The epiplasm gene EPC1 influences cell shape and cortical pattern in Tetrahymena thermophila

Journal of Eukaryotic Microbiology

Volumn 51, Issue 2, 2004, Pages 201-206

  Williams, Norman E ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

Conjugation; Cortical development; EPCI; Epiplasm; Gene knockout; Membrane skeleton; Tetrahymena

Indexed keywords

PROTOZOAL DNA; PROTOZOAL PROTEIN;

GENETIC ANALYSIS;

5' UNTRANSLATED REGION; ANIMAL; ARTICLE; BACTERIUM CONJUGATION; CHEMISTRY; CYTOLOGY; CYTOSKELETON; DNA SEQUENCE; GENE; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC TRANSFORMATION; GENETICS; INTRON; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHENOTYPE; PHYSIOLOGY; SEQUENCE HOMOLOGY; TETRAHYMENA THERMOPHILA;

5' UNTRANSLATED REGIONS; ANIMALS; CONJUGATION, GENETIC; CYTOSKELETON; DNA, PROTOZOAN; GENE DELETION; GENES, PROTOZOAN; INTRONS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OPEN READING FRAMES; PHENOTYPE; PROTOZOAN PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY; TETRAHYMENA THERMOPHILA; TRANSFORMATION, GENETIC;

ANIMALIA; BACTERIA (MICROORGANISMS); PROTOZOA; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 1942537185     PISSN: 10665234     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2004.tb00546.x     Document Type: Article

References (24)

1. Aufderheide, K. 1983. Mitochondrial associations with specific components of the cortex of Tetrahymena: microtubules and the epiplasm. J. Protozool., 30:457-460.
2. Bouchard, P., Chomilier, J., Ravet, V., Mornon, J-P. & Viguěs, B. 2001. Molecular characterization of the major membrane skeletal protein in the ciliate Tetrahymena pyriformis suggests n-plication of an early evolutionary intermediate filament protein subdomain. J. Cell Sci., 114:101-110.
3. Cassidy-Hanley, D., Bowen, J. Lee, J., Cole, E. S., VerPlank, J., Gaertig, M. A., Gorovsky, M. & Bruns, P. J. 1997. Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics, 146:135-147.
4. Dress, V., Yi, H., Musel, M. R. & Williams, N. E. 1992. Tetrin polypeptides are colocalized in the cortex of Tetrahymena. J. Struct. Biol., 108:187-194.
5. Dubreuil, R. R. & Bouck, G. B. 1998. Interrelationships among the plasma membrane, the membrane skeleton and surface form in a unicellular flagellate. Protoplasma, 143:150-164.
6. Elliott, A. M. & Tremor, J. W. 1958. The fine structure of the pellicle in the contact area of conjugating Tetrahymena pyriformis. J. Biophys. Biochem. Cytol., 4:839-840.
7. Frankel, J. & Nelsen, E. M. 2001. The effects of supraoptimal temperatures on population growth and cortical patterning in Tetrahymena pyriformis and Tetrahymena thermophila: A comparison. J. Eukaryot. Microbiol., 48:135-146.
8. Frankel, J. Williams, N. E., Nelsen, E. M. & Keeling, P. J. 2001. An evaluation of Hsp90 as a mediator of cortical patterning in Tetrahymena. J. Eukaryot. Microbiol., 48:147-160.
9. Hamilton, E. P. & Orias, E. 1999. Genetic crosses: setting up crosses, testing progeny, and isolating phenotypic assortants. In: Asai, D. J. & Forney, J. D. (ed.), Methods in Cell Biology: Tetrahymena thermophila. Academic Press, San Diego, CA. 62:189-211.
10. Honts, J. E. & Williams, N. E. 2003. Novel cytoskeletal proteins in the cortex of Tetrahymena. J. Eukaryot. Microbiol., 50:9-14.
11. Huttenlauch, I., Peck, R. K. & Stick, R. 1998. Articulins and epiplasmins: two distinct classes of cytoskeletal proteins of the membrane skeleton in protists. J. Cell Sci., 111:3367-3378.
12. Kaczanowska, J. Buzanska, L. & Ostrowski, M. 1993. Relationships between spatial pattern of basal bodies and membrane skeleton (epiplasm) during the cell cycle of Tetrahymena: cdaA mutant and antimembrane skeleton immunostaining. J. Eukaryot. Microbiol., 40: 747-754.
13. McDonald, B. B. 1966. The exchange of RNA and protein during conjugation in Tetrahymena. J. Protozool., 13:277-285.
14. Nelsen, E. M., Williams, N. E., Yi, H., Knaak, J. & Frankel, J. 1994. "Fenestrin" and conjugation in Tetrahymena thermophila. J. Eukaryot. Microbiol., 41:483-495.
15. Orias, E. 1986. Ciliate conjugation. In: Gall, J. G. (ed.), The Molecular Biology of Ciliated Protozoa. Academic Press, San Diego, Ca. p. 45-84.
16. Peck, R. K. 1977. The cortical ultrastructure of the somatic cortex of Pseudomicrothorax cubius: structure and function of the epiplasm in ciliated protozoa. J. Cell Sci., 25:367-385.
17. Roberts, C. T., Jr. & Orias, E. 1973. Cytoplasmic inheritance of chloramphenicol resistance in Tetrahymena. Genetics, 73:259-272.
18. Shang, Y., Song, X., Bowen, J., Corstanje, R., Gao, Y., Gaertig, J. & Gorovsky, M. 2002. A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proc. Natl. Acad. Sci. USA, 99:3734-3739.
19. Williams, N. E. & Honts, J. E. 1987. The assembly and positioning of cytoskeletal elements in Tetrahymena. Development, 100:23-30.
20. Williams, N. E., Honts, J. E. & Kaczanowska, J. 1990. The formation of basal body domains in the membrane skeleton of Tetrahymena. Development, 109:935-942.
21. Williams, N. E., Vaudaux, P. & Skriver, L. 1979. Cytoskeletal proteins of the cell surface in Tetrahymena. I. Identification and localization of major proteins. Exptl. Cell Res., 123:311-320.
22. Williams, N. E., Honts, J. E., Dress, V. M., Nelsen, E. M. & Frankel, J. 1995. Monoclonal antibodies reveal complex structure in the membrane skeleton of Tetrahymena. J. Eukaryot. Microbiol., 42:422-427.
23. Wolfe, J. 1982. The conjugation junction in Tetrahymena: its structure and development. J. Morphol., 172:159-178.
24. Wuitschick, J. D. & Karrer, K. M. 1999. Analysis of genomic G + C content, codon usage, initiator codon context and translation termination sites in Tetrahymena thermophila. J. Eukaryot. Microbiol., 46: 9-17.