Characterization of fluorescent chimeras of cholera toxin and Escherichia coli heat-labile enterotoxins produced by use of the twin arginine translocation system

Infection and Immunity

Volumn 73, Issue 6, 2005, Pages 3627-3635

  Tinker, Juliette K ^a,c   Erbe, Jarrod L ^b   Holmes, Randall K ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b Wisconsin Lutheran College   (United States)

^c BOISE STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; ARGININE; CHLORAMPHENICOL; CHOLERA TOXIN; ENTEROTOXIN; ESCHERICHIA COLI ENTEROTOXIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; POLYPEPTIDE; RED FLUORESCENT PROTEIN; VACCINE;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; BINDING SITE; CHIMERA; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RECEPTOR BINDING; TOXICITY; VERO CELL; WESTERN BLOTTING;

ANIMALS; BACTERIAL TOXINS; BIOLOGICAL TRANSPORT; CERCOPITHECUS AETHIOPS; CHOLERA TOXIN; ENTEROTOXINS; ESCHERICHIA COLI PROTEINS; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; MEMBRANE TRANSPORT PROTEINS; RECOMBINANT FUSION PROTEINS; VERO CELLS;

EID: 19744375740     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.73.6.3627-3635.2005     Document Type: Article

References (46)

1. Bastiaens, P., I. Majoul, P. Verveer, H. Söling, and T. Jovin. 1996. Imaging the intracellular trafficking and state of the AB5 quaternary structure of cholera toxin. EMBO J. 15:4246-4253.
2. Behrendt, J., K. Standar, U. Lindenstraub, and T. Bruser. 2004. Topological studies on the twin-translocase component TatC. FEMS Microbiol. Lett. 234:303-308.
3. Campbell, R. E., O. Tour, A. E. Palmer, P. A. Steinbach, G. S. Baird, D. A. Zacharias, and R. Y. Tsien. 2002. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99:7877-7882.
4. Chen, A., T. Hu, C. Mikoryak, and R. Draper. 2002. Retrograde transport of protein toxins under conditions of COPI dysfunction. Biochim. Biophys. Acta 1589:124-139.
5. De Haan, L., and T. R. Hirst. 2002. Bacterial toxins as versatile delivery vehicles. Curr. Opin. Drug Devel. 5:269-278.
6. De Haan, L., and T. R. Hirst. 2004. Cholera toxin: a paradigm for multifunctional engagement of cellular mechanisms. Mol. Membr. Biol. 21:77-92.
7. Fujinaga, Y., A. Wolf, C. Rodighiero, H. Wheeler, B. Tsai, L. Allen, M. Jobling, T. Rapoport, R. Holmes, and W. Lencer. 2003. Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulum. Mol. Biol. Cell 14:4783-4793.
8. George-Chandy, A., K. Eriksson, M. Lebens, I. Nordstrom, E. Schon, and J. Holmgren. 2001. Cholera toxin B subunit as a carrier molecule promotes antigen presentation and increases CD40 and CD86 expression of antigen-presenting cells. Infect. Immun. 69:5716-5725.
9. Hajishengallis, G., E. Harokopakis, S. Hollingshead, M. Russell, and S. Michalek. 1996. Construction and oral immunogenicity of a Salmonella typhimurium strain expressing a streptococcal adhesion linked to the A2/B subunits of cholera toxin. Vaccine 14:1545-1548.
10. Hajishengallis, S. Hollingshead, T. Koga, and M. Russell. 1995. Mucosal immunization with a bacterial protein antigen genetically coupled to cholera toxin A2/B subunits. J. Immunol. 22:4322-4433.
11. Hardy, S. J. S., J. Holmgren, S. Johansson, J. Sanchez, and T. R. Hirst. 1988. Coordinated assembly of multisubunit proteins: oligomerization of bacterial enterotoxins in vivo and in vitro. Proc. Natl. Acad. Sci. USA 85:7109-7113.
