SEM examination of human erythrocytes in uncoated bloodstains on stone: Use of conventional as environmental-like SEM in a soft biological tissue (and hard inorganic material)

Journal of Microscopy

Volumn 218, Issue 2, 2005, Pages 94-103

  Hortolà, Policarp ^a  

^a UNIVERSITAT ROVIRA I VIRGILI   (Spain)

Author keywords

Blood smears; Haemotaphonomy; Red blood cells; Scanning electron microscopy; Specimen preparation

Indexed keywords

BLOOD; CELLS; COATINGS; ELECTRONS; LIME; METAL SUBSTRATES; SECONDARY EMISSION; SPECIMEN PREPARATION;

BLOOD SMEARS; CARBON COATING; ENVIRONMENTAL SCANNING ELECTRON MICROSCOPES; HAEMOTAPHONOMY; HUMAN ERYTHROCYTES; INORGANIC MATERIALS; RED BLOOD CELL; SCANNING ELECTRONS; SECONDARY ELECTRONS; SOFT BIOLOGICAL TISSUE;

SCANNING ELECTRON MICROSCOPY;

ACID; ALKALI; CARBON; INORGANIC COMPOUND; LIMESTONE; METAL;

ADULT; ARTICLE; BLOOD SAMPLING; BLOOD SMEAR; BLOOD STAIN; ERYTHROCYTE; ERYTHROCYTE STRUCTURE; FEMALE; HUMAN; MALE; MATERIAL COATING; NORMAL HUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; ROCK; SAMPLE; SCANNING ELECTRON MICROSCOPE; SCANNING ELECTRON MICROSCOPY; TECHNIQUE;

EID: 18444405842     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2005.01477.x     Document Type: Article

References (21)

1. Barnhart, M.I., Wallace, M.A. & Lusher, J.M. (1983) Red blood cells. Biomedical Research Applications of Scanning Electron Microscopy, Vol. 3 (ed. by G. M. Hodges and K. E. Carr), pp. 171-243. Academic Press, London.
2. Bessis, M. (1974) Corpuscles. Atlas of Red Blood Cell Shapes. Springer, Berlin.
3. Bull., B.S. & Breton-Gorius, J. (1995) Morphology of the erythron. Williams Hematology, 5th edn (ed. by E. Beutler, M. A. Lichtman, B. S. Coller and T. J. Kipps), pp. 349-363. McGraw-Hill, New York.
4. Castoldi, G.L. (1981) Erythrocytes. Atlas of Blood Cells. Function and Pathology (ed. by D. Zucker-Franklin, M. F. Greaves, C. E. Grossi and A. M. Marmont), pp. 35-145. Ermes/Lea & Febiger, Milan/Philadelphia.
5. Fawcett, D.W. & Raviola, E. (1994) Bloom and Fawcett, a Textbook of Histology, 12th edn. Chapman & Hall, New York.
6. Fiori, A. (1962) Detection and identification of bloodstains. Methods of Forensic Science. Vol. 1 (ed. by F. Lundquist), pp. 243-290. John Wiley & Sons, New York.
7. Geyh, M.A. & Schleicher, H. (1990) Absolute Age Determination. Physical and Chemical Dating Methods and Their Application. Springer-Verlag, Berlin.
8. Goldstein, J.I., Newbury, D.E., Echlin, P., Joy, D.C., Romig, A.D. Jr, Lyman, C.E., Fiori, C. & Lifshin, E. (1992) Scanning Electron Microscopy and X-Ray Microanalysis. A Text for Biologists, Materials Scientists, and Geologists, 2nd edn. Plenum Press, New York.
9. Handley, C.O. Jr & Ponder, E. (1961) Erythrocyte diameters: mammals. Blood and Other Body Fluids (ed. by D. S. Dittmer), pp. 119-120. Federation of American Societies for Experimental Biology, Washington D.C.
10. Hortolà, P. (1992a) SEM analysis of red blood cells in aged human bloodstains. Forensic Sci. Int. 55, 139-159.
11. Hortolà, P. (1992b) SEM characterization of blood stains on stone tools. The Microscope 40, 111-113 (Errata, 40(3), vi).
12. Hortolà, P. (1994) Application of SEM to the study of red blood cells in forensic bloodstains. Microsc. Anal. (UK) 40, 19-21 and
13. Microsc. Anal (EU), 28, 21-23.
14. Hortolà, P. (2001a) Experimental SEM determination of game mammalian bloodstains on stone tools. Environ. Archaeol. 6, 99-104.
15. Hortolà, P. (2001b) Morphological characterisation of red blood cells in human bloodstains on stone: a systematical SEM study. Anthropologie 39, 235-240.
16. Hortolà, P. (2002) Red blood cell haemotaphonomy of experimental human bloodstains on techno-prehistoric lithic raw materials. J. Archaeol. Sci. 29, 733-739.
17. Jain, N.C. (1986) Schalm's Veterinary Hematology, 4th edn. Lea & Febiger, Philadelphia.
18. LeBlond, P.F. & Shoucri, R. (1978) Calculation of surface area and volume of human erythrocytes from scanning electron micrographs. J. Microsc. 113, 161-170.
19. Loy, T.H. (1998) Organic residues on Oldowan tools from Sterkfontein Cave, South Africa. Dual Congress of the International Association for the Study of Human Paleontology, and International Association of Human Biologists (ed. by M. A. Raath, H. Soodyall, K. L. K. D. Barkhan and P. V. Tobias), pp. 74-75. University of the Witwatersrand Medical School, Johannesburg.
20. Miki, T., Kai, A. & Ikeya, M. (1987) Electron spin resonance of bloodstains and its application to the estimation of time after bleeding. Forensic Sci. Int. 35, 149-158.
21. Rozman, C., Woessner, S., Feliu, E., Lafuente, R. & Berga, L. (1993) Cell Ultrastructure for Hematologists. Doyma, Barcelona. 104-114