In vivo imaging of axonal degeneration and regeneration in the injured spinal cord

Nature Medicine

Volumn 11, Issue 5, 2005, Pages 572-577

  Kerschensteiner, Martin ^a,b   Schwab, Martin E ^b   Lichtman, Jeff W ^a   Misgeld, Thomas ^a  

^a HARVARD UNIVERSITY   (United States)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IMAGING; MOUSE; NERVE DEGENERATION; NERVE FIBER DEGENERATION; NERVE FIBER GROWTH; NERVE FIBER REGENERATION; NEUROPATHOLOGY; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD INJURY; TRANSGENIC MOUSE;

ANIMALS; AXONS; CALPAIN; GANGLIA, SPINAL; GLYCOPROTEINS; GREEN FLUORESCENT PROTEINS; IMMUNOHISTOCHEMISTRY; MICE; MICE, TRANSGENIC; MICROSCOPY, FLUORESCENCE; NERVE DEGENERATION; NERVE REGENERATION; SPINAL CORD; SPINAL CORD INJURIES; TIME FACTORS;

ALBURNUS ALBURNUS; ANIMALIA; MUS MUSCULUS; WALLERIA;

EID: 18844436224     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/nm1229     Document Type: Article

References (23)

1. Ramón y Cajal, S. Degeneration and regeneration of white matter in Cajal's Degeration and Regeneration of the Nervous System (eds DeFelipe, J. & Jones, E.G.) ch.4 (Oxford Univ. Press, New York, 1991).
2. Steward, O., Zheng, B. & Tessier-Lavigne, M. False resurrections: distinguishing regenerated from spared axons in the injured central nervous system. J. Comp. Neurol. 459, 1-8 (2003).
3. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51 (2000).
4. Neumann, S. & Woolf, C.J. Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron 23, 83-91 (1999).
5. Qiu, J. et al. Spinal axon regeneration induced by elevation of cyclic AMP. Neuron 34, 895-903 (2002).
6. Walsh, M.K. & Lichtman, J.W. In vivo time-lapse imaging of synaptic takeover associated with naturally occurring synapse elimination. Neuron 37, 67-73 (2003).
7. Tom, V.J., Steinmetz, M.P., Miller, J.H., Doller C.M. & Silver J. Studies on the development and behavior of the dystrophic growth cone, the hallmark of regeneration failure, in an in vitro model of the glial scar and after spinal cord injury. J. Neurosci. 24, 6531-6539 (2004)
8. Firkins, S.S., Bates, C.A., & Stelzner, D.J. Corticospinal tract plasticity and astroglial reactivity after cervical spinal injury in the postnatal rat. Exp. Neurol. 120, 1-15 (1993).
9. Demjen, D. et al. Neutralization of CD95 ligand promotes regeneration and functional recovery after spinal cord injury. Nat. Med. 10, 389-395 (2004).
10. Houle, J.D. & Jin, Y. Chronically injured supraspinal neurons exhibit only modest axonal dieback in response to a cervical hemisection lesion. Exp. Neurol. 169, 208-217 (2001).
11. Perry, V.H., Brown, M.C., & Lunn, E.R. Very slow retrograde and Wallerian degeneration in the CNS of C57BL/Ola mice. Eur. J. Neurosci. 3, 102-105 (1991).
12. Mack, T.G. et al. Wallerian degeneration of injured axons and synapses is delayed by a Ube4b/Nmnat chimeric gene. Nat. Neurosci. 4, 1199-1206 (2001).
13. George, E.B., Glass, J.D. & Griffin, J.W. Axotomy-induced axonal degeneration is mediated by calcium influx through ion-specific channels. J. Neurosci. 15, 6445-6452 (1995).
14. Zhai, Q. et al. Involvement of the ubiquitin-proteasome system in the early stages of Wallerian degeneration. Neuron 39, 217-225 (2003).
15. Saido, T.C. et al. Spatial resolution of fodrin proteolysis in postischemic brain. J. Biol. Chem. 268, 25239-25243 (1993).
16. Hill, C.E., Beattie, M.S. & Bresnahan, J.C. Degeneration and sprouting of identified descending supraspinal axons after contusive spinal cord injury in the rat. Exp. Neurol. 171, 153-169 (2001).
17. Li, Y. & Raisman, G. Sprouts from cut corticospinal axons persist in the presence of astrocytic scarring in long-term lesions of the adult rat spinal cord. Exp. Neurol. 134, 102-111 (1995).
18. Pan, Y.A., Misgeld, T., Lichtman J.W. & Sanes, J.R. Effects of neurotoxic and neuroprotective agents on peripheral nerve regeneration assayed by time-lapse imaging in vivo. J. Neurosci. 23, 11479-11488 (2003)
19. Raff, M.C., Whitmore, A.V. & Finn, J.T. Axonal self-destruction and neurodegeneration. Science 296, 868-871 (2002).
20. Bareyre, F.M. et al. The injured spinal cord spontaneously forms a new intraspinal circuit in adult rats. Nat. Neurosci. 7, 269-277 (2004).
21. Nguyen, Q.T., Sanes, J.R. & Lichtman, J.W. Pre-existing pathways promote precise projection patterns. Nat. Neurosci. 5, 861-867 (2002).
22. Beirowski, B. et al. Quantitative and qualitative analysis of Wallerian degeneration using restricted axonal labelling in YFP-H mice. J. Neurosci. Meth. 134, 23-35 (2004).
23. Gillingwater, T.H. et al. Age-dependent synapse withdrawal at axotomised neuromuscular junctions in Wld(s) mutant and Ube4b/Nmnat transgenic mice. J. Physiol. 543, 739-755 (2002).