Presence of the ica operon in clinical isolates of Staphylococcus epidermidis and its role in biofilm production

Clinical Microbiology and Infection

Volumn 10, Issue 12, 2004, Pages 1081-1088

  Cafiso, V ^a   Bertuccio, T ^a   Santagati, M ^a   Campanile, F ^a   Amicosante, G ^b   Perilli, M G ^b   Selan, L ^c   Artini, M ^c   Nicoletti, G ^a   Stefani, S ^a  

^a UNIVERSITY OF CATANIA   (Italy)

^b UNIVERSITY OF L'AQUILA   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Biofilm; Catheter associated infections; Coagulase negative staphylococci; ica operon; Staphylococcus epidermidis

Indexed keywords

AMPICILLIN; CIPROFLOXACIN; COTRIMOXAZOLE; ERYTHROMYCIN; GENTAMICIN; LEVOFLOXACIN; OXACILLIN; RIFAMPICIN; TEICOPLANIN; TETRACYCLINE; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIOFILM; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA SEQUENCE; GENE CLUSTER; GENE EXPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; STAPHYLOCOCCUS EPIDERMIDIS; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE;

STAPHYLOCOCCI; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 19944368738     PISSN: 1198743X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2004.01024.x     Document Type: Article

References (34)

1. Stefani S, Varaldo PE. Epidemiology of methicillin-resistant staphylococci in Europe. Clin Microbiol Infect 2003; 9: 1179-1186.
2. Vuong C, Otto M. Staphylococcus epidermidis infections. Microb Infect 2002; 4: 481-489.
3. Mack D, Fischer W, Krokotsch A et al. The intercellular adhesin involved in biofilm accumulation of Staphylococcus epidermidis is a linear β-1,6-linked glucosaminoglycan: purification and structural analysis. J Bacteriol 1996; 178: 175-183.
4. Götz F. Staphylococcus and biofilms. Mol Microbiol 2002; 43: 1367-1378.
5. Conlon KM, Humphreys H, O'Gara JP. icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis. J Bacteriol 2002; 184: 4400-4408.
6. Ziebuhr W, Krimmer V, Rachid S, Lößner I, Götz F, Hacker J. A novel mechanism of phase variation of virulence in Staphylococcus epidermidis: evidence for control of the polysaccharide intercellular adhesin synthesis by alternating insertion and excision of the insertion sequence element IS256. Mol Microbiol 1999; 32: 345-356.
7. Fey PD, Ulphani JS, Götz F, Heilmman C, Mack D, Rupp ME. Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in Staphylococcus epidermidis. J Infect Dis 1999; 179: 1561-1564.
8. Mack D, Riedewald J, Rohde H, Magnus T, Feucht HH, Elsner HA. Essential functional role of the polysaccharide intercellular adhesin of Staphylococcus epidermidis in hemagglutination. Infect Immun 1999; 67: 1004-1008.
9. Heilmman C, Schweitzer O, Gerke C, Vanittanakom N, Mack D, Götz F. Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol 1996; 20: 1083-1091.
10. Cramton SE, Ulrich M, Götz F, Döring G. Anaerobic conditions induce expression of polysaccharide intercellular adhesin in Staphylococcus aureus and Staphylococcus epidermidis. Infect Immun 2001; 69: 4079-4085.
11. Knobloch JKM, Horstkotte MA, Rohde H, Kaulfers PM, Mack D. Alcoholic ingredients in skin disinfectants increase biofilm expression of Staphylococcus epidermidis. J Antimicrob Chemother 2002; 49 683-687.
12. B is involved in regulation of biofilm expression in a Staphylococcus aureus mucosal isolate. J Bacteriol 2000; 182: 6824-6826.
13. Donelli G, De Paoli P, Fadda G et al. A multicenter study on central venous catheter-associated infections in Italy. J Chemother 2001; 13(suppl 1): 251-262.
14. Mermel LA, Farr BM, Sherertz RJ et al. Guidelines for the management of intravascular catheter-related infections. Clin Infect Dis 2001; 32: 1249-1272.
15. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility testing for bacteria that grow aerobically, 6th edn. Approved standard M7-A6. Wayne, PA: NCCLS, 2003.
16. Hussain M, Hastings JG, White PJ. A chemically defined medium for slime production by coagulase-negative staphylococci. J Med Microbiol 1991; 34: 143-147.
17. Freeman DJ, Falkiner F, Keane CT. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 1989; 42: 872-874.
18. Pitcher DG, Sanders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151-156.
19. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977; 74 5463-5467.
20. Enright MC, Day NPJ, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 1999; 38: 1008-1015.
21. Cafiso V, Campanile F, Borbone S et al. Correlation between methicillin-resistance and resistance to fluoroquinolones in Staphylococcus aureus and Staphylococcus epidermidis. Infect Med 2001; 2: 90-97.
22. de Lancaster H, Couto I, Sanches IS, Melo-Oristino Pereira AT, Thomasz A. Methicillin-resistance in Staphylococcus aureus disease in a Portuguese hospital: characterization of clonal type by a combination of DNA typing methods. Eur J Clin Microbiol Infect Dis 1994; 13: 64-73.
23. Tenover FC, Arbeit R, Goering RV et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-2239.
24. Struelens MJ, Deplano A, Godard C, Maes N, Serruys E. Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis. J Clin Microbiol 1992; 30: 2599-2605.
25. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979; 76: 5269-5273.
26. Christensen GD, Simpson WA, Younger JJ et al. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 1985; 22: 996-1006.
27. Raad I, Alrahwan A, Roloston K. Staphylococcus epidermidis emerging resistance and need for alternative agents. Clin Infect Dis 1998; 26: 1182-1187.
28. Frebourg NB, Lefebvre S, Baert S, Lemeland JF. PCR-based assay for discrimination between invasive and contaminating Staphylococcus epidermidis strains. J Clin Microbiol 2000; 38 877-880.
29. Ziebuhr W, Heilman C, Götz F et al. Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infect Immun 1997; 65: 890-896.
30. De Silva GDI, Kantzanou M, Massey RC, Wilkinson AR, Day NPJ, Peacock SJ. The ica-operon and biofilm production in coagulase-negative staphylococci associated with carriage and disease in a neonatal intensive care unit. J Clin Microbiol 2002; 40: 382-388.
31. Patti JM, Allen BL, McGavin MJ, Hook M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol 1994; 48: 585-617.
32. Montanaro LC, Arciola CR, Borsetti E, Brigotti M, Baldassari L. A polymerase chain reaction (PCR) method for the identification of collagen adhesin gene (cna) in Staphylococcus-induced prosthesis infections. New Microbiol 1998; 21: 359-363.
33. Montanaro LC, Arciola CR, Borsetti E, Collamati S, Baldassarri L, Montanaro L. Detection of fibronectin-binding protein genes in staphylococcal strains from periprosthesis infections. New Microbiol 1999; 22: 331-336.
34. Montanaro LC, Arciola CR, Baldassarri L, Borsetti E. Presence and expression of collagen adhesin gene (cna) and slime production in Staphylococcus aureus strains from orthopedic prosthesis infections. Biomaterials 1999; 20: 1945-1949.