Efficient Preparation of Maritime Pine (Pinus pinaster) Protoplasts Suitable for Transgene Expression Analysis

Plant Molecular Biology Reporter

Volumn 19, Issue 4, 2001, Pages 361-366

  Gómez Maldonado, Josefa ^a   Crespillo, Remedios ^a   Ávila, Concepción ^a   Cánovas, Francisco M ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

Author keywords

Electroporation; Pine seedlings; Pinus pinaster; Promoter analysis; Shoot protoplasts

Indexed keywords

EID: 18844479645     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02772834     Document Type: Article

References (14)

1. Avila C, Muñoz-Chápuli R, Plomion C, Frigerio J-M, and Cánovas FM (2000) Two genes encoding distinct cytosolic glutamine synthetases are closely linked in the pine genome. FEBS Lett 477: 237-243.
2. Avila C, Suárez M-F, Gómez-Maldonado J, and Cánovas FM (2001) Spatial and temporal expression of two cytosolic glutamine synthetase genes in Scots pine: functional implications on nitrogen metabolism during early stages of conifer development. Plant J 25: 93-102.
3. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
4. Cánovas FM, Cantón FR, Gallardo F, García-Gutiérrez A, and de Vicente A (1991) Accumulation of glutamine synthetase during early development of maritime pine (Pinus pinaster) seedlings. Planta 185: 372-378.
5. David H, David A, and Mateille T (1982) Evaluation of parameters affecting the yield, viability and division of Pinus pinater protoplasts. Physiol Plant 56: 108-113.
6. Duhoux E (1980) Protoplast isolation of gymnosperm pollen. Z Pflanzenphysiol 99: 207-214.
7. Faye E and David A (1983) Isolation and culture of gymnosperm root protoplasts (Pinus pinaster). Physiol Plant 59: 359-362.
8. Gupta PK and Durzan DJ (1986) Isolation and cell regeneration of protoplasts from sugar pine (Pinus lambertiana). Plant Cell Rep 5: 346-348.
9. Hakman IC, von Harnold S, and Fellner-Feldegg H (1986) Isolation and DNA analysis of protoplasts from developing female gametophytes of Picea abies (Norway spruce). Plant Sci 38: 53-59.
10. Jefferson RA, Kavanagh TA, and Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile fusion marker in higher plants. EMBO J 7: 1265-1271.
11. Mitsuhara I, Ugaki M, Hirochika H, Oshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, Ueno K, Mochizuki A, Tanimoto H, Tsugawa H, Otsuki Y, and Ohashi Y (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol 37: 49-59.
12. Patel KR, Shekhawat NS, Berlyn GP, and Thorpe TA (1984) Isolation and culture of protoplasts from cotyledons of Pinus coulteri. Plant Cell Tissue Organ Cult 3: 85-90.
13. Rotman BR and Papermaster RD (1966) Membrane properties of living cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc Natl Acad Sci USA 55: 134-141.
14. Shen WJ, Williamson MS, and Forde BG (1992) Functional analysis of the promoter region of a nodule-enhanced glutamine synthetase gene from Phaseolus vulgaris L. Plant Mol Biol 19: 837-846.