Molecular beacon aptamers for protein monitoring in real-time and in homogeneous solutions

Current Proteomics

Volumn 2, Issue 1, 2005, Pages 31-40

  Cao, Zehui ^a   Suljak, Steven W ^a   Tan, Weihong ^a  

^a University of Florida ^*  (United States)

Author keywords

Aptamers; Molecular beacon; Protein monitoring; Proteomics

Indexed keywords

EID: 19944419475     PISSN: 15701646     EISSN: None     Source Type: Journal    
DOI: 10.2174/1570164053507763     Document Type: Article

References (26)

1. Aebersold, R. and Mann, M. (2003). Mass spectrometry-based proteomics. Nature 422:198-207.
2. Bronzent, D.A., Pantazis, P., Antoniades, H.N., Kasid, A., Davidson, N., Dickson, R.B. and Lippman, M.E. (1987). Synthesis and secretion of platelet-derived growth-factor by human-breast cancer cell-lines. Proc. Natl. Acad. Sci. USA 84: 5763-7.
3. Cao, Z. (2002). Using fluorescence anisotropy for sensitive platelet-derived growth factor detection based on molecular aptamers. Master thesis. University of Florida.
4. Ellington, A.D. and Szostak, J.W. (1990). In vitro selection of RNA molecules that bind specific ligands. Nature 346: 818-22.
5. Fang, X., Cao, Z., Beck, T. and Tan, W. (2001). Molecular aptamer for real-time oncoprotein platelet-derived growth factor monitoring by fluorescence anisotropy. Anal. Chem. 73: 5752-7.
6. Fang, X., Sen, A., Vicens, M. and Tan, W. (2003). Synthetic DNA aptamers to detect protein molecular variants in a high-throughput fluorescence quenching assay. Chembiochem. 4: 829-34.
7. Fenn, J.B., Mann, M., Meng, C.K., Wong, S.F. and Whitehouse, C.M. (1989). Electrospray ionization for mass spectrometry of large biomolecules. Science 246: 64-71.
8. Fredriksson, S., Gullberg, M., Jarvius, J., Olsson, C., Pietras, K., Gustafsdottir, S.M., Ostman, A. and Landegren, U. (2002). Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 20: 473-7.
9. Gold, L. (1995). Oligonucleotides as research, diagnostic, and therapeutic agents. J. Biol. Chem. 270: 13581-4.
10. Green, L.S., Jellinek, D., Jenison, R., Ostman, A., Heldin, C.H. and Janjic, N. (1996). Inhibitory DNA ligands to platelet-derived growth factor B-chain. Biochemistry 35: 14413-24.
11. Hamaguchi, N., Ellington, A. and Stanton, M. (2001). Aptamer beacons for the direct detection of proteins. Anal. Biochem. 294: 126-31.
12. Jayasena, S.D. (1999). Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin. Chem. 45: 1628-50.
13. Karas, M. and Hillenkamp, F. (1988). Laser desorption ionization of proteins with molecular masses exceeding 10000 daltons. Anal. Chem. 60: 2299-301.
14. Li, J.W.J., Fang, X.H., Schuster, S.M. and Tan, W.H. (2000). Molecular beacons: A novel approach to detect protein - DNA interactions. Angew. Chem. Int. Edit. 39: 1049-52.
15. Li, J.W.J., Fang, X.H. and Tan, W.H. (2002). Molecular aptamer beacons for real-time protein recognition. Biochem. Biophys. Res. Comm. 292: 31-40.
16. Macaya, R.F., Schultze, P., Smith, F.W., Roe, J.A. and Feigon, J. (1993). Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution. P. Natl. Acad. Sci. USA 90: 3745-9.
17. MacBeath, G. and Schreiber, S.L. (2000). Printing proteins as microarrays for high-throughput function determination. Science 289: 1760-3.
18. Padmanabhan, K., Padmanabhan, K.P., Ferrara, J.D., Sadler, J.E. and Tulinsky, A. (1993). The structure of alpha-thrombin inhibited by a 15-mer single-stranded-DNA aptamer. J. Biol. Chem. 268: 17651-4.
19. Pandey, A. and Mann, M. (2000). Proteomics to study genes and genomes. Nature 405: 837-46.
20. Stojanovic, M.N., de Prada, P. and Landry, D.W. (2000). Fluorescent sensors based on aptamer self-assembly. J. Am. Chem. Soc. 122: 11547-8.
21. Tuerk, C. and Gold, L. (1990). Systematic evolution of ligands by exponential enrichment - RNA ligands to bacteriophage-t4 DNA-polymerase. Science 249: 505-10.
22. Wang, K.Y., McCurdy, S., Shea, R.G., Swaminathan, S. and Bolton, P.M. (1993). A DNA aptamer which binds to and inhibits thrombin exhibits a new structural motif for DNA. Biochemistry 32:1899-904.
23. Wu, Q., Tsiang, M. and Sadler, J.E. (1992). Localization of the single-stranded DNA binding site in the thrombin anion-binding exosite. J. Biol. Chem. 267: 24408-12.
24. Xu, W. and Ellington, A.D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proc. Natl. Acad. Sci. USA 93: 7475-80.
25. Yamamoto, R. and Kumar, P.K.R. (2000). Molecular beacon aptamer fluoresces in the presence of Tat protein of HIV-1. Genes Cells 5: 389-96.
26. Zhang, P., Beck, T., and Tan, W. (2001). Design of a molecular beacon DNA probe with two fluorophores. Angew. Chem. Int. Edit. 40: 402-5.