, T., and Furuichi, Y. (1996) Pore formation on proliferating yeast Saccharomyces cerevisiae cell buds by HM-1 killer toxin. J. Biochem. 119, 731-736
5. Debeno, M. and Gordee, R. (1994) Antibiotics that inhibit fungal cell wall development. Annu. Rev. Microbiol. 48, 471-497
6. Komiyama, T., Shirai, T., Ohta, T., Urakami, H., Furuichi, Y., Ohta, Y., and Tsukada, Y. (1998) Action properties of HYI killer toxin from Williopsis saturnus var. saturnus, and antibiotics, aculeacin A and papulacandine B. Biol. Pharm. Bull. 21, 1013-1019
7. Komiyama, T., Kimura, T., and Furuichi, Y (2002) Round shape enlargement of the yeast spheroplast of Saccharomyces cerevisiae by HM-1 toxin. Biol. Pharm. Bull. 25, 959-965
8. Kasahara, S., Inoue, S.B., Mio, T., Yamada, T., Nakajima, T., Ichishima, E., Furuichi, Y., and Yamada, H. (1994) Involvement of cell wall beta-glucan in the action of HM-1 killer toxin. FEBS Lett. 348, 27-32
9. Kimura, T., Iimura, Y., Komiyama, T., Karita, S., Sakka, K., and Ohmiya, K. (1998) Killing mechanism by HM-1 killer toxin of Hansenula mrakii and application of HM-1 in fermentation process to prevent contamination with wild yeasts. Recent Res. Dev. Ferment. Bioeng. 1, 225-240
10. Takasuka, T., Komiyama, T., Furuichi, Y., and Watanabe, T. (1995) Cell wall synthesis specific cytocidal effect of Hansenula mrakii toxin-1 on Saccharomyces cerevisiae. Cell. Mol. Biol. Res. 41, 575-581
11. Kimura, T., Kitamoto, Kito, N.Y., Iimura, Y., Shirai, T., Komiyama, T., Furuichi, Y., Sakka, K., and Ohmiya, K. (1997) A novel yeast gene, RHK1, is involved in the synthesis of the cell wall receptor for the HM-1 killer toxin that inhibits beta-1, 3-glucan synthesis. Mol. Gen. Genet. 254, 139-147
12. Kimura, T., Komiyama, T., Furuichi, Y., Iimura, Y., Karita, S., Sakka, K., and Ohmiya, K. (1999) N-glycosylation is involved in the sensitivity of Saccharomyces cerevisiae to HM-1 killer toxin secreted from Hansenula mrakii IFO 0895. Appl. Microbiol. Biotechnol. 51, 176-184
13. Kimura, T., Kitamoto, N. Matsuoka, K., Nakamura, K., Iimura, Y., and Kito, Y. (1993) Isolation and nucleotide sequence of the genes encoding killer toxins from Hansenula mrakii and H. saturnus. Gene 137, 265-270
14. Shirai, T., Kimura, T., Furuichi, Y., and Komiyama, T. (2000) The involvement of two specific arginine residues in the action of HM-1 killer toxin was deduced from site-directed mutagenesis. Biol. Pharm. Bull. 23, 998-1000
15. Ito, W., Ishiguro, H., and Kurosawa, Y. (1991) A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene 102, 67-70
16. Sambrook, J., Fritsh, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
17. Antuch, W., Güntert, P., and Wüthrich, K. (1996) Ancestral beta gamma-crystallin precursor structure in a yeast killer toxin. Nat. Struct. Biol. 3, 662-665
18. Komiyama, T., Zhang, Q., Miyamoto, M., Selvakumar, D., and Furuichi, Y. (2004) Monoclonal antibodies and sandwich ELISA for quantitation of HM-1 killer toxin. Biol. Pharm. Bull. 27, 691-693
19. Tsang, V.C., Peralta, J.M., and Simons, A.R. (1983) Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 92, 377-391
20. Schäger, H. and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379
21. Kishida, M., Tokunaga, M., Katayose, Y., Yajima, H., Kawamura-Watabe, A., and Hishinuma, F. (1996) Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 60, 798-801
22. Kowalski, J.M., Parekh, R.N., Mao, J., and Wittrup, K.D. (1998) Protein folding stability can determine the efficiency of escape from endoplasmic reticulum quality control. J. Biol. Chem. 273, 19453-19458
23. Ellgaard, L. and Helenius, A. (2003) Quality control in the endoplasmic reticulum. Nat. Rev. Mol. Cell. Biol. 4, 181-191
24. Anderson, D.E., Becktel, W.J., and Dahlquist, F.W. (1990) pH-induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme. Biochemistry 29, 2403-2408
25. Coll, M., Guasch, A., Aviles, F.X., and Huber, R. (1991) Three-dimensional structure of porcine procarboxypeptidase B: a structural basis of its inactivity. EMBO J. 10, 1-9
26. Langsetmo, K., Fuchs, J.A., Woodward, C., and Sharp, K.A. (1991) Linkage of thioredoxin stability to titration of ionizable groups with perturbed pKa. Biochemistry 30, 7609-7614
27. Cambillau, C., and Claverie, J.-M. (2000) Structural and genomic correlates of hyperthermostability. J. Biol. Chem. 275, 32383-32386
28. Lebbink, J.H.G., Consalvi, V., Chiaraluce, R., Berndt, K.D., and Ladenstein, R. (2002) Structural and thermodynamic studies on a salt-bridge triad in the NADP-binding domain of glutamate dehydrogenase from Thermotoga maritime: cooperativity and electrostatic contribution to stability. Biochemistry 41, 15524-15535
29. Buchut, G., Haggstrom, B., Radic, Z., Osterman, A., and Hjalmarsson, K. (1994) Residues important for folding and dimerisation of recombinant Torpedo californica acetylcholinesterase. Biochim. Biophys. Acta 1209, 265-273
30. Ohki, S., Kariya, E., Hiraga, K., Wakamiya, A., Isobe, T., Oda, K., and Kainosho, M. (2001) NMR structure of Streptomyces killer toxin-like protein, SKLP: further evidence for the wide distribution of single-domain betagamma-crystallin super family proteins. J. Mol. Biol. 305, 109-120
31. Bussey, H. (1981) Physiology of killer factor in yeast. Adv. Microb. Physiol. 22, 93-122
32. Martinac, B., Zhu, H., Kubalski, A., and Zhou, X. (1990) Yeast K1 killer toxin forms ion channels in sensitive yeast spheroplasts and in artificial liposomes. Proc. Natl Acad. Sci. USA 87, 6228-6232
33. Kurzweilova, H. and Sigler, K. (1994) Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations. Arch. Microbiol. 162, 211-214
34. Komiyama, T., Ohta, T., Furuichi, Y., Ohta, Y., and Tsukada, Y. (1995) Structure and activity of HYI killer toxin from Hansenula saturnus. Biol. Pharm. Bull. 18, 1057-1059