Bacterial extraction from grapevine and detection of Xylophilus ampelinus by a PCR and Microwell plate detection system

EPPO Bulletin

Volumn 35, Issue 1, 2005, Pages 55-60

  Manceau, C ^a   Grall, S ^a   Brin, C ^a   Guillaumes, J ^a  

^a UNIVERSITÉ D'ANGERS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); VITIS; XYLOPHILUS AMPELINUS;

EID: 20444368858     PISSN: 02508052     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2338.2005.00813.x     Document Type: Article

References (10)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman SG, Smith JA & Struhl K. (1987) Preparation and analysis of DNA. In: Current Protocols in Molecular Biology (Suppl. 25): 2.1.5-2.1.6. Wiley, New York (US).
2. Botha WJ, Serfontein S, Greyling MM & Berger DK (2001) Detection of Xylophilus ampelinus in grapevine cuttings using a nested polymerase chain reaction. Plant Pathology 50, 515-526.
3. Edwards K, Johnstone C & Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for the PCR analysis. Nucleic Acids Research 19, 1349.
4. EU (2000) Council Directive 2000/29 of 8 July 2000 on protective measures against the introduction into the Member States of organisms harmful to plants or plant products. Official Journal of the European Communities L169, 1-112.
5. Gorris MT, Cambra M & López MM (1989) Production of monoclonal antibodies specific to Xylophilus ampelinus. In: Proceedings of the 7th International Conference on Plant Pathogenic Bacteria (Ed. Klement Z), pp. 913-921. Budapest (HU).
6. Manceau C, Coutaud MG & Guyon R (2000) Assessment of subtractive hybridization to select species and subspecies-specific DNA fragments for the identification of Xylophilus ampelinus by polymerase chain reaction (PCR). European Journal of Plant Pathology 106, 243-253.
7. Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor (US).
8. Ridé M, Ridé S, Rat B & Novoa D (1977) La nécrose bactérienne de la vigne: expérimentations préliminaires à une meilleure compréhension de la maladie. Bulletin Technique Pyrénées-Orientales 82, 1-25.
9. Serfontein S, Serfontein JJ, Botha WJ & Staphorst JL (1997) The isolation and characerization of Xylophilus ampelinus. Vitis 36, 209-210.
10. Willems A, Gillis M, Kersters K, van den Broeke L & de Ley J (1987) Transfer of Xanthomonas ampelina Panagopoulos 1969 to a new genus, Xylophilus gen. nov., as Xylophilus ampelinus (Panagopoulos 1969) comb. nov. International Journal of Systematic Bacteriology 37, 422-430.