Quantification of soybean phospholipids in soybean degummed oil residue by HPLC with evaporative light scattering detection

Journal of Liquid Chromatography and Related Technologies

Volumn 28, Issue 9, 2005, Pages 1333-1343

  Zhang, Yingying ^a   Yang, Yiwen ^a   Ren, Qilong ^a   Jiang, Hailiang ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

ELSD; HPLC; Phospholipid; Quantification; Soybean degummed oil residue

Indexed keywords

DETECTORS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGHT SCATTERING; LIPIDS; PHOSPHOLIPIDS; SILICA GEL; VEGETABLE OILS; WATER;

GLYCOLIPIDS (GL); NEUTRAL LIPIDS (NL); QUANTIFICATION; SOYBEAN PHOSPHOLIPIDS; STATIONARY PHASES;

FOOD PRODUCTS;

2 PROPANOL; GLYCOLIPID; HEXANE; LYSOPHOSPHATIDYLCHOLINE; PHOSPHATIDIC ACID; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLINOSITOL; PHOSPHOLIPID; SILICA GEL; SPHINGOMYELIN;

ACCURACY; ARTICLE; EVAPORATIVE LIGHT SCATTERING DETECTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGHT SCATTERING; PHASE SEPARATION; REFRACTION INDEX; SOYBEAN;

GLYCINE MAX;

EID: 18444418039     PISSN: 10826076     EISSN: None     Source Type: Journal    
DOI: 10.1081/JLC-200054817     Document Type: Article

References (15)

1. Helmerich, G.; Koehler, P. Comparison of methods for the quantitative determination of phospholipids in lecithins and flour improvers. J. Agric. Food Chem. 2003, 51, 6645-6651.
2. Schariter, J.A.; Pachuski, J.; Fried, B.; Sherma, J. Determination of neutral lipids and phospholipids in the cercariae of schistosoma mansoni by high performance thin layer chromatography. J. Liq. Chromatogr. & Rel. Technol. 2002, 25 (10-11), 1615-1622.
3. Yang, Y.; Li, Y.; Wu, C.; Ren, Q.; Wu, P. Determination of soybean phosphatidylcholine by HPLC-RI. J. Instrum. Anal. 2004, 23 (5), 118-121.
4. Yoon, T.H.; Kim, I.H. Phosphatidylcholine isolation from egg yolk phospholipids by high-performance liquid chromatography. J. Chromatogr. A 2002, 949, 209-216.
5. Kang, D.H.; Row, K.H. Fractionation of soybean phospholipids by preparative high-performance liquid chromatography with sorbents of various particle size. J. Chromatogr. A 2002, 949, 217-223.
6. Nomikos, T.; Karantonis, H.C.; Fragopoulou, E.; Demopoulos, C.A. One-step separation system for the main phospholipids, glycolipids, and phenolics by normal phase HPLC. Application to polar lipid extracts from olive and sunflower oils. J. Liq. Chromatogr. & Rel. Technol. 2002, 25 (1), 137-149.
7. Zhang, W.; He, H.; Feng, Y.; Da, S. Separation and purification of phosphatidylcholine and phosphatidylethanolamine from soybean degummed oil residues by using solvent extraction and column chromatography. J. Chromatogr. B 2003, 798, 323-331.
8. Grizard, G.; Sion, B.; Bauchart, D.; Boucher, D. Separation and quantification of cholesterol and major phospholipid classes in human semen by high-performance liquid chromatography and light-scattering detection. J. Chromatogr. B 2000, 740, 101-107.
9. Bao, Y.P.; Hurst, R.; Williamson, G. High performance liquid chromatographic separation of hydroperoxy-phospholipids and their corresponding hydroxyphospholipid derivatives. J. Liq. Chromatogr. & Rel. Technol. 1998, 21 (13), 2061-2068.
10. Oppenheimer, L.E.; Mourey, T.H. Examination of the concentration response of evaporative light-scattering mass detectors. J. Chromatogr. 1985, 323, 297-304.
11. Meeren, P.; Van Der; Vanderdeelen, J.; Huys, M.; Baert, L. Simple and rapid method for high-performance liquid chromatographic separation and quantification of soybean phospholipids. J. Chromatogr. B 1988, 447, 436-442.
12. Vaghela, M.N.; Kilara, A. Quantitative analysis of phospholipids from whey protein concentrates by high-performance liquid chromatography with a narrow-bore column and an evaporative light-scattering detector. JAOCS 1995, 72 (6), 729-733.
13. Row, K.H.; Kang, D.H. Normal-phase high-performance liquid chromatography of phospholipids from soybean with evaporative light scattering detection. Am. Bio-technol. Lab. 2003, August, 40-42.
14. Caboni, M.F.; Menotta, S.; Lercker, G. High-performance liquid chromatography separation and light-scattering detection of phospholipids from cooked beef. J. Chromatogr. A 1994, 683, 59-65.
15. Matsuda, K.; Matsuda, S.; Saito, M.; Ito, Y. Separation of phospholipids and glycolipids using analytical toroidal-coil countercurrent chromatography. I. Separation of human brain lipids. J. Liq. Chromatogr. & Rel. Technol. 2002, 25 (8), 1255-1269.