12. Hatic, S. O., J. A. McCann, and W. D. Picking. 2001. In vitro assembly of novel cholera toxin-like complexes. Anal. Biochem. 292:171-177.
13. Hazes, B., and R. Read. 1997. Accumulating evidence suggests that several AB-toxins subvert the endoplasmic reticulum-associated protein degradation pathway to enter target cells. Biochemistry 36:11051-11054.
14. Hirst, T. R. 2001. A toxin with emerging therapeutic potential. Lancet 358(Suppl.):S7.
15. Holmes, R. K., and E. M. Twiddy. 1983. Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits. Infect. Immun. 42:914-923.
16. Ize, B., F. Gerard, M. Zhang, A. Chanal, R. Voulhoux, T. Palmer, A. Filloux, and L. Wu. 2002. In vivo dissection of the tat translocation pathway in Escherichia coli. J. Mol. Biol. 317:327-335.
17. Jobling, M. G., and R. K. Holmes. 1992. Fusion proteins containing the A2 domain of cholera toxin assemble with B polypeptides of cholera toxin to form immunoreactive and functional holotoxin-like chimeras. Infect. Immun. 60:4915-4924.
18. Jobling, M. G., L. M. Palmer, J. L. Erbe, and R. K. Holmes. 1997. Construction and characterization of versatile cloning vectors for efficient delivery of native foreign proteins to the periplasm of Escherichia coli. Plasmid 38:158-173.
19. Kim, B, S. Shin, Y. Yoo, and S. Pyo. 2001. Peroral immunization with Helicobacter pylori adhesion protein genetically linked to cholera toxin A2B subunits. Clin. Sci. 100:291-298.
20. Kweon, M.-N., M. Yamamoto, F. Watanabe, S. Tamura, F. W. van Ginkel, A. Miyauchi, H. Takagi, Y. Takeda, T. Hamabata, K. Fujihashi, J. R. McGee, and H. Kiyono. 2002. A nontoxic chimeric enterotoxin adjuvant induces protective immunity in both mucosal and systemic compartments with reduced IgE antibodies. J. Infect. Dis. 186:1261-1269.
21. Lauer, S., B. Goldstein, R. L. Nolan, and J. P. Nolan. 2002. Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry 41: 1742-1751.
22. Lee, S., S. Halperin, D. Salloum, A. MacMillan, and A. Morris. 2003. Mucosal immunization with a genetically engineered pertussis toxin S1 fragment-cholera toxin subunit A chimeric protein. Infect. Immun. 71:2272-2275.
23. Lencer, W. I., C. Constable, S. Moe, P. A. Rufo, A. Wolf, M. G. Jobling, S. Ruston, J. L. Madara, R. K. Holmes, and T. R. Hirst 1997. Proteolytic activation of cholera toxin and Escherichia coli labile toxin by entry into host epithelial cells. Signal transduction by a protease-resistant variant. J. Biol. Chem. 272:15562-15568.
24. Lencer, W. I., C. Constable, S. Moe, M. G. Jobling, H. M. Webb, S. Ruston, J. L. Madara, T. R. Hirst, and R. K. Holmes. 1995. Targeting of cholera toxin and Escherichia coli heat-labile toxin to polarized epithelia: role of COOH-terminal KDEL. J. Cell Biol. 131:951-962.
25. Lencer, W. I., T. R. Hirst, and R. K. Holmes. 1999. Membrane traffic and the cellular uptake of cholera toxin. Biochim. Biophys. Acta 1450:177-190.
26. Leong, J., A. C. Vinal, and W. S. Dallas. 1985. Nucleotide sequence comparison between heat-labile toxin B-subunit cistrons from Escherichia coli of human and porcine origin. Infect. Immun. 48:73-77.
