Expression of Treponema denticola Oligopeptidase B in Escherichia coli

Current Microbiology

Volumn 48, Issue 5, 2004, Pages 379-382

  Lee, Si Young ^a,b   Fenno, J Christopher ^b  

^a Gangneung Wonju National University   (South Korea)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGOPEPTIDASE B; PEPTIDASE; TRYPTASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PERIODONTAL DISEASE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TREPONEMA DENTICOLA;

BACTERIAL PROTEINS; BENZOYLARGININE NITROANILIDE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; TRANSFORMATION, BACTERIAL; TREPONEMA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SPIROCHAETALES; TREPONEMA; TREPONEMA DENTICOLA;

EID: 1942453726     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-003-4168-4     Document Type: Article

References (12)

1. Egli C, Leung WK, Müller KH, Hancock RE, McBride BC (1993) Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola. Infect Immun 61:1694-1699
2. Fenno JC, McBride BC (1998) Virulence factors of oral treponemes. Anaerobe 4:1-17
3. Fenno JC, Lee SY, Bayer CH, Ning Y (2001) The opdB locus encodes the trypsin-like peptidase activity of Treponema denticola. Infect Immun 69:6193-6200
4. Loesche WJ, Syed SA, Stoll J (1987) Trypsin-like activity in subgingival plaque. A diagnostic marker for spirochetes and periodontal disease? J Periodontol 58:266-273
5. Loesche WJ, Bretz WA, Lopatin D, Stoll J, Rau CF, Hillenburg KL, Killoy WJ, Drisko CL, Williams R, Weber HP (1990a) Multicenter clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease. J Periodontol 61:189-196
6. Loesche WJ, Giordano J, Hujoel PP (1990b) The utility of the BANA test for monitoring anaerobic infections due to spirochetes (Treponema denticola) in periodontal disease. J Dent Res 69: 1696-1702
7. Mäkinen KK, Mäkinen PL (1996) The peptidolytic capacity of the spirochete system. Med Microbiol Immunol 185:1-10
8. Mäkinen KK, Chen CY, Mäkinen PL, Ohta K, Loesche WJ (1990) The benzoylarginine peptidase from Treponema denticola (strain ASLM), a human oral spirochaete: evidence for active-site carboxyl groups. Mol Microbiol 4:1413-1417
9. Mäkinen KK, Mäkinen PL, Loesche WJ, Syed SA (1995) Purification and general properties of an oligopeptidase from Treponema denticola ATCC 35405-a human oral spirochete. Arch Biochem Biophys 316:689-698
10. Ohta K, Mäkinen KK, Loesche WJ (1986) Purification and characterization of an enzyme produced by Treponema denticola capable of hydrolyzing synthetic trypsin substrates. Infect Immun 53:213-220
11. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr (1998) Microbial complexes in subgingival plaque. J Clin Periodontol 25:134-144
12. Uitto V-J, Grenier D, Chan EC, McBride BC (1988) Isolation of a chymotrypsinlike enzyme from Treponema denticola. Infect Immun 56:2717-2722