Uniform particles for the reversed-phase separation of proteins with high-resolution and high-column efficiency

Separation Science and Technology

Volumn 40, Issue 7, 2005, Pages 1425-1438

  Unsal, Ender ^a   Elmas, Begum ^a   Camli, S Tolga ^a   Senel, Serap ^a   Tuncel, Ali ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Chromatographic packing; Divinylbenzene; HPLC; Porous particles; Protein separation; Reversed phase chromatography; Uniform particles

Indexed keywords

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; PACKING; PHASE SEPARATION; POLYMERIZATION; TOLUENE;

HIGH-RESOLUTION LIQUID CHROMATOGRAMS; REVERSED-PHASE SEPARATION; SYNTHESIZED BEADS; THEORETICAL PLATE NUMBERS (TPN);

PROTEINS;

SEPARATION;

EID: 20344389536     PISSN: 01496395     EISSN: None     Source Type: Journal    
DOI: 10.1081/SS-200053011     Document Type: Article

References (34)

1. Lowe, C.R., Glad, M., Larsson, P.O., Ohlson, S., Small, D.A.P., Atkinson, T., and Mossbach, K. (1981) High-performance liquid affinity chromatography of proteins on cibacron blue F3G-A bonded silica. Journal of Chromatography, 215 (2): 303-316.
2. Lee, W.C. (1997) Protein separation using non-porous sorbents. Journal of Chromatography B, Biomedical Applications, 699 (1): 29-45.
3. Leonard, M., Fournier, C., and Dellacherie, E. (1995) Polyvinyl alcohol-coated macroporous polystyrene particles as stationary phases for the chromatography of proteins. Journal of Chromatography B, Biomedical Applications, 664 (1): 39-46.
4. Nash, D.C., McCreath, G.E., and Chase, H.A. (1997) Modification of polystyrenic matrices for the purification of proteins effect of the adsorption of poly(vinyl alcohol) on the characteristics of poly(styrene-divinylbenzene) beads for use in affinity chromatography. Journal of Chromatography A, 758 (1): 53-64.
5. Camli, S.T., Senel, S., and Tuncel, A. (1999) Cibacron blue F3G-A-attached uniform and macroporous poly(styrene-co-divinylbenzene) particles for specific albumin adsorption. Journal of Biomaterials Science Polymer Edition, 10 (8): 875-889.
6. Ellingsen, T., Aune, O., Ugelstad, J., and Hagen, S. (1990) Monosized stationary phases for chromatography. Journal of Chromatography, 535 (1): 147-161.
7. Cheng, C.M., Micale, F.J., Vanderhoff, J.W., and El-Aasser, M.S. (1992) Synthesis and characterization of monodisperse porous polymer particles. Journal of Polymer Science: Part A: Polymer Chemistry, 30 (2): 235-244.
8. Cheng, C.M., Vanderhoff, J.W., and El-Aasser, M.S. (1992) Monodisperse porous polymer particles: Formation of the porous structure. Journal of Polymer Science: Part A: Polymer Chemistry, 30 (2): 245-256.
9. Galia, M., Svec, F., and Frechet, J.M.J. (1994) Monodisperse pollymer beads as packing material for high-performance liquid chromatography: Effect of divinylbenzene content on the porous and chromatographic properties of poly(styrene-co-divinylbenzene) beads prepared in presence of linear polystyrene as a porogen. Journal of Polymer Science: Part A: Polymer Chemistry, 32 (14): 2169-2175.
10. Wang, Q.C., Svec, F., and Frechet, J.M.J. (1994) Fine control of the porous structure and chromatographic properties of monodisperse macroporous poly (styrene-co-divinyl benzene) beads using polymer porogens. Journal of Polymer Science: Part A: Polymer Chemistry, 32 (15): 2577-2588.
11. Tuncel, A., Tuncel, M., and Salih, B. (1999) Electron microscopic observation of uniform porous particles. I. Effect of seed latex type and diluent. Journal of Applied Polymer Science, 71 (14): 2271-2290.
12. Tuncel, A. (1999) Electron microscopic observation of uniform macroporous particles. II. Effect of DVB concentration. Journal of Applied Polymer Science, 71 (14): 2291-1302.
13. Tuncel, A., Unsal, E., Camli, ST., and Senel, S. (2003) Polymer beads in biomedical chromatography. In Colloidal Biomolecules, Biomaterials, and Biomedical Applications; Elaissari, A., ed.; Marcel Dekker: New York, 161-187.
14. Li, W.H. and Stöver, H.D.H. (2000) Monodisperse cross-linked core-shell polymer microspheres by precipitation polymerization. Macromolecules, 33 (12): 4354-4360.
15. Ogino, K., Sato, H., Tsuchiya, K., Suzuki, H., and Moriguchi, S. (1995) Synthesis of monodisperse macroreticular styrene-divinylbenzene gel particles by a single-step swelling and polymerization method. Journal of Chromatography A, 699 (1-2): 59-66.
