Optimization of an enzyme-linked immunosorbent assay for ecdysteroids

Journal of Insect Biotechnology and Sericology

Volumn 74, Issue 1, 2005, Pages 1-4

  Shiotsuki, Takahiro ^a   Hua, Yue Jin ^a,c   Tsugane, Taneaki ^a,d   Gee, Shirley ^b   Hammock, Bruce D ^b  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c ZHEJIANG UNIVERSITY   (China)

^d Chiba Prefectural Agriculture Research Center   (Japan)

Author keywords

Agrius convolvuli; Bombyx mori; Ecdysteroid titer; Enzyme linked immunosorbent assay (ELISA)

Indexed keywords

AGRIUS CONVOLVULI; BOMBYX MORI; COMPETITION ASSAYS; ECDYSTEROID TITER; ENZYME LINKED IMMUNOSORBENT ASSAY; HORSERADISH PEROXIDASE; INDIRECT METHODS; RABBIT ANTISERUM; MANDUCA SEXTA;

STEROID HORMONES;

OPTIMIZATION;

AGRIUS CONVOLVULI; ARMORACIA RUSTICANA; BOMBYX MORI; CONVOLVULUS; MANDUCA SEXTA; ORYCTOLAGUS CUNICULUS;

BIOTECHNOLOGY; ENZYME; OPTIMIZATION;

EID: 18844432174     PISSN: 13468073     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Bollenbacher, W.E., Smith, S.L., Goodman, W., and Gilbert, L.I. (1981) Ecdysteroid titer during larval-pupal-adult development of the tobacco hornworm, Manduca sexta. Gen. Comp. Endocrinol. 44, 302-306.
2. Harlow, E., and Lane, D. (1988) "Antibodies: A laboratory handbook", Cold Spring Harbor Laboratory, New York, pp. 553-612.
3. Kiguchi, K., and Shimoda, M. (1994) The sweet potato hornworm, Agrius convolvuli, as a new experimental insect: continuous rearing using artificial diets. Zool. Sci. 11, 143-147.
4. Kingan, T.G. (1989) A competitive enzyme-linked immunosorbent assay: Applications in the assay of peptides, steroids, and cyclic nucleotides. Anal. Biochem. 183, 283-289.
5. Pascual, N., Belles, X., Delbecque, J.P., Hua Y-J., and Koolman, J. (1995) Quantification of Ecdysteroids by immunoassay: Comparison of enzyme immunoassay and radioimmunoassay. Z. Naturforsch. [C] 50 , 862-867.
6. Soumoff, C., Horn, D.H.S., and O'Connor, J.D. (1981) Production of a new antiserum to arthropod molting hormone and comparison with two other antisera. J. Steroid Biochem. 14, 429-435.
7. Takeda, S., Kiuchi, M., and Ueda, S. (1986) Preparation of anti-20-hydroxyecdysone serum and its application for radioimmunoassay of ecdysteroids in silkworm hemolymph. (in Japanese with English summary) Bull. Sericultural Exp. Sta. 30, 361-374.
8. Von Gliscynski, U., Delbecque, J.P., Bocking, D., Sedlmeier, D., Dircksen, H., and Lafont, R. (1995) Three new antisera with high sensitivity to ecdysone, 3-dehydroxy- ecdysone and other A-ring derivatives: Production and characterization. Eur. J. Entomol. 92, 75-79.