Isolation of chicken primordial germ cells by centrifugation in ficoll gradient

Zivocisna Vyroba

Volumn 42, Issue 9, 1997, Pages 399-401

  Trefil, P ^a   Kotrbova A ^a   Juzova S ^a  

^a INSTITUTE OF ANIMAL SCIENCE   (Czech Republic)

Author keywords

Centrifugation; Chicken; Primordial germ cells

Indexed keywords

EID: 18944382155     PISSN: 00444847     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. ALLIOLI, N. - THOMAS, J. L. - CHEBLOUNE, Y. - NIGON, V. M. - VERDIER, G. - LEGRAS, C.: Use of retroviral vectors to introduce and express the β-galactosidase marker gene in cultured chicken primordial germ cells. Dev. Biol., 165, 1994: 30-37.
2. CHANG, I. K. - TAJIMA, A. - YASUDA, Y. - CHIKAMUNE, T. - OHNO, T.: Simple method for isolation of primordial germ cells from chick embryos. Cell Biol. Int. Rep., 16, 1992: 853-857.
3. EYAL-GILADI, H. - KOCHAV, S.: From cleavage to primitive streak formation: A complementary normal table and a new look at the first stages of the development of the chick. Dev. Biol., 49, 1976: 321-337.
4. EYAL-GILADI, H. - GINSBURG, M. - FARBAROV, A.: Avian primordial germ cells are epiblastic origin. J. Embryol. Exp. Morphol., 65, 1981: 139-147.
5. HAMBURGER, V. - HAMILTON, H. L.: A series of normal stages in the development of the chick embryo. J. Morphol., 88, 1951: 49-92.
6. MEYER, D. B.: Application of the periodic acid-Schiff technique to whole chick embryos. Stain Technol., 35, 1960: 83-89.
7. WALDEYER, W. - Engelmann, W. (eds.): Eierstock und Ei. Ein Beitrag zur Anatomie und Entwicklungs - Geschichte der sexual Organe. G.D.R. Leipzig, 1870.
8. WENTWORTH, B. C. - TSAI, H. - HALLETT, J. H. -GONZALES, D. S. - RAJCIC-SPASOJEVIC, G.: Manipulation of avian primordial germ cells and gonadal differentiation. Poult. Sci., 68, 1989: 999-1010.