Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: An example of application for protein chemistry

Journal of Biochemical and Biophysical Methods

Volumn 63, Issue 1, 2005, Pages 33-42

  Hasegawa, Go ^a   Kikuchi, Masashi ^a   Kobayashi, Yuichi ^a   Saito, Yuji ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Antibody; Dansyl group; Fluorescent labeling; Sulfhydryl reagent

Indexed keywords

5,5' DITHIOBIS(NITROBENZOIC ACID); ANTIBODY; CYSTEINE; DANSYL CHLORIDE; DANSYLCADAVERINE; DICYCLOHEXYLCARBODIIMIDE; FLUORESCENT DYE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; IODOACETIC ACID; IODOACETYL DANSYLCADAVERINE; LYSYL ENDOPEPTIDASE; NITROBENZOIC ACID DERIVATIVE; PEPTIDE; PROTEIN; PROTEINASE; REAGENT; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ALKYLATION; AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ARTICLE; CHEMICAL ENGINEERING; CHEMICAL REACTION; CHEMICAL STRUCTURE; CHEMISTRY; CHICKEN; DATA ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR INTERACTION; MUSCLE TISSUE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN INTERACTION; PROTEIN METABOLISM; STRUCTURE ANALYSIS; SYNTHESIS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CADAVERINE; CHICKENS; CYSTEINE; DANSYL COMPOUNDS; DITHIONITROBENZOIC ACID; GLUTATHIONE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); MOLECULAR SEQUENCE DATA; MUSCLES; PEPTIDE FRAGMENTS; PROTEINS; SENSITIVITY AND SPECIFICITY; SULFHYDRYL COMPOUNDS; SULFHYDRYL REAGENTS;

GALLUS GALLUS;

EID: 18844390290     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2005.01.001     Document Type: Article

References (18)

1. D.J. Pouchnik, L.E. Laverman, F. Janiak-Spens, D.M. Jameson, G.D. Reinhart, and C.R. Cremo Synthesis and spectral characterization of sulfhydryl-reactive fluorescent probes Anal Biochem 235 1996 26 35
2. I. Pavo, E. Kojro, and F. Fahrenholz Synthesis and binding characteristics of two sulfhydryl-reactive probes for vasopressin receptors FEBS Lett 316 1993 59 62
3. R.J. Krueger, C. Lin, and J.A. Frank Synthesis and properties of sulfhydryl-group-specific reagents containing 125I Anal Biochem 198 1991 165 173
4. R.B. Moreland, P.K. Smith, E.K. Fujimoto, and M.E. Dockter Synthesis and characterization of N-(4-azidophenylthio)phthalimide: a cleavable, photoactivable crosslinking reagent that reacts with sulfhydryl groups Anal Biochem 121 1982 321 326
5. N.S. Kosower, E.M. Kosower, G.L. Newton, and H.M. Ranney Bimane fluorescent labels: labeling of normal human red cells under physiological conditions Proc Natl Acad Sci U S A 76 1979 3382 3386
6. E.N. Hudson, and G. Weber Synthesis and characterization of two fluorescent sulfhydryl reagents Biochemistry 12 1973 4154 4161
7. Y. Kanaoka, M. Machida, Y. Ban, and S. Takamitsu Fluorescence and structure of proteins as measured by incorporation of fluorophore: II. Synthesis of maleimide derivatives as fluorescence-labeled protein-sulfhydryl reagents Chem Pharm Bull (Tokyo) 15 1967 1738 1743
8. N. Seiler Use of the dansyl reaction in biochemical analysis Methods Biochem Anal 18 1970 259 337
9. J. Takagi, Y. Saito, T. Kikuchi, and Y. Inada Modification of transglutaminase assay: use of ammonium sulfate to stop the reaction Anal Biochem 153 1986 295 298
10. V.P. Pigiet, and B.J. Schuster Thioredoxin-catalyzed refolding of disulfide-containing proteins Proc Natl Acad Sci U S A 83 1986 7643 7647
11. G. Hasegawa, M. Suwa, Y. Ichikawa, T. Ohtsuka, S. Kumagai, and M. Kikuchi A novel function of tissue-type transglutaminase: protein disulphide isomerase Biochem J 373 2003 793 803
12. P.W. Riddles, R.L. Blakeley, and B. Zerner Reassessment of Ellman's reagent Methods Enzymol 91 1983 49 60
13. C. Gros, and B. Labouesse Study of the dansylation reaction of amino acids, peptides and proteins Eur J Biochem 7 1969 463 470
14. R.F. Chen Dansyl labeled proteins: determination of extinction coefficient and number of bound residues with radioactive dansyl chloride Anal Biochem 25 1968 412 416
15. R.N. Perham, and J.I. Harris Amino acid sequences around the reactive cysteine residues in glyceraldehyde-3-phosphate dehydrogenases J Mol Biol 16 1963 316 320
16. A. Dugaiczyk, J.A. Haron, E.M. Stone, O.E. Dennison, K.N. Rothblum, and R.J. Schwartz Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle Biochemistry 22 1983 1605 1613
17. K. Shimada, and K. Mitamura Derivatization of thiol-containing compounds J Chromatogr, B, Biomed Appl 659 1994 227 241
18. D.J. Marsh, and S. Lowey Fluorescence energy transfer in myosin subfragment-1 Biochemistry 19 1980 774 784