Total protein electrophoresis and RAPD fingerprinting analysis for the identification of aeromonas at the species level

Brazilian Journal of Microbiology

Volumn 33, Issue 4, 2002, Pages 358-362

  Longaray Delamare, Ana Paula ^a,b   De Oliveira Artico, Liane ^a   Grazziotin, Felipe Gobbi ^a   Echeverrigaray, Sergio ^a,c   Pinto Da Costa, Sérgio Olavo ^a,b,c  

^a UNIVERSITY OF CAXIAS DO SUL   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c Universidade Caxias do Sul   (Brazil)

Author keywords

Aeromonas; Molecular markers; Species identification

Indexed keywords

AEROMONAS;

EID: 19944377794     PISSN: 15178382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Altwegg, M.; Altwegg-Bissig, R.; Demarta, R.; Peduzzi, A.; Reeves, M.W.; Swaminathan, B. Comparison of four typing methods for Aeromonas species. J. Diarrh. Dis. Res., 6: 88-94, 1988.
2. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254, 1976.
3. Brune, W.; Alfenas, A.C. Identificação de proteínas em géis. In Alfenas, A.C. (Ed.) Eletroforese de isoenzimas e proteínas afins. Editora UFV, Viçosa, MG, 1998, p.183-197.
4. Carnaham, A.M.; Altwegg M. Taxonomy. In Austin, B.; Altwegg, M.; Gosling, P.J.; Joseph, S.W. (Ed.) The Genus Aeromonas. John Wiley & Sons Ltd., Chichester, England, 1995, p.1-38.
5. Collins, M.D.; Martinez-Murcia, A.J.; Cai, J. Aeromonas enteropelogenes and Aeromonas ichtiosmia are identical to Aeromonas trota and Areomonas veronii, respectively, as revealed by small-subunit rRNA sequence analysis. Int. J. Syst. Bacteriol., 43: 855-856, 1993.
6. Gosling, P.J. Aeromonas species in disease of animals. In Austin, B.; Altwegg, M.; Gosling, P.J.; Joseph, S.W. (Ed.) The Genus Aeromonas. John Wiley & Sons Ltd., Chichester, England, 1995, p.175-196.
7. Havelaar, A.H.; Schets, F.M.; Van Silfhout, A.; Janssen, W.H.; Wieten, G.; Van der Kooij, D. Typing of Aeromonas strains from patients with diarrhoea and from drinking water. J. Appl. Bacteriol., 72: 435-444, 1992.
8. Huys, G.; Coopman, R.; Janssen, P.; Kersters, K. High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int. J. Syst. Bacteriol., 46: 572-580, 1996.
9. Huys, G.; Kampfer, P.; Alwtwegg, M.; Coopman, R.; Janseen, P.; Gillis, M.; Kersters, K. Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia. Int. J. Syst. Bacteriol., 47: 1157-1164, 1997.
10. Janda, J.M.; Abbott, S.L. Human Pathogens. In Austin, B.; Altwegg, M.; Gosling, P.J.; Joseph, S.W. (Ed.) The Genus Aeromonas. John Wiley & Sons Ltd., Chichester, England, 1995, p.151-174.
11. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685, 1970.
12. Lee, J.V. Identification of Aeromonas in the routine laboratory. Experientia, 43: 355-356, 1987.
13. Martinez-Murcia, A.J.; Benlloch, S.; Collins, M.D. Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int. J. Syst. Bacteriol., 42: 412-421, 1992.
14. Millership, S.E.; Want, S.V. Characterization of strains of Aeromonas spp. by phenotype and whole-cell protein fingerprint. J. Med. Microbiol., 39: 107-113, 1993.
15. Muller, R.; Millership, S.E. Typing of Aeromonas spp. by numerical analysis of immunoblotted SDS-PAGE gels. J. Med. Microbiol., 39: 325-333, 1993.
16. Myata, M.; Aoki, T.; Inglis, V.; Yoshida, T.; Endo, M. RAPD analysis of Aeromonas salmonicida and Aeromonas hydrophila. J. Appl. Bacteriol., 79: 181-185, 1995.
17. Oakey, H.J.; Ellis, J.T.; Gibson, L.F. Differentiation of Aeromonas genomospecies using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). J. Appl. Bacteriol., 80: 402-410, 1996.
18. Oakey, H.J.; Ellis, J.T.; Gibson, L.F. The development of random DNA probes specific for Aeromonas salmonicida. J. Appl. Microbiol., 84: 37-46, 1998.
19. Oakey, H.J.; Gibson, L.F.; George, A.M. DNA probes specific for Aeromonas hydrophila. J. Appl. Microbiol., 86: 187-193, 1999.
20. Pan, Y.B.; Grisham, M.P.; Burner, D.M. A polymerase chain reaction protocol for the detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald disease. Plant Disease, 81: 189-194, 1997.
21. Picard, B.; Goullet, Ph. Comparative electrophoretic profiles of esterases, and of glutamate, lactate and malate deshydrogenases, from Aeromonas hydrophila, A. caviae and A. sobria. J. Gen. Microbiol., 131: 3385-3391, 1985.
22. Popoff, M.; Veron, M. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group. J. Gen. Microbiol., 94: 11-22, 1976.
23. Popoff, M.Y.; Coynault, C.; Kiredjan, M.; Lemelin, M. Polynucleotide sequence relatedness among motile Aeromonas species. Curr. Microbiol., 5: 109-114, 1981.
24. Popoff, M. Genus III. Aeromonas. In Krieg N.R.; Holt, J.G. (Ed.) Berger's Manual of Systematic Bacteriology, Vol. 1. Williams & Wilkins, Baltimore, MD, 1984, p.545-548.
25. Janda, J.M.; Abbott, S.L. Human Pathogens. In Austin, B.; Altwegg, M.; Gosling, P.J.; Joseph, S.W. (Ed.) The Genus Aeromonas. John Wiley & Sons Ltd., Chichester, England, 1995, p.151-174.
26. Rohlf, F.J. NTSYS-pc numerical taxonomy and multivariate analysis system, version 1.70. Exeter Software, Setauket, NY, 1989.
27. Shaw, D.; Hodder, H.J. Lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification. Can. J. Microbiol., 24: 864-868, 1978.
28. Stephenson, J.R.; Millership, S.E.; Tabaqchali, S. Typing of Aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins. J. Med. Microbiol., 24: 113-118, 1987.
29. Williams, J.G.K.; Kudelik, A.R.; Livak, K.J.; Rafalski, J.A. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 7213-7218, 1990.