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Volumn 32, Issue 1, 2000, Pages 29-30

Construction and characterization of a mutant of single-chain urokinase-type Plasminogen Activator (Ser175-His187-mscu-PA)

Author keywords

Intrinsic catalytic activity; Single chain urokinase type plasminogen activator; Site specific mutagenesis; Zymogen triad

Indexed keywords


EID: 20444368848     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (3)

References (8)
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  • 2
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    • Fibrinolytic properties of one-chain and two-chain human extrinsic (tissue-type) plasminogen activator
    • Rijken D C, Hoylaerts M, Collen D. Fibrinolytic properties of one-chain and two-chain human extrinsic (tissue-type) plasminogen activator. J Biol Chem, 1982, 257: 2920-2925
    • (1982) J Biol Chem , vol.257 , pp. 2920-2925
    • Rijken, D.C.1    Hoylaerts, M.2    Collen, D.3
  • 3
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    • Inactivation of single-chain urokinase (prourokinase) by thrombin and thrombin-like enzymes, relevance of the findings to the interpretation of fibrin-binding experiments
    • Pannell R, Gurewich V. Inactivation of single-chain urokinase (prourokinase) by thrombin and thrombin-like enzymes, relevance of the findings to the interpretation of fibrin-binding experiments. Blood, 1987, 69: 22-26
    • (1987) Blood , vol.69 , pp. 22-26
    • Pannell, R.1    Gurewich, V.2
  • 4
    • 0029825436 scopus 로고    scopus 로고
    • A site-directed mutagenesis of pro-urokinase which substantially reduces its intrinsic activity
    • Liu J N, Tang W, Sun Z Y, Kung W, Pannell R, Sarmientos P, Gurewich V. A site-directed mutagenesis of pro-urokinase which substantially reduces its intrinsic activity. Biochemistry, 1996, 35: 14070-14076
    • (1996) Biochemistry , vol.35 , pp. 14070-14076
    • Liu, J.N.1    Tang, W.2    Sun, Z.Y.3    Kung, W.4    Pannell, R.5    Sarmientos, P.6    Gurewich, V.7
  • 5
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    • Purification and characterization of recombinant single-chain urokinase produced in Escherichia coli
    • Winkler M E, Blaber M. Purification and characterization of recombinant single-chain urokinase produced in Escherichia coli. Biochemistry, 1986, 25: 4041-4045
    • (1986) Biochemistry , vol.25 , pp. 4041-4045
    • Winkler, M.E.1    Blaber, M.2
  • 6
    • 0027475969 scopus 로고
    • The kinetics of plasminogen activation by thrombin-cleaved pro-urokinase and promotion of its activity by fibrin fragment E-2 and by tissue plasminogen activator
    • Liu J, Gurewich V. The kinetics of plasminogen activation by thrombin-cleaved pro-urokinase and promotion of its activity by fibrin fragment E-2 and by tissue plasminogen activator. Blood, 1993, 81: 980-987
    • (1993) Blood , vol.81 , pp. 980-987
    • Liu, J.1    Gurewich, V.2
  • 7
    • 49749198019 scopus 로고
    • Urokinase: An activator of plasminogen from human urine, isolation and properties
    • Ploug T, Kjeldgaard N O. Urokinase: An activator of plasminogen from human urine, isolation and properties. Biochem Biophys Acta, 1957, 24: 278-282
    • (1957) Biochem Biophys Acta , vol.24 , pp. 278-282
    • Ploug, T.1    Kjeldgaard, N.O.2
  • 8
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    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680-685
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    • Laemmli, U.K.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.