Making cell-permeable recombinant telomerase (trans-telomerase) through fusion of its catalytic subunit (hTERT) with protein transduction domains (PTD): A possible strategy to overcome replicative senescence during ex vivo culture of primary explanted cells [10]

Medical Hypotheses

Volumn 65, Issue 1, 2005, Pages 199-200

  Boon, Chin Heng ^a   Cao, Tong ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; RECOMBINANT DNA; RECOMBINANT PROTEIN; TELOMERASE;

CATALYSIS; CELL COUNT; CELL DIVISION; CELL LINEAGE; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; DNA MODIFICATION; EX VIVO STUDY; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; IN VITRO STUDY; INTERNALIZATION; LETTER; LIFESPAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SENESCENCE; SIGNAL TRANSDUCTION; STEM CELL TRANSPLANTATION; X IRRADIATION;

CATALYTIC DOMAIN; CELL AGING; CELLS, CULTURED; HUMANS; MODELS, BIOLOGICAL; PERMEABILITY; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SIGNAL TRANSDUCTION; TELOMERASE; TELOMERE;

EID: 18944390905     PISSN: 03069877     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mehy.2005.01.022     Document Type: Letter

References (5)

1. Y. Yang, Y. Chen, C. Zhang, H. Huang, and S.M. Weissman Nucleolar localization of hTERT protein is associated with telomerase function Exp Cell Res 277 2 2002 201 209
2. S. Franco, K.L. MacKenzie, S. Dias, S. Alvarez, S. Rafii, and M.A. Moore Clonal variation in phenotype and life span of human embryonic fibroblasts (MRC-5) transduced with the catalytic component of telomerase (hTERT) Exp Cell Res 268 1 2001 14 25
3. R.D. Ramirez, S. Sheridan, L. Girard, M. Sato, Y. Kim, and J. Pollack Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins Cancer Res 64 24 2004 9027 9034
4. J. Spink, and D. Geddes Gene therapy progress and prospects: bringing gene therapy into medical practice: the evolution of international ethics and the regulatory environment Gene Ther 11 22 2004 1611 1616
5. A. Joliot, and A. Prochiantz Transduction peptides: from technology to physiology Nat Cell Biol 6 3 2004 189 196