A spectrophotometric method for estimating hemin in biological systems

Analytical Biochemistry

Volumn 341, Issue 2, 2005, Pages 199-203

  Lombardo, María Elisa ^a   Araujo, Lidia Susana ^a   Ciccarelli, Alejandra Beatriz ^a   Batlle, Alcira ^a,b  

^a Centro de Investigaciones sobre Porfirinas y Porfirias   (Argentina)

^b NONE   (Argentina)

Author keywords

Hemin extraction; Hemin quantification; Pyridine hemochromogen method; S' Soret band

Indexed keywords

BIOLOGICAL MATERIALS; CHLORINE COMPOUNDS; PYRIDINE; SPECTROPHOTOMETERS;

ABSORPTION MAXIMA; CHLOROFORM EXTRACTS; CORRECTION FORMULAS; HEMIN QUANTIFICATION; LINEAR CORRELATION; SORET BANDS; SPECTRO-PHOTOMETRIC METHOD; SPECTROPHOTOMETRIC ASSAYS;

SPECTROPHOTOMETRY;

CHLOROFORM; CHROMOGENIC SUBSTRATE; HEMIN; PORPHYRIN; PYRIDINE;

ABSORPTION; ACIDITY; AMINO ACID ANALYSIS; ARTICLE; CORRELATION ANALYSIS; EXTRACT; EXTRACTION; LINEAR SYSTEM; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RECORDING; REPRODUCIBILITY; SENSITIVITY ANALYSIS; SPECTROPHOTOMETRY;

EID: 19444387311     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.11.002     Document Type: Article

References (11)

1. A.M. del, C. Batlle, Biosíntesis del Hemo, in: Porfirias y Porfirinas. Aspectos Clínicos, bioquímicos y biologí a molecular, Acta Bioquím. Clín. Latinoamericana Sup. 3, Argentina, 1997
2. J.K. Pal, and M. Joshi-Purandare Dose-dependent differential effect of hemin on protein synthesis and cell proliferation in Leishmania donovani promastigotes cultured in vitro J. Biosci. 26 2001 225 231
3. J.-J. Chen, and I.M. London Regulation of protein synthesis by heme-regulated eIF-2α kinase Trends Biochem. Sci. 20 1995 105 108
4. J.K. Pal, S. Anand, and J. Joseph Association of Hsp90 with the heme-regulated eukaryotic initiation factor 2α kinase-a collaboration for regulating protein synthesis J. Biosci. 21 1996 191 205
5. C.S. Chang, and K.P. Chang Heme requirement and acquisition by extracellular and intracellular stages of Leishmania mexicana amazoneusis Mol. Biochem. Parasitol. 16 1985 267 276
6. J. Bauer, and J. Fornnarino High-performance liquid chromatographic analysis of "available" hemin in hematin solutions J. Chromatogr. 283 1984 378 382
7. C.A. Genco, B.M. Odusanya, and G. Brown Binding and accumulation of hemin in Porphyromonas gingivalis are induced by hemin Infect. Inmmun. 62 1994 2885 2892
8. R.J. Porra, and O.T.G. Jones Studies on ferrochelatase. 1. Assay and properties of ferrochelatase from a pig-liver mitochondrial extract Biochem. J. 87 1963 181 185
9. P.S. Clezy, and D.B. Morell A spectroscopic study of haematin compounds in the Soret region Biochim. Biophys. Acta 71 1963 150 156
10. M.E. Lombardo, L.S. Araujo, and A. Batlle 5-Aminolevulinic acid synthesis in epimastigotes of Trypanosoma cruzi Int. J. Biochem. Cell Biol. 35 2003 1263 1271
11. M.E. Lombardo, L.S. Araujo, A. Branca, A.M. del, and C. Batlle A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde Z. Naturforsch. C 45 1990 911 914