27. Li, X., J. L. Erbe, C. V. Lockatell, D. E. Johnson, M. G. Jobling, R. K. Holmes, and H. L. Mobley. 2004. Use of a translational fusion of the MrpH fimbrial adhesion binding domain with cholera toxin A2 domain, coexpressed with the cholera toxin B subunit, as an intranasal vaccine to prevent experimental urinary tract infection by Proteus mirabilis. Infect. Immun. 72:7306-7310.
28. Majoul, I., P. Bastiaens, and H. Söling. 1996. Transport of an external Lys-Asp-Glu-Leu (KDEL) protein from the plasma membrane to the endoplasmic reticulum: studies with cholera toxin in vero cells. J. Cell Biol. 133:777-789.
29. Mamdouh, Z., M. C. Giocondi, R. Laprade, and C. Le Grimellec. 1996. Temperature dependence of endocytosis in renal epithelial cells culture. Biochim. Biophys. Acta 1282:171-173.
30. + T cells. Infect. Immun. 69:252-261.
31. + T cells. Infect. Immun. 69:4486-4492.
32. Middlebrook, J., and R. Dorland. 1984. Bacterial toxins: cellular mechanisms of action. Microbiol. Rev. 48:199-221.
33. Salmond, R. J., J. A. Luross, and N. A. Williams. 2002. Immune modulation by the cholera-like enterotoxins. Expert Rev. Mol. Med. 2002:1-16.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
35. Sanchez, J., G. Wallerstrom, M. Fredriksson, J. Angstrom, and J. Holmgren. 2002. Detoxicification of cholera toxin without removal of its immunoadjuvanticity by the addition of (STa-related) peptides to the catalytic subunit: a potential new strategy to generate immunostimulants for vaccination. J. Biol. Chem. 277:33369-33377.
36. Santini, C. A. Bernadac, M. Zhang, A. Chanal, B. Ize, C. Blanco, and L. Wu. 2001. Translocation of jellyfish green fluorescent protein via the Tat system of Escherichla coli and change of its periplasmic localization in response to osmotic up-shock. J. Biol. Chem. 276:8159-8164.
37. Sargent, F., U. Gohlke, E. de Leeuw, N. R. Stanley, T. Palmer, H. R. Saibil, and B. Berks. 2001. Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure. Eur. J. Biochem. 268:3361-3367.
38. Spence, E., M. Sarcina, N. Ray, S. Geir Moller, C. W. Mullineaux, and C. Robinson. 2003. Membrane-specific targeting of green fluorescent protein by the Tat pathway in the cyanobacterium Synechocystis PCC6803. Mol. Microbiol. 48:1481-1489.
39. 2 domain of cholera toxin. Infect. Immun. 66:462-468.
40. Tartof, K. D., and C. A. Hobbs. 1987. Improved media for growing plasmid and cosmid clones. Focus 9:12.
41. Thomas, J. D., R. A. Daniel, J. Errington, and C. Robinson. 2001. Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli. Mol. Microbiol. 39:47-53.
42. Tinker, J. K., J. L. Erbe, W. G. Hol, and R. K. Holmes. 2003. Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system. Infect. Immun. 71:4093-4101.
43. Toida, N., G. Hajishengallis, H. Wu, and M. Russell. 1997. Oral immunization with the saliva-binding region of Streptococcus mutons AgI/II genetically coupled to the cholera toxin B subunit elicits T-helper cell responses in gut-associated lymphoid tissues. Infect. Immun. 65:909-915.
44. Weiner, J. H., P. T. Bilous, G. M. Shaw, S. P. Lubitz, L. Frost, G. H. Thomas, J. A. Cole, and R. J. Turner. 1998. A novel and ubiquitous system for membrane targeting and secretion of cofactor-containing proteins. Cell 93: 93-101.
45. Yahr, T., and W. T. Wickner. 2001. Functional reconstruction of bacterial Tat translocation in vitro. EMBO J. 20:2472-2479.
46. Yu, J., and W. Langrudge. 2001. A plant-based multicomponent vaccine protects mice from enteric diseases. Nat. Biotechnol. 19:548-552.