A simple technique for localizing consecutive fields for disector pairs in light microscopy: Application to neuron counting in rabbit spinal cord following spinal cord injury

Journal of Neuroscience Methods

Volumn 145, Issue 1-2, 2005, Pages 277-284

  Kaplan, Süleyman ^a   Gökyar, Ahmet ^b   Ünal, Bünyami ^c   Tunç, Ayten Türkkani ^d   Bahadir, Ayşe ^a   Aslan, Hüseyin ^d  

^a ONDOKUZ MAYIS UNIVERSITY   (Turkey)

^b Neurosurgery Clinic   (Turkey)

^c ATATURK UNIVERSITY   (Turkey)

^d GAZIOSMANPASA UNIVERSITY   (Turkey)

Author keywords

Hypothermia; Injury; Methylprednisolone; Physical fractionator; Spinal cord; Stereology

Indexed keywords

METHYLPREDNISOLONE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; INDUCED HYPOTHERMIA; MICROSCOPE; MICROSCOPY; NEUROPROTECTION; NONHUMAN; PRIORITY JOURNAL; RABBIT; RANDOMIZATION; SPINAL CORD INJURY; STEREOLOGY; THORACIC SPINAL CORD; TISSUE SECTION;

ANIMALS; MICROSCOPY; NEURONS; RABBITS; SPINAL CORD INJURIES;

EID: 19544367122     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.02.012     Document Type: Article

References (44)

