Indiscriminate glycosylation of procarboxypeptidase Y expressed in Pichia pastoris

Carbohydrate Research

Volumn 339, Issue 6, 2004, Pages 1041-1045

  Maeda, Haruko ^a   Chatani, Eri ^a   Koyama, Takahiro ^a   Sugiura, Masatoshi ^a   Izumi, Hirohisa ^a   Hayashi, Rikimaru ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

BTpNA, N benzoil L tyrosine p nitroanilide; Carboxypeptidase Y; CD, circular dichroism; Con A, concanavalin A; EDTA, ethylenediaminetetraacetic acid; N Glycosylation site; O Glycosylation site; PEG, polyethylene glycol; Pichia pastoris; Procarboxypeptidase Y

Indexed keywords

ENZYMES; LIGHT SCATTERING; PHENOLS; POLYPEPTIDES; SULFURIC ACID;

GLYCOSYLATION; POINT MUTATION METHODS; RECOMBINANT PROCESSES;

CARBOHYDRATES;

ALANINE; PHENOL; PROCARBOXYPEPTIDASE Y; PROTEIN PRECURSOR; RECOMBINANT ENZYME; SERINE; SULFURIC ACID; THREONINE; UNCLASSIFIED DRUG;

ABSORPTION; AMINO ACID SUBSTITUTION; ANALYTICAL EQUIPMENT; ARTICLE; CARBOHYDRATE ANALYSIS; ENZYME GLYCOSYLATION; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; LIGHT SCATTERING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PICHIA PASTORIS; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ALANINE; CARBOHYDRATES; CARBOXYPEPTIDASE C; CIRCULAR DICHROISM; CONCANAVALIN A; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUCOSE; GLYCOSYLATION; LIGHT; PHENOL; PICHIA; PLASMIDS; POINT MUTATION; PROTEIN PRECURSORS; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

PICHIA; PICHIA PASTORIS;

EID: 1942485879     PISSN: 00086215     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carres.2004.02.003     Document Type: Article

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