Lifetime and diffusion of singlet oxygen in a cell

Journal of Physical Chemistry B

Volumn 109, Issue 18, 2005, Pages 8570-8573

  Skovsen, Esben ^a   Snyder, John W ^a   Lambert, John D C ^a   Ogilby, Peter R ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DEATH; CELLULAR DOMAIN; ELECTRONIC STATE; INTRACELLULAR IMAGING;

CELL MEMBRANES; CELLS; DIFFUSION; ERROR ANALYSIS; OXYGEN; QUENCHING;

MOLECULAR STRUCTURE;

SINGLET OXYGEN;

ARTICLE; CHEMISTRY; DIFFUSION;

DIFFUSION; SINGLET OXYGEN;

EID: 19944404727     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp051163i     Document Type: Article

References (28)

1. Foote, C. S.; Clennan, E. L. In Active Oxygen in Chemistry; Foots, C. S., Valentine, J. S., Greenberg, A., Liebman, J. F., Eds.; Chapman and Hall: London, 1995; pp 105-140.
2. Kochevar, I. E.; Lynch, M. C.; Zhuang, S.; Lambert, C. R. Photochem. Photobiol. 2000, 72, 548-553.
3. Dougherty, T. J.; Gomer, C. J.; Henderson, B. W.; Jori, G.; Kessel, D.; Korbelik, M.; Moan, J.; Peng, Q. J. Natl. Cancer Inst, 1998, 90, 889-905.
4. Oleinick, N. L.; Morris, R. L.; Belichenko, I. Photochem. Photobiol. Sci. 2002, 1, 1-21.
5. Ogilby, P. R. Acc. Chem. Res. 1999, 32, 512-519.
6. Schweitzer, C.; Schmidt, R. Chem. Rev. 2003, 103, 1685-1757.
7. Gorman, A. A.; Rodgers, M. A. J. J. Photochem. Photobiol. B, Biol. 1992, 14, 159-176.
8. Baker, A.; Kanofsky, J. R. Photochem. Photobiol. 1993, 57, 720-727.
9. Niedre, M.; Patterson, M. S.; Wilson, B. C. Photochem. Photobiol. 2002, 75, 382-391.
10. Zebger, I.; Snyder, J. W.; Andersen, L. K.; Poulsen, L.; Gao, Z.; Lambert, J. D. C.; Kristiansen, U.; Ogilby, P. R. Photochem. Photobiol. 2004, 79, 319-322.
11. Frederiksen, P. K.; McIlroy, S. P.; Nielsen, C. B.; Nikolajsen, L.; Skovsen, E.; Jørgensen, M.; Mikkelsen, K. V.; Ogilby, P. R. J. Am. Chem. Soc. 2005, 727, 255-269.
12. Villanueva, A.; Caggiari, L.; Jori, G.; Milanesi, C. J. Photochem. Photobiol., B, Biol. 1994, 23, 49-56.
13. Georgiou, G. N.; Ahmet, M. T.; Houlton, A.; Silver, J.; Cherry, R. J. Photochem. Photobiol. 1994, 59, 419-422.
14. Snyder, J. W.; Zebger, L; Gao, Z.; Poulsen, L.; Frederiksen, P. K.; Skovsen, E.; Mcllroy, S. P.; Klinger, M.; Andersen, L. K.; Ogilby, P. R. Acc. Chem. Res. 2004, 37, 894-901.
15. eff, was 0.4. Luminescence from the sample was collected by the microscope objective and transmitted through a 1270 nm interference filter onto a near-IR photomultiplier tube (Hamamatsu model R5509-42, -80°C, rise time of 3 ns). For each laser pulse, data were recorded over two separate 50 μs sampling periods; the first began 1 μs after the laser pulse (signal channel) and the second began 500 μs after the laser pulse (background channel), and the difference between the two was obtained. For irradiation at a specific point in a given cell, the integration period for one acquisition was 10 s and was repeated 10 times to enhance the signal-to-noise ratio (i.e., at 1 kHz, each data set derives from 105 laser pulses). In the time-resolved experiment, the sampling period was reduced to 5 μs and counts were accumulated as the signal sampling window was successively delayed relative to the laser pulse.
16. eff = 1.4μm.
17. An important aspect of our pulsed-laser-based single photon counting experiments relative to steady state experiments is that we are able to temporally discriminate against comparatively intense and short-lived background luminescence from the sensitizer and optics that would otherwise interfere with the singlet oxygen signal.
18. Macpherson, A. N.; Truscott, T. G.; Turner, P. H. J. Chem. Soc., Faraday Trans. 1994, 90, 1065-1072.
19. Wessels, J. M.; Rodgers, M. A. J. J. Phys. Chem. 1995, 99, 17586-17592.
20. Wilkinson, F.; Helman, W. P.; Ross, A. B. J. Phys. Chem. Ref. Data 1995, 24, 663-1021.
21. Ogilby, P. R.; Foote, C. S. J. Am. Chem. Soc. 1983, 105, 3423-3430.
22. Moan, J. J. Photochem. Photobiol B, Biol. 1990, 6, 343-347.
23. Lee, P. C. C.; Rodgers, M. A. J. Photochem. Photobiol. 1987, 45, 79-86.
24. Lamond, A. I.; Sleeman, J. E. Curr. Biol. 2003, 13, 825-828.
25. Davila, J.; Harriman, A. Photochem. Photobiol. 1990, 57, 9-19.
26. Egorov, S. Y.; Kamalov, V. F.; Koroteev, N. I.; Krasnovsky, A. A.; Toleutaev, B. N.; Zinukov, S. V. Chem. Phys. Lett. 1989, 763, 421-424.
27. Schmidt, R.; Afshari, E. Ber. Bunsen-Ges. Phys. Chem. 1992, 96, 6, 788-794.
28. Tsushima, M.; Tokuda, K.; Ohsaka, T. Anal. Chem. 1994, 66, 4551-4556.