Simple biodosimetry method for use in cases of high-dose radiation exposure that scores the chromosome number of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC)

International Journal of Radiation Biology

Volumn 81, Issue 1, 2005, Pages 33-40

  Gotoh, E ^a,b   Tanno, Y ^c   Takakura, K ^c  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c INTERNATIONAL CHRISTIAN UNIVERSITY   (Japan)

Author keywords

Biodosimeter; Calyculin A; Chromosome number; Drug induced premature chromosome condensation; Giemsa staining; High dose exposure accident

Indexed keywords

CALYCULIN A; PHYTOHEMAGGLUTININ P;

ADULT; ARTICLE; CELL DIVISION; CHROMOSOME NUMBER; CONTROLLED STUDY; DECISION MAKING; DOSIMETRY; FEMALE; GAMMA IRRADIATION; GIEMSA STAIN; HUMAN; HUMAN CELL; IN VITRO STUDY; MALE; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; PREMATURE CHROMOSOME CONDENSATION; PRIORITY JOURNAL; RADIATION ACCIDENT; RADIATION DOSE; RADIATION EXPOSURE; WHOLE BODY RADIATION;

ADULT; AZURE STAINS; CELL CYCLE PROTEINS; CHROMOSOME ABERRATIONS; CHROMOSOMES; COLORING AGENTS; FEMALE; GAMMA RAYS; HUMANS; KARYOTYPING; LYMPHOCYTES; MALE; MIDDLE AGED; MITOGENS; OXAZOLES; PHYTOHEMAGGLUTININS; RADIATION INJURIES; RADIOMETRY;

EID: 18744395249     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/09553000500092319     Document Type: Article

References (31)

