Improved specimen preparation for cryo-electron microscopy using a symmetric carbon sandwich technique

Journal of Structural Biology

Volumn 146, Issue 3, 2004, Pages 325-333

  Gyobu, Nobuhiko ^a   Tani, Kazutoshi ^a   Hiroaki, Yoko ^a,b   Kamegawa, Akiko ^a,c   Mitsuoka, Kaoru ^a   Fujiyoshi, Yoshinori ^a,c  

^a KYOTO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Aquaporin 4; Cryo electron microscopy; Electron crystallography; Specimen charging; Specimen preparation; Structural analysis; Two dimensional crystal

Indexed keywords

AQUAPORIN 4; CARBON;

ARTICLE; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; FILM COATING; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; RAT; STRUCTURE ANALYSIS; TECHNIQUE; TISSUE SECTION;

AQUAPORIN 4; AQUAPORINS; CARBON; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; SPECIMEN HANDLING;

EID: 1942521362     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsb.2004.01.012     Document Type: Article

References (19)

1. Booy F.P., Pawley J.B. Cryo-crinkling: what happens to carbon films on copper grids at low temperature. Ultramicroscopy. 48:1993;273-280.
2. Brink J., Sherman M.B., Berriman J., Chiu W. Evaluation of charging on macromolecules in electron cryomicroscopy. Ultramicroscopy. 72:1998;41-52.
3. Butt H.J., Wang D.N., Hansma P.K., Kühlbrandt W. Effect of surface roughness of carbon support films on high-resolution electron diffraction of two-dimensional protein crystals. Ultramicroscopy. 36:1991;307-318.
4. Crowther R.A., Henderson R., Smith J.M. MRC image processing programs. J. Struct. Biol. 116:1996;86-92.
5. Doi T., Hiroaki Y., Arimoto I., Fujiyoshi Y., Okamoto T., Satoh M., Furuichi Y. Characterization of human endotheline B receptor and mutant receptors expressed in insect cells. Eur. J. Biochem. 248:1997;139-148.
6. Downing K.H. Spot-scan imaging in transmission electron microscopy. Science. 251:1991;53-59.
7. Fujiyoshi Y. The structural study of membrane proteins by electron crystallography. Adv. Biophys. 35:1998;25-80.
8. Fujiyoshi Y., Mizusaki T., Morikawa K., Yamagishi H., Aoki Y., Kihara H., Harada Y. Development of a superfluid helium stage for high-resolution electron microscopy. Ultramicroscopy. 38:1991;241-251.
9. Glaeser R.M. Specimen flatness of thin crystalline arrays: influence of the substrate. Ultramicroscopy. 46:1992;33-44.
10. Henderson R. Image contrast in high-resolution electron microscopy of biological macromolecules: TMV in ice. Ultramicroscopy. 46:1992;1-18.
11. Henderson R., Baldwin J.M., Downing K.H., Lepault J., Zemlin F. Structure of purple membrane from Halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5. Å resolution Ultramicroscopy. 19:1986;147-178.
12. Henderson R., Baldwin J.M., Ceska T.A., Zemlin F., Beckmann E., Downing K.H. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213:1990;899-929.
13. Koning R.I., Oostergetel G.T., Brisson A. Preparation of flat carbon support films. Ultramicroscopy. 94:2003;183-191.
14. Kühlbrandt W., Wang D.N., Fujiyoshi Y. Atomic model of plant light-harvesting complex by electron crystallography. Nature. 389:1994;614-621.
15. Mitsuoka K., Hirai T., Murata K., Miyazawa A., Kidera A., Kimura Y., Fujiyoshi Y. The structure of bacteriorhodopsin at 3.0. Å resolution based on electron crystallography: implication of the charge distribution J. Mol. Biol. 286:1999;861-882.
16. Murata K., Mitsuoka K., Hirai T., Walz T., Agre P., Heymann J.B., Engel A., Fujiyoshi Y. Structural determinants of water permeation through aquaporin-1. Nature. 407:2000;599-605.
17. Nogales E., Wolf S.G., Downing K.H. Structure of. αβ tubulin dimer by electron crystallography Nature. 391:1998;199-203.
18. Schmutz M., Brisson A. Analysis of carbon film planarity by reflection light microscopy. Ultramicroscopy. 63:1996;263-272.
19. Walz T., Grigorieff N. Electron crystallography of two-dimensional crystals of membrane proteins. J. Struct. Biol. 121:1998;142-161.