1. B. Battiston, P. Tos, S. Geuna, M.G. Giacobini-Robecchi, and R. Guglielmone Nerve repair by means of vein filled with muscle grafts II. Morphological analysis of regeneration Microsurgery 20 2000 37 41
2. C.V. Borlongan, G. Yu, N. Matsukawa, L. Xu, D.C. Hess, and P.R. Sanberg Acute functional effects of cyclosporine-A and methylprednisolone treatment in adult rats exposed to transient ischemic stroke Life Sci 76 2005 1503 1512
3. S. Bradbury Linear measurement with the light microscope Microsc Anal 17 1990 7 12
4. H. Brændgaard, and H.J.G. Gundersen The impact of recent stereological advances on quantitative studies of the nervous system J Neurosci Methods 18 1986 39 79
5. J.Y. Delattre, E. Arbit, H.T. Thaler, M.K. Rosenblum, and J.B. Posner A dose-response study of dexamethasone in a model of spinal cord compression caused by epidural tumor J Neurosurg 70 1989 920 925
6. J.R. Glaser, and E.M. Glaser Stereology, morphometry, and mapping: the whole is greater than the sum of its parts J Chem Neuroanat 20 2000 115 126
7. A. Gok, C. Uk, M. Yilmaz, K. Bakir, I. Erkutlu, and M. Alptekin Efficacy of methylprednisolone in acute experimental cauda equine injury Acta Neurochir (Wien) 144 2002 817 821
8. Q.Y. Gong, J.N. Brunt, C.S. Romaniuk, J.P. Oakley, L.T. Tan, and N. Roberts Contrast enhanced dynamic MRI of cervical carcinoma during radiotherapy: early prediction of tumour regression rate Br J Radiol 72 1999 1177 1184
9. M.J. Graves, and D.M. Dommett Comparison of cardiac stroke volume measurement determined using stereological analysis of breath-hold cine MRI and phase contrast velocity mapping Br J Radiol 73 2000 825 832
10. H.J.G. Gundersen Notes on the estimation of the numerical density of arbitrary particles: the edge effect J Microsc 111 1977 219 223
11. H.J.G. Gundersen Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones in memory of William R Thomson J Microsc 143 1986 3 45
12. J.G. Gundersen, P. Bagger, T.F. Bendtsen, S.M. Evans, L. Korbo, and N. Marcussen The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis Acta Pathol Microbiol Immunol Scand 96 1988 857 881
13. H.J.G. Gundersen, and E.B. Jensen The efficiency of systematic sampling in stereology and its prediction J Microsc 147 1987 229 263
14. H.J.G. Gundersen, E.B.V. Jensen, K. Kiêu, and J. Nielsen The efficiency of systematic sampling in stereology-reconsidered J Microsc 193 1999 199 211
15. T. Gunnarsson, and M.G. Fehlings Acute neurosurgical management of traumatic brain injury and spinal cord injury Curr Opin Neurol 16 2003 717 723
16. O. Guntinas-Lichius, J. Mockenhaupt, E. Stennert, and W.F. Neiss Simplified nerve cell counting in the rat brainstem with the physical disector using a drawing-microscope J Microsc 172 1993 177 180
17. C.V. Howard, and M.G. Reed Unbiased stereology. In: Three-dimensional measurement in microscopy 1998 Bios Scientific Publisher Guilford, UK p. 187-95
18. J. Inamasu, Y. Nakamura, and K. Ichikizaki Induced hypothermia in experimental traumatic spinal cord injury: an update J Neurol Sci 209 2003 55 60
19. S. Kaplan, S. Canan, H. Aslan, B. Unal, and B. Sahin A simple technique to measure the movements of the microscope stage along the x and y axes for stereological methods J Microsc 203 2001 321 325
20. D. Kim, T.A. Jahng, and L. Li Combination therapy with methylprednisolone and brain-derived neurotrophic factor for acute spinal cord injury Spine J 2 Suppl 2002 34
21. B.K. Kwon, W. Tetzlaff, J.N. Grauer, J. Beiner, and A.R. Vaccoro Pathophysiology and pharmacologic treatment of acute spinal cord injury Spine J 4 2004 451 464
22. J.O. Larsen Stereology of nerve cross sections J Neurosci Methods 85 1998 107 118
23. C.E. Mackay, B. Pakkenberg, and N. Roberts Comparison of compartment volumes estimated from MR images and physical sections of formalin fixed cerebral hemispheres Acta Stereol 18 1999 149 159
24. D.W. Marion, L.E. Penrod, S.F. Kelsey, W.D. Obrist, P.M. Kochanek, and A.M. Palmer Treatment of traumatic brain injury with moderate hypothermia N Engl J Med 336 1997 540 546
25. E.P. McCutcheon, A.W. Selassie, J.K. Gu, and E.E. Pickelsimer Acute traumatic cord injury, 1993-2000. A population based assessment of methylprednisolone administration and hospitalisation J Trauma 56 2004 1076 1083
26. E.D. Means, D.K. Anderson, T.R. Waters, and L. Kalaf Effect of methylprednisolone in compression trauma to the feline spinal cord J Neurosurg 55 1981 200 208
27. A. Merola, M.F. O'Brien, B.A. Castro, D.A. Smith, J.M. Eule, and T.G. Lowe Histologic characterization of acute spinal cord injury treated with intravenous methylprednisolone J Orthop Trauma 16 2002 155 161
28. A. Møller, P. Strange, and H.J.G. Gundersen Efficient estimation of cell volume and number using the nucleator and the disector J Microsc 159 1990 61 71
29. T.G. Ohm, C. Busch, and J. Bohl Unbiased estimation of neuronal numbers in the human nucleus coeruleus during aging Neurobiol Aging 18 1997 393 399
30. C.M. Pover, and R.E. Coggeshall Verification of the disector method for counting neurons, with comments on the empirical method Anat Rec 231 1991 573 578
31. C. Schmitz, H. Korr, D.P. Perl, and P.R. Hof Advanced use of 3-D methods for counting neurons Trends Neurosci 24 2001 377 380
32. A. Sharma, R. Tiwari, P. Badhe, and G. Sharma Comparison of methylprednisolone with dexamethasone in treatment of acute spinal injury in rats Indian J Exp Biol 42 2004 476 480
33. O.F. Sonmez, B. Unal, S. Inaloz, B. Sahin, M. Yilmaz, and A. Aydin Therapeutic effects of intracarotid infusion of spermine/nitric oxide complex on cerebral vasospasm Acta Neurochir (Wien) 144 2002 921 928
34. D.C. Sterio The unbiased estimation of number and sizes of arbitrary particles using the disector J Microsc 134 1984 127 136
35. Y. Taoka, and K. Okajima Spinal cord injury in the rat Prog Neurobiol 56 1998 341 358
36. C.H. Tator, and I. Koyanagi Vascular mechanisms in the pathophysiology of human spinal cord injury J Neurosurg 86 1997 483 492
37. P. Tos, B. Battiston, S. Geuna, M.G. Giacobini-Robecchi, M.A. Hill, M. Lanzetta, and E.R. Owen Tissue specificity in rat peripheral nerve regeneration through combined skeletal muscle and vein conduit grafts Microsurgery 20 2000 65 71
38. J.H. Tucker, R. Dye, J. Sprey, C. Sowter, E. Gray, and G. Brugal Relocation accuracy on HOME computerized microscopes J Microsc 176 1994 75 82
39. I. Vanicky, M. Marsala, J. Galik, and J. Marsala Epidural perfusion cooling protection against protracted spinal cord ischemia in rabbits J Neurosurg 79 1993 736 741
40. S.D. Weirich, H.B. Cotler, P.A. Narayana, J.D. Hazle, E.F. Jackson, and K.J. Coupe Histopathologic correlation of magnetic resonance imaging signal patterns in a spinal cord injury model Spine 15 1990 630 638
41. R.J. White, and M. Albin Spinal cord cooling J Neurosurg Spine 94 1 Suppl 2001 183 184
42. G. Yu, D.C. Hess, and C.V. Borlongan Combined cyclosporine-A and methylprednisolone treatment exerts partial and transient neuroprotection against ischemic stroke Brain Res 1018 2004 32 37
43. W.R. Yu, H. Westergren, M. Farooque, A. Holtz, and Y. Olsson Systemic hypothermia following spinal cord compression injury in the rat: an immunohistochemical study on MAP 2 with special reference to dendrite changes Acta Neuropathol (Berl) 100 2000 546 552
44. S.M. Zeidman, G.S. Ling, T.B. Ducker, and R.G. Ellenbogen Clinical applications of pharmacologic therapies for spinal cord injury J Spinal Disord 9 1996 367 380