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Volumn 14, Issue 10, 2004, Pages 2563-2566

A novel 11-residual peptaibol-derived carrier peptide for in vitro oligodeoxynucleotide delivery into cell

Author keywords

Antisense; Carrier peptide; Oligodeoxynucleotide delivery; Peptaibol

Indexed keywords

ACETYL ALPHA AMINOISOBUTYRIC ACID ASPARAGINYLISOLEUCYLISOLEUCYL ALPHA AMINOISOBUTYRIC ACID PROLYLLEUCYLLEUCYL ALPHA AMINOBUTYRIC ACID PROLYLISOLEUCYLLYSYLLYSYLLYSYLLYSYLLYSYLLYSYLLYSYLLYSYLLYSYLLYSINE HYDROXIDE; AMINO ACID; ANTISENSE OLIGODEOXYNUCLEOTIDE; CARRIER PROTEIN; DRUG CARRIER; LYSINE; PEPTAIBOL; UNCLASSIFIED DRUG;

EID: 1942534518     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.02.074     Document Type: Article
Times cited : (7)

References (14)
  • 11
    • 1942524239 scopus 로고    scopus 로고
    • note
    • ′-diethyl-9-methyl- thiacarbocyanine bromide; 50 μg/mL in 50% formamide solution) for 12 h, and destained in water for 3 h.
  • 12
    • 1942492254 scopus 로고    scopus 로고
    • note
    • 2. All studies were performed using asynchronous log-phase cultures. Exponentially growing cells were dissociated with trypsin-ethylenediaminetetraacetic acid (EDTA), plated at 50% confluence on glass microscopes slides (Matsunami Glass Ind., Ltd.) and cultured overnight. The culture medium was discarded and the cells were washed three times with DMEM. The cell monolayers were incubated at 37°C with the complex between the carrier peptide and the ODN for 2 h. Subsequently, the cells were rinsed three times with DMEM at room temperature and fixed with paraformaldehyde [3% (w/v) in phosphate-buffered saline (PBS)] for 10 min at room temperature. For experiments at 4°C, the protocol was the same except that all incubations were performed at 4°C until the end of the fixation procedure. Fixed cell monolayers were then washed three times with PBS. The fluorescence distribution was analyzed on a Carl Zeiss LSM510 confocal microscope equipped with a Kr/Ar laser. We also examined with fixed and unfixed cells and the identical intracellular localization of ODN was observed.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.