16. Ogino, K., Sato, H., Aihara, Y., Suzuki, H., and Moriguchi, S. (1995) Preparation and characterization of monodisperse oligo(ethylene glycol) dimethacrylate polymer beads for aqueous high-performance liquid chromatography. Journal of Chromatography A, 699 (1-2): 67-72.
17. Ericson, C. and Hjerten, S. (1999) Reversed-phase electrochromatography of proteins on modified continuous beds using normal-flow and counterflow gradients. Theoretical and Practical Considerations. Analytical Chemistry, 71 (8): 1621-1627.
18. Li, Y.M., Liao, J.L., Zhang, R., Henriksson, H., and Hjerten, S. (1999) Continuous beds for microchromatography: Chromatofocusing and anion exchange chromatography. Analytical Biochemistry, 267 (1): 121-124.
19. Podgornik, A., Barut, M., Jaksa, S., Sancar, J., and Strancar, A. (2002) Application of very short monolithic columns for separation of low and high molecular mass substances. Journal of Liquid Chromatography & Related Technologies, 25 (20): 3099-3116.
20. Josic, D. and Buchacher, A. (2001) Application of monoliths as supports for affinity chromatography and fast enzymatic conversion. Journal of Biochemical and Biophysical Methods, 49 (1-3): 153-174.
21. Zhang, S., Zhang, J., and Horvath, C. (2002) Capillary electrochromatography of proteins with polymer-based strong-cation-exchanger microspheres. Journal of Chromatography A, 965 (1-2): 83-92.
22. Paredes, B., Suarez, E., Rendueles, M., Villa-Garcia, M.A., and Diaz, J.M. (2001) Ammonia-functionalized particulate poly(glycidyl methacrylate-co-ethylene dimethacrylate)-based polymers as stationary phase for protein retention. Journal of Chemical Technology and Biotechnology, 76 (11): 1171-1178.
23. Shi, Y. and Sun, Y. (2003) Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography. Chromatographia, 57 (1-2): 29-35.
24. Vankova, H., Kucerova, Z., and Turkova, J. (2001) Reversed-phase high-performance liquid chromatography of peptides of porcine pepsin prepared by the use of various forms of immobilized alpha-chymotrypsin. Journal of Chromatography B, 735 (1): 37-43.
25. Gong, B., Ke, C., and Geng, X. (2003) Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatographic properties. Analytical and Bioanalytical Chemistry, 375 (6): 769-774.
26. Gong, B., Wang, L., Wang, C., and Geng, X. (2004) Preparation of hydrophobic interaction chromatographic packings based on monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads and their application. Journal of Chromatography A, 1022 (1-2): 33-39.
27. Camli, T., Tuncel, M., Senel, S., and Tuncel, A. (2002) Functional, uniform and macroporous latex particles: Preparation, electron microscopic characterization, and nonspecific protein adsorption properties. Journal of Applied Polymer Science, 84(2): 414-429.
28. Tuncel, A., Tuncel, M., Ergun, B., Alagöz, C., and Bahar, T. (2002) Carboxyl carrying-large uniform latex particles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 197 (1-3): 79-94.
29. Tuncel, A., Tuncel, M., Cicek, H., and Fidanboy, O. (2001) 2-Hydroxyethylmethacrylate carrying uniform porous particles: Preparation and electron microscopy. Polymer International, 51 (1): 75-84.
30. Senel, S., Çamli, S.T., and Tuncel, A. (2002) Nucleotide adsorption-desorption behaviour of boronic acid functionalized uniform-porous particles. Journal of Chromatography B, 769 (2): 283-295.
31. Unsal, E., Camli, S.T., Senel, S., and Tuncel, A. (2004) Chromatographic performance of monodisperse-macroporous particles produced by "modified seeded polymerization" I: Effect of monomer/seed latex ratio. Journal of Applied Polymer Science, 92 (1): 607-618.
32. Camli, S.T., Unsal, E., Senel, S., and Tuncel, A. (2004) Chromatographic performance of monodisperse macroporous particles produced by modified seeded polymerization. II. Effect of diluent/seed-latex ratio. Journal of Applied Polymer Science, 92 (6): 3685-3696.
33. Unsal, E., Camli, S.T., Elmas, B., Tuncel, M., Senel, S., and Tuncel, A. (2004) Monodisperse-porous poly(styrene-divinyl benzene) beads providing high column efficiency in reversed phase HPLC. Journal of Applied Polymer Science, In Press.
34. Ferreira, A., Bigan, M., and Blondeau, D. (2003) Optimization of a polymeric HPLC phase: Poly(glycidyl methacrylate-co-ethylene dimethacrylate) influence of the polymerization conditions on the pore structure of macroporous beads. Reactive and Functional Polymers, 56 (2): 123-136.