1. Anno GH, Young RW, Bloom RM, Mercier JR. 2003. Dose-response relationships for acute ionizing-radiation lethality. Health Physics 84: 565-575.
2. Asakawa Y, Gotoh E. 1997. A method for detecting sister chromatid exchanges using prematurely condensed chromosomes and immunogold-silver staining. Mutagenesis 12: 175-177.
3. Begg AC, Sprong D, Balm A, Martin JM. 2002. Premature chromosome condensation and cell separation studies in biopsies from head and neck tumors for radiosensitivity prediction. Radiotherapy Oncology 62: 335-343.
4. Bezrookove V, Smits R, Moeslein G, Fodde R, Tanke HJ, Raap AK, Darroudi F. 2003. Premature chromosome condensation revisited: A novel chemical approach permits efficient cytogenetic analysis of cancers. Genes Chromosomes and Cancer 38: 177-186.
5. Blakely WF, Prasanna PG, Kolanko CJ, Pyle MD, Mosbrook DM, Loats AS, Rippeon TL, Loats H. 1995. Application of the premature chromosome condensation assay in simulated partial-body radiation exposures: evaluation of the use of an automated metaphase-finder. Stem Cells 13(Suppl. 1): 223-230.
6. Blakely WF, Prasanna PG, Grace MB, Miller AC. 2001. Radiation exposure assessment using cytological and molecular biomarkers. Radiation Protection Dosimetry 97: 17-23.
7. Coco-Martin JM, Begg AC. 1997. Detection of radiation-induced chromosome aberrations using fluorescence in situ hybridization in drug-induced premature chromosome condensations of tumour cell lines with different radiosensitivities. International Journal of Radiation Biology 71: 265-273.
8. Cornforth MN, Bedford JS. 1983. X-ray-induced breakage and rejoining of human interphase chromosomes. Science 222: 1141-1143.
9. Darroudi F, Natarajan AT, Bentvelzen PA, Heidt PJ, Van Rotterdam A, Zoetelief J, Broerse JJ. 1998. Detection of total-and partial-body irradiation in a monkey model: a comparative study of chromosomal aberration, micronucleus and premature chromosome condensation assays. International Journal of Radiation Biology 74: 207-215.
10. Gotoh E, Asakawa Y, Kosaka H. 1995. Inhibition of protein serine/threonine phosphatases directly induces premature chromosome condensation in mammalian somatic cells. Biomedical Research 16: 63-68.
11. Gotoh E, Asakawa Y. 1996. Detection and evaluation of chromosomal aberrations induced by high doses of gamma-irradiation using immunogold-silver painting of prematurely condensed chromosomes. International Journal of Radiation Biology 70: 517-520.
12. Gotoh E, Kawata T, Durante M. 1999. Chromatid break rejoining and exchange aberration formation following gamma-ray exposure: analysis in G2 human fibroblasts by chemically induced premature chromosome condensation. International Journal of Radiation Biology 75: 1129-1135.
13. Hayata I, Kanda R, Minamihisamatsu M, Furukawa M, Sasaki MS. 2001. Cytogenetical dose estimation for 3 severely exposed patients in the JCO criticality accident in Tokai-mura. Journal of Radiation Research (Tokyo) 42(Suppl.): S149-155.
14. Hittelman WN, Rao PN. 1974. Premature chromosome condensation. I. Visualization of x-ray-induced chromosome damage in interphase cells. Mutation Research 23: 251-258.
15. Iliakis GE, Pantelias GE. 1990. Production and repair of chromosome damage in an X-ray sensitive CHO mutant visualized and analysed in interphase using the technique of premature chromosome condensation. International Journal of Radiation Biology 57: 1213-1223.
16. International Atomic Energy Agency. 1986. Biological Dosimetry: Chromosome Aberration Analysis for Dose Assessment. Vienna: International Atomic Energy Agency.
17. International Atomic Energy Agency. 1990. The radiological accidents in San Salvador. Vienna: International Atomic Energy Agency.
18. International Atomic Energy Agency. 1993. The radiological accidents in Soreq. Vienna: International Atomic Energy Agency.
19. International Atomic Energy Agency. 1996. The radiological accidents at the irradiation facility in Nesvizh. Vienna: International Atomic Energy Agency.
20. Ito S, Gotoh E, Ozawa S, Yanagi K. 2002. Epstein-Barr virus nuclear antigen-1 is highly colocalized with interphase chromatin and its newly replicated regions in particular. Journal of General Virology 83: 2377-2388.
21. Johnson RT, Rao PN. 1970. Mammalian cell fusion: induction of premature chromosome condensation in interphase nuclei. Nature 226: 717-722.
22. Johnson RT, Gotoh E, Mullinger AM, Ryan AJ, Shiloh Y, Ziv Y, Squires S. 1999. Targeting double-strand breaks to replicating DNA identifies a subpathway of DSB repair that is defective in ataxia-telangiectasia cells. Biochemistry and Biophysics Research Communications 261: 317-325.
23. Kanda R, Hayata I, Lloyd DC. 1999. Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes. International Journal of Radiation Biology 75: 441-446.
24. Lloyd DC. 1984. An overview of radiation dosimetry by conventional cytogenetic methods. In: Eisert WG, Mendelsohn ML, editors. Biological dosimetry. Berlin: Springer, p. 3-14.
25. Lloyd DC, Edwards AA. 1990. Biological dosimetry and radiation accidents. In: Obe G, Natarajan AT, editors. Chromosomal aberrations. Berlin: Springer, p. 212-223.
26. Lloyd DC. 1997. Chromosomal analysis to assess radiation dose. Stem Cells 15(Suppl. 2): 195-201.
27. Natarajan AT, Vyas RC, Wiegant J, Curado MP. 1991. A cytogenetic follow-up study of the victims of a radiation accident in Goiania (Brazil). Mutation Research 247: 103-111.
28. Natarajan AT, Vyas RC, Darroudi F, Vermeulen S. 1992. Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by in situ hybridization using chromosome-specific DNA libraries. International Journal of Radiation Biology 61: 199-203.
29. Prasanna PG, Escalada ND, Blakely WF. 2000. Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes: a simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridization probes for biological dosimetry. Mutation Research 466: 131-141.
30. Prasanna PG, Hamel CJ, Escalada ND, Duffy KL, Blakely WF. 2002. Biological dosimetry using human interphase peripheral blood lymphocytes. Military Medicine 167: 10-12.
31. Waldren CA, Johnson RT. 1974. Analysis of interphase chromosome damage by means of premature chromosome condensation after X- and ultraviolet- irradiation. Proceedings of National Academy of Sciences, USA 71: 1137-1141.