Real-time fluorescence polymerase chain reaction (PCR) identification of Streptococcus pneumoniae from pleural fluid and tissue

Scandinavian Journal of Infectious Diseases

Volumn 37, Issue 5, 2005, Pages 391-392

  Poulter, Melinda D ^a   Deville, Jaime G ^a,c   Cherry, James D ^a   Shapiro, Alan M ^a   McAvin, James C ^b  

^a UCLA MEDICAL CENTER   (United States)

^b Air Force Institute for Environmental and Occupational Health Risk Analysis   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; CEFOTAXIME; NAFCILLIN; VANCOMYCIN;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; CASE REPORT; CLINICAL FEATURE; DEBRIDEMENT; ENDOSCOPIC SURGERY; FEMALE; HUMAN; HUMAN TISSUE; LABORATORY TEST; LOBAR PNEUMONIA; NONHUMAN; NUCLEIC ACID ANALYSIS; PLEURA EFFUSION; PLEURA EMPYEMA; PLEURA FLUID; PRESCHOOL CHILD; REAL TIME POLYMERASE CHAIN REACTION; SINGLE DRUG DOSE; STREPTOCOCCUS PNEUMONIAE; THORACOCENTESIS; THORACOSCOPY; THORAX DRAINAGE;

BACTERIAL TYPING TECHNIQUES; CHILD, PRESCHOOL; DNA, BACTERIAL; EMPYEMA, PLEURAL; FEMALE; FLUORESCENCE; HUMANS; PLEURA; PLEURAL EFFUSION; PNEUMONIA, PNEUMOCOCCAL; POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE;

EID: 18844437867     PISSN: 00365548     EISSN: None     Source Type: Journal    
DOI: 10.1080/00365540510031683     Document Type: Article

References (5)

1. McAvin JC, Reilly PA, Roudabush RM, Barnes WJ, Salmen A, Jackson GW, et al. Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR. J Clin Microbiol 2001;39:3446-51.
2. Higuchi R. Simple and rapid preparation of samples for PCR. In: Erlich HA, editor. PCR technology: principles and applications for DNA amplification. New York, Ny: Stockton Press; 1989. p. 31-8.
3. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques 1997;22:130-8.
4. Daum LT, Barnes WJ, McAvin JC, Neidert MS, Copper LA, Huff WB, et al. Real-time PCR detection of Salmonella in suspected foods from a gastroenteritis outbreak in Kerr County, Texas. J Clin Microbiol 2002;40:1-3.
5. Cooper LA, Atchley DH, Hadfield TL, Lohman KL. Detection of Bacillus anthracis in veterinary clinical samples using field deployable real-time fluorogenic PCR. [Abstract 62-110]. Presented at the International Conference on Emerging Infectious Diseases: ICEID, Atlanta, GA, March 24-27, 2002.