In vitro bioassay of endotoxin using fluorescein as a pH indicator in a macrophage cell culture system

Yonsei Medical Journal

Volumn 46, Issue 2, 2005, Pages 268-274

  Lee, Dong Hee ^a   Sung, Hak Joon ^a   Han, Dong Wook ^a   Lee, Min Sub ^a   Ryu, Gyu Ha ^b   Aihara, Maki ^c   Takatori, Kosuke ^c   Park, Jong Chul ^a  

^a Yonsei University College of Medicine   (South Korea)

^b Korea Food and Drug Administration   (South Korea)

^c NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Fluorescein; Limulus Amebocyte Lysate test; Lipopolysaccharide; Macrophage; pH; Staphylococcus aureus

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; ENDOTOXIN; FLUORESCEIN; LIPOPOLYSACCHARIDE;

ANIMAL CELL; ARTICLE; BIOASSAY; CELL CULTURE; CELL LINE; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; ENDOTOXIN DETECTION; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; IN VITRO STUDY; INTERMETHOD COMPARISON; LIMULUS LYSATE TEST; MACROPHAGE; MOUSE; NONHUMAN; PSEUDOMONAS AERUGINOSA; SALMONELLA MINNESOTA; STAPHYLOCOCCUS AUREUS;

EID: 18644381188     PISSN: 05135796     EISSN: None     Source Type: Journal    
DOI: 10.3349/ymj.2005.46.2.268     Document Type: Article

References (24)

1. Ulevitch RJ, Tobias PS. Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu Rev Immunol 1995;13:437-57.
2. Iwanaga S, Morita T, Harada T, Nakamura S, Niwa M, Takada K, et al. Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins. Haemostasis 1978;7:183-8.
3. Iwanaga S. The limulus clotting reaction. Curr Opin Immunol 1993;5:74-82.
4. Rietschel ET, Brade H, Brade L, Brandenburg K, Schade U, Seydel U, et al. Lipid A, the endotoxic center of bacterial lipopolysaccharides: relation of chemical structure to biological activity. Prog Clin Biol Res 1987;231:25-53.
5. Laude-Sharp M, Haeffner-Cavaillon N, Caroff M, Lantreibecq F, Pusineri C, Kazatchkine MD. Dissociation between the interleukin 1-inducing capacity and Limulus reactivity of lipopolysaccharides from Gram-negative bacteria. Cytokine 1990;2:253-8.
6. Eperon S, De Groote D, Werner-Felmayer G, Jungi TW. Human monocytoid cell lines as indicators of endotoxin: comparison with rabbit pyrogen and Limulus amoebocyte lysate assay. J Immunol Methods 1997;207:135-45.
7. Werner-Felmayer G, Baier-Bitterlich G, Fuchs D, Hausen A, Murr C, Reibnegger G, et al. Detection of bacterial pyrogens on the basis of their effects on gamma interferon-mediated formation of neopterin or nitrite in cultured monocyte cell lines. Clin Diagn Lab Immunol 1995;2:307-13.
8. Jungi TW. A turbidimetric assay in an ELISA reader for the determination of mononuclear phagocyte procoagulant activity. J Immunol Methods 1990;133:21-9.
9. McCoy KL. Contribution of endosomal acidification to antigen processing. Semin Immunol 1990;2:239-46.
10. Mellman I, Fuchs R, Helenius A. Acidification of the endocytic and exocytic pathways. Annu Rev Biochem 1986;55:663-700.
11. Sjoback R, Nygren J, Kubista M. Absorption and Fluorescence Properties of Fluorescein. Spectrochim Acta A 1995;51:7-12.
12. Cooper JF. Validation of bacterial endotoxin test methods. LAL Times 1999;6:1-4.
13. FDA Interim Guidance (1991). U.S.FDA, Guideline on validation of the Limulus amebocyte lysate test as an in-process endotoxin test for human and animal parenteral drugs, biological products and medical devices, DHHS, Dec. 1987 and Interim Guidance, 1991.
14. Levin J, Tomasulo PA, Oser RS. Detection of endotoxin in human blood and demonstration of an inhibitor. J Lab Clin Med 1970;75:903-11.
15. Takagi K, Moriya A, Tamura H, Nakahara C, Tanaka S, Fujita V, et al. Quantitative measurement of endotoxin in human blood using synthetic chromogenic substrate for horseshoe crab clotting enzyme: a comparison of methods of blood sampling and treatment. Thromb Res 1981;23:51-7.
16. Peterson AA, Muriford RS. Dephosphorylation of the lipid A moiety of Escherichia coli lipopolysaccharide by mouse macrophages. Infect Immun 1987;55:974-8.
17. Ulevitch RJ. Interaction of bacterial lipopolysaccharide and plasma high density lipoproteins. In: Berry LJ, editor. Handbook of endotoxin. Amsterdam: Elsvier; 1985. p.372-88.
18. Urbaschek B, Becker KP, Ditter B, Urbaschek R. Quantification of endotoxin and sample-related interferences in human plasma and cerebrospinal fluid by using a kinetic Limulus amoebocyte lysate microtiter test. In: Leive L, editor. Microbiology. Washington DC: American Society for Microbiology; 1985. p.39-42.
19. Eperon S, Jungi TW. The use of human monocytoid lines as indicators of endotoxin. J Immunol Methods 1996;194: 121-9.
20. Kreutz M, Ackermann U, Hauschildt S, Krause SW, Riedel D, Bessler W, et al. A comparative analysis of cytokine production and tolerance induction by bacterial lipopeptides, lipopolysaccharides and Staphylococcus aureus in human monocytes. Immunology 1997;92:396-401.
21. Johannsen L, Obal F Jr, Kapas L, Kovalzon V, Krueger JM. Somnogenic activity of muramyl peptide-derived immune adjuvants. Int J Immunopharmacol 1994;16: 109-16.
22. Himanen JP, Pyhala L, Olander RM, Merimskaya O, Kuzina T, Lysyuk O, et al. Biological activities of lipoteichoic acid and peptidoglycan-teichoic acid of Bacillus subtilis 168 (Marburg). J Gen Microbiol 1993;139:2659-65.
23. Freudenberg MA, Galanos C. Tumor necrosis factor alpha mediates lethal activity of killed Gram-negative and Gram-positive bacteria in D-galactosamine-treated mice. Infect Immun 1991;59:2110-5.
24. Moesby L, Jensen S, Hansen EW, Christensen JD. A comparative study of Mono Mac 6 cells, isolated mononuclear cells and Limulus amoebocyte lysate assay in pyrogen testing. Int J Pharm 1999;191:141-9.