Direct tuber testing for Potato Y potyvirus by real-time RT-PCR and ELISA: Reliable options for post-harvest testing?

EPPO Bulletin

Volumn 35, Issue 1, 2005, Pages 93-97

  Fox, A ^a   Evans, F ^a   Browning, I ^a  

^a Science and Advice for Scottish Agriculture   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

APHIDIDAE; PIPERACEAE; POTATO VIRUS Y; POTYVIRUS; SOLANUM TUBEROSUM;

EID: 20444378210     PISSN: 02508052     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2338.2005.00805.x     Document Type: Conference Paper

References (17)

1. Barker H, Webster KD & Reavy B (1993) Detection of potato virus Y in potato tubers: a comparison of polymerase chain reaction and enzyme-linked immunosorbent assay. Potato Research 36, 13-20.
2. Bokx de JA (1981) Detection of potato leaf roll virus and potato viruses A and Y in early-lifted potato tubers. Potato Research 24, 236.
3. Bokx de JA & Cuperus C (1987) Detection of potato virus Y in early-harvested potato tubers by cDNA hybridization and three modifications of ELISA. Bulletin OEPP/EPPO Bulletin 17, 73-79.
4. Boonham N, Walsh K, Mumford R & Barker I (2000) Use of multiplex real-time PCR (TaqMan) for the detection of potato viruses. Bulletin OEPP/EPPO Bulletin 30, 427-430.
5. Boonham N, Walsh K, Swan H, Mumford R & Barker I (2001) Diagnosis or detection - developing appropriate methods. Proceedings of EAPR Virology Section Meeting 2001. Trest (CZ).
6. Browning IA, Fox A & Evans F (2004) Direct tuber testing for potato virus Y (PVY) by ELISA and real-time PCR. Proceedings of Crop Protection in Northern Britain 2004, 321-325.
7. Denny FE (1945) Synergistic effects of three chemicals in the treatment of dormant potatoes to hasten germination. Contributions Boyce Thompson Institute for Plant Research 14, 1-14.
8. Gugerli P (1979) Le test immuno-enzymatique (ELISA) et son application pour le diagnostic rapide de viroses de la pomme de terre. Revue Suisse Agricole 11, 253-260.
9. Gugerli P & Gehriger W (1980) Enzyme-linked immunosorbent assay (ELISA) for the detection of potato leafroll virus and potato virus Y in potato tubers after artificial break of dormancy. Potato Research 23, 353-359.
10. Hill SA & Jackson EA (1984) An investigation of the reliability of ELISA as a practical test for detecting potato leaf roll virus and potato virus Y in tubers. Plant Pathology 33, 21-26.
11. Jones DAC & Barker H (1998) Development of improved diagnostic ELISA tests for potato viruses Y, S and A in a post-harvest tuber testing scheme. Unpublished technical report from Scottish Executive Flexible Fund Projects FF413 and FF504.
12. Klerks MM, Leone GOM, Verbeek M, van den Heuvel JFJM & Schoen CD (2001) Development of a multiplex AmpliDet RNA for the simultaneous detection of potato leaf roll virus and potato virus Y in potato tubers. Journal of Virological Methods 93, 115-125.
13. Kurppa A & Tiilikkala K (1987) Potato virus Y infection in potato crops and tuber yields in relation to seed infection levels and early detection of the virus by ELISA. Abstracts of EAPR meeting 1987. Aalborg (DK).
14. Mumford RA, Walsh K, Barker I & Boonham N (2000) Detection of potato mop-top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90, 448-453.
15. Reust W & Gugerli P (1984) Oxygen and carbon dioxide treatment to break potato tuber dormancy for the reliable detection of potato virus Y (PVY) by enzyme-linked immunosorbent assay (ELISA). Potato Research 27, 435-439.
16. Singh M & Singh RP (1996) Factors affecting detection of PVY in dormant tubers by reverse transcription polymerase chain reaction and nucleic acid spot hybridization. Journal of Virological Methods 60, 47-57.
17. Vetten HJ, Ehlers U & Paul HL (1983) Detection of potato virus Y and A in tubers by enzyme-linked immunosorbent assay after natural and artificial break of dormancy. Phytopathologische Zeitschrift 107, 37-46.