PCR detection of four periodontopathogens from subgingival clinical samples

Brazilian Journal of Microbiology

Volumn 34, Issue 1, 2003, Pages 81-84

  Avila Campos, Mario Julio ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

PCR; Periodontal disease; Putative periodontal pathogens

Indexed keywords

BACTERIA (MICROORGANISMS);

EID: 19944362232     PISSN: 15178382     EISSN: None     Source Type: Journal    
DOI: 10.1590/S1517-83822003000100017     Document Type: Article

References (12)

1. Ashimoto, A.; Chen, C.; Bakker, I.; Slots, J. Polymerase chain reaction detection of 8 putative penodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol. Immunol., 11: 266-273, 1996 .
2. Avila-Campos, M.J.; Farias, L.M.; Carvalho, M.A.R.; Damascene, C.A.V.; Cisalpino, E.O.; Costa, J.E. Aspectos ecológicos de Actinobacillus actinomycetemcomitans: aislamiento y caracterización de cepas. Rev. Lat-amer. Microbiol., 30: 301-305, 1988.
3. Avila-Campos, M.J.; Sacchi, C.T.; Whitney, A.M.; Steigerwalt, A.G.; Mayer, L.W. Arbitrary primed-polymerase chain reaction for identification and epidemiologic subtyping of oral isolates of Fusobacterium nucleatum. J. Periodontol., 70: 1202-1208, 1999.
4. Bolstad, A.I.; Jensen, H.B. Polymerase chain reaction-amplified nonradioactive probes for identification of Fusobacterium nucleatum. J. Clin. Microbiol., 31: 528-532, 1993.
5. Eisenstein, B.I. New molecular techniques for microbial epidemiology and the diagnosis of infectious diseases. J. Infect. Dis., 161: 595-602, 1990.
6. Furcht, C.; Eschrich, K.; Merte, K. Detection of Eikenella corrodens and Actinobacillus actinomycetemcomitans by use of the polymerase chain reaction (PCR) in vitro and in subgingival plaque. J. Clin. Periodontol., 23: 891-897, 1996.
7. Ménard, C.; Brousseau, R.; Mouton, C. Application of polymerase chain reaction with arbitrary primer (AP-PCR) to strain identification of Porphyromonas (Bacteroides) gingivalis. FEMS Microbiol. Lett., 95: 163-168, 1992.
8. Möller, A.J.R. Microbiological examination of root canals and periapical tisues of human teeth. Odontologisk Tidskrift., 74: 1-380, 1966.
9. Newman, M.G.; Socransky, S.S.; Savitt, E.; Propas, D.; Crawford, A.A. Studies of the microbiology of periodontitis. J. Periodontol, 47: 373-379, 1976.
10. Newman, M.G.; Socransky, S.S. Predominant cultivable microbiota in penodontitis. J. Periodontol. Res., 12: 120-128, 1977.
11. Slots, J.; Ashimoto, A.; Flynn, M.J.; Li, G.; Chen, C. Detection of putative pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin. Infect. Dis., 20: 304-307, 1995.
12. Watanabe, K.; Frommel, T. O. Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola detection in oral plaque samples using the polymerase chain reaction. J. Clin. Periodontol, 23: 212-219, 1996.