Phenotypic and genotypic characterization of sorbitol-negative or slow-fermenting (suspected O157) Escherichia coli isolated from milk samples in Lombardy region

Letters in Applied Microbiology

Volumn 40, Issue 6, 2005, Pages 491-496

  Picozzi, Claudia ^a   Foschino, R ^a   Heuvelink, A ^b   Beumer, R ^c  

^a UNIVERSITY OF MILAN   (Italy)

^b Inspectorate for Health Protection   (Netherlands)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Antibiotic resistance; Escherichia coli O157; PCR; PFGE; Shiga toxin

Indexed keywords

ALCOHOLS; ANTIBIOTICS; ELECTROPHORESIS; ESCHERICHIA COLI; FERMENTED MILK; POLYMERASE CHAIN REACTION; STRAIN; SULFUR COMPOUNDS;

ANTIBIOTICS RESISTANCE; CULTURAL METHODS; ESCHERICHIA COLI O157; GENOTYPIC CHARACTERIZATION; MILK SAMPLE; PCR; PHENOTYPIC CHARACTERIZATION; PULSED FIELD GEL ELECTROPHORESIS; SEROGROUPS; SHIGA TOXIN;

GENES;

SHIGA TOXIN; SORBITOL;

MICROBIOLOGY;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM ISOLATION; ESCHERICHIA COLI; ESCHERICHIA COLI O157; GENOTYPE; GOAT; HEMOLYTIC UREMIC SYNDROME; MILK; NONHUMAN; PATHOGENICITY; PHENOTYPE; POLYMERASE CHAIN REACTION; SAMPLING; STRAIN DIFFERENCE;

CAPRA HIRCUS; ESCHERICHIA COLI;

EID: 20044365257     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2005.01718.x     Document Type: Article

References (23)

1. Acheson, D.W.K., Lincicome, L.L., De Breucker, S. and Keusch, G.T. (1996) Detection of Shiga-like toxin-producing Escherichia coli in ground beef and milk by commercial enzyme immunoassay. J Food Prot 59, 344-349.
2. Ackers, M.L., Mahon, B.E., Leahy, E., Goode, B., Damrow, T., Hayes, P.S., Bibb, W.F., Rice, D.H. et al. (1988) An outbreak of Escherichia coli O157:H7 infections associated with leaf lettuce consumption. J Infect Dis 117, 1588-1593.
3. Antibiogram Committee of the French Society for Microbiology (1999) Antibiogram Committee of the French Society for Microbiology: official statement. Pathol Biol 47, 845-872.
4. Bastian, S.N., Carle, I. and Grimont, F. (1998) Comparison of 14 PCR systems for the detection and subtyping of stx genes in Shiga-toxin-producing Escherichia coli. Res Microbiol 149, 457-472.
5. Bielaszewska, M., Janda, J., Bláhová, K., Minařiková, H., Jíková, E., Karmali, M.A., Laubová, J., Šikulová, J. et al. (1997) Human Escherichia coli O157:H7 infection associated with the consumption of unpasteurized goat's milk. Epidemiol Infect 119, 299-305.
6. Bielaszewska, M., Schmidt, H., Karmali, M.A., Khakhria, R., Janda, J., Bláhová, K. and Karch, H. (1998) Isolation and characterization of sorbitol-fermenting Shiga-toxin (verocytotoxin)-producing Escherichia coli O157:H- strains in the Czech Republic. J Clin Microbiol 36, 2135-2137.
7. Boyce, T.G., Swerdlow, D.L. and Griffin, P.M. (1995) Escherichia coli O157:H7 and the hemolytic-uremic syndrome. N Engl J Med 333, 364-368.
8. Center for Disease Control and Prevention (CDC) (1998) PulseNet - The National Molecular Subtyping Network for Foodborne and Disease Surveillance. One Day Standard Protocol for Molecular Subtyping of Escherichia coli O157:H7 by PFGE. Atlanta, GA: Centers for Disease Control.
9. Cobbold, R. and Desmarchelier, P. (2001) Characterisation and clonal relationships of Shiga-toxigenic Escherichia coli (STEC) isolated from Australian dairy cattle. Vet Microbiol 79, 323-335.
10. 2), intimin (eaeA), and enterohemorragic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal faeces by multiplex PCR. Appl Environ Microbiol 65, 868-872.
11. Fratamico, P.M., Sackitey, S.K., Wiedmann, M. and Deng, M.Y. (1995) Detection of Escherichia coli O157:H7 by multiplex PCR. J Clin Microbiol 33, 2188-2191.
12. Gannon, V.P.J., King, R.K., Kim, J.Y. and Golsteyn Thomas, E.J. (1992) Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction. Appl Environ Microbiol 58, 3809-3815.
13. Gannon, V.P.J., D'Souza, S., Graham, T., King, R.K., Rahn, K. and Read, S. (1997) Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorragic Escherichia coli strains. J Clin Microbiol 35, 656-662.
14. Heuvelink, A.E., van de Kar, N.C., Meis, J.F., Monnens, L.A. and Melchers, W.J. (1995) Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uraemic syndrome in Western Europe. Epidemiol Infect 115, 1-14.
15. Heuvelink, A.E., Zwartkruis-Nahuis, J.T.M. and de Boer, E. (1997) Evaluation of Media and test kits for the detection and isolation of Escherichia coli O157 from minced beef. J Food Prot 60, 817-824.
16. Heuvelink, A.E., van de Biggelaar, F.L.A.M., De Boer, E., Herbes, G., Melchers, W.J.G., Huis in't Veld, J.H.J. and Monnens, L.A.H. (1998) Isolation and characterisation of verotoxic-producing Escherichia coli O157 strains from Dutch cattle and sheep. J Clin Microbiol 36, 878-882.
17. Law, D. (2000) Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli. J Appl Microbiol 88, 729-745.
18. March, S.B. and Ratnam, S. (1986) Sorbitol-MacConkey medium for the detection of Escherichia coli O157:H7 associated with haemorrhagic colitis. J Clin Microbiol 23, 869-872.
19. Nataro, J.P. and Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clin Microbiol Rev 11, 142-201.
20. National Committee for Clinical Laboratory Standards (NCCLS) (2003) Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, 8th edn. NCCLS document M2-A8. Wayne, PA: NCCLS.
21. Okrend, A.J.G., Rose, B.E. and Matner, E. (1990) An improved screening method for the detection and isolation of Escherichia coli O157:H7 from meat, incorporating the 3 M Petrifilm™ Test Kit-HEC for hemorrhagic Escherichia coli O157:H7. J Food Prot 53, 936-940.
22. Radu, S., Wai Ling, O., Karim, M.I.A. and Nishibuchi, M. (2001) Detection of Escherichia coli O157 by multiplex PCR and their characterization by plasmid profiling, antimicrobial resistance, RAPD and PFGE analyses. J Microbiol Methods 46, 131-139.
23. Tozzi, A.E., Caprioli, A., Minelli, F., Gianviti, A., De Petris, L., Edefonti, A., Montini, G., Ferretti, A. et al. (2003) Shiga toxin-producing Escherichia coli infections associated with hemolytic uremic syndrome, Italy, 1988-2003. Emerg Infect Dis 9, 106-108.