A novel procedure for separating small peptides on polyacrylamide gels

Letters in Peptide Science

Volumn 10, Issue 2, 2003, Pages 127-133

  Sarfo, Kwabena ^a   Moorhead, Greg B G ^a   Turner, Raymond J ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Detergent binding; PAGE; SDS

Indexed keywords

AMINO ACID; ANAZOLENE SODIUM; PEPTIDE; POLYACRYLAMIDE;

ARTICLE; COMPLEX FORMATION; ELECTROPHORESIS; HYDROPHILICITY; HYDROPHOBICITY; ISOELECTRIC POINT; MIGRATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEPARATION TECHNIQUE; STAINING;

EID: 18544379004     PISSN: 09295666     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:LIPS.0000032383.40916.e2     Document Type: Article

References (12)

1. Andrews, A. T., Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, Clarendon Press, Oxford, 1986.
2. Lee, H.-J. and Chang, C., Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter. J. Biol. Chem., 270 (1995) 5434.
3. Stueber, D., Matile H. and Garotta, G., System for high-level production in Escherichia coli and rapid purification of recombinant proteins: Application to epitope mapping, preparation of antibodies and structure-function analysis. Immunol. Methods, 4 (1990) 121.
4. Atherton, E., Fox, H., Harkis, C. J., Logan, R. C., Sheppard, B. J. and Williams, A., A mild procedure for solid phase peptide synthesis: use of fluorenylmethoxycarbonylamino-acids. J. Chem. Soc. Chem. Comm., (1978) 537-539.
5. Lutz, M. P., Pines, D. I. and Miller, L. J., A nonradioactive fluorescent gel-shift assay for the analysis of protein phosphatase and kinase activities toward protein-specific peptide substrates. Anal. Biochem., 220 (1994) 268.
6. Reynolds, J. A. and Tanford, C., Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes. Proc. Natl. Acad. Sci., 66 (1970) 1002.
7. Schaegger, H. and Von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem., 166 (1987) 368.
8. Selsted, M. E. and Becker, H. W., Eosin Y: A reversible stain for detecting electrophoretically resolved protein. Anal. Biochem., 155 (1986) 270.
9. Selsted, M. E., Brown, D. M., Delange, R. J., Harwig, S. S. L. and Lehrer, R. I., Primary structures of six antimicrobial peptides of rabbit peritoneal neutrophils. J. Biol. Chem., 260 (1985) 4579.
10. Takagi, T., Tsujii, K. and Shirahama, K., Binding isotherms of sodium dodecyl sulfate to protein polypeptides with special reference to SDS-polyacrylamide gel electrophoresis. J. Biochem., 77 (1975) 939.
11. Tachi, T., Epand, R. F. Epand, R. M. and Matsuzaki, K., Position-dependent hydrophobicity of the antimicrobial magainin peptide affect the mode of peptide-lipid interactions and selective toxicity. Biochemistry, 41 (2002) 10723.
12. Samso, M., Daban, J.-R., Hansen, S. and Jones, G. R., Evidence for sodium dodecyl sulfate/protein complexes adopting a necklace structure. Eur. J. Biochem., 232 (1995) 818.