Cellophane based mini-prep method for DNA extraction from the filamentous fungus Trichoderma reesei

BMC Microbiology

Volumn 2, Issue , 2002, Pages 1-4

  Cassago, Alexandre ^a   Panepucci, Rodrigo Alexandre ^a   Baião, Ana Maria Tortella ^a   Henrique Silva, Flavio ^a  

^a FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CELLOPHANE; FUNGAL DNA; GLASS; MICROSPHERE; NITROGEN;

ARTICLE; CELL DISRUPTION; CELL WALL; CONTROLLED STUDY; DNA CONTENT; DNA DETERMINATION; DNA EXTRACTION; FUNGAL GENE; HPH GENE; MYCELIAL GROWTH; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TECHNIQUE; TRICHODERMA REESEI; CRYOPRESERVATION; CULTURE MEDIUM; GENETICS; GROWTH, DEVELOPMENT AND AGING; INSTRUMENTATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MYCOLOGY; TRICHODERMA;

FUNGI; HYPOCREA JECORINA; TRICHODERMA;

CELL WALL; CELLOPHANE; CRYOPRESERVATION; CULTURE MEDIA; DNA, FUNGAL; GLASS; MICROSPHERES; MYCOLOGY; NITROGEN; TRICHODERMA;

EID: 19044388563     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-2-14     Document Type: Article

References (14)

1. Specht CA, DiRusso CC, Novotny CP, Ullrich RC: A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem 1982, 119:158-163
2. de Graaff L, van den BH, Visser J: Isolation and transformation of the pyruvate kinase gene of Aspergillus nidulans. Curr Genet 1988, 13:315-321
3. Bainbridge BW, Spreadbury CL, Scalise FG, Cohen J: Improved methods for the preparation of high molecular weight DNA from large and small scale cultures of filamentous fungi. FEMS Microbiol Lett 1990, 54:113-117
4. Challen MP, Moore AJ, Martinez-Carrera D: Facile extraction and purification of filamentous fungal DNA. Biotechniques 1995, 18:975-978
5. Raeder U, Broda P: Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1985, 17-20
6. Nijs M, Nabben L, Wernars K: Isolation of Fusarium DNA for molecular analysis with and without mechanical cell disruption. Journal of Microbiological Methods 1996, 27:13-17
7. Liu D, Coloe S, Baird R, Pedersen J: Rapid Mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology 2000, 38:471
8. Cenis JL: Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 1992, 20:2380
9. Müller FMC, Werner KE, Kasai M, Francesconi A, Chanock SJ, Walsh TJ: Rapid Extraction of Genomic DNA from Medically Important Yeasts and Filamentous Fungi by High-Speed Cell Disruption. Journal of Clinical Microbiology 1998, 36:1625-1629
10. Lecellier G, Silar P: Rapid methods for nucleic acids extraction from Petri dish-grown mycelia. Current Genetics 1994, 25:122-123
11. Pentilla M, Nevalainen H, Ratto M, Salminen E, Knowles J: A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 1987, 61:155-164
12. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988, 239:487-491
13. Gritz L, Davies J: Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisae. Gene 1983, 25:179-188
14. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A laboratory manual Cold Spring Harbor Laboratory Press 1989