Conjugal vectors for cloning, expression, and insertional mutagenesis in Gram-negative bacteria

BioTechniques

Volumn 38, Issue 5, 2005, Pages 708-712

  Lauro, Federico M ^a,b   Eloe, Emiley A ^a   Liverani, Nicoletta ^b   Bertoloni, Giulio ^b   Bartlett, Douglas H ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF PADOVA   (Italy)

^c UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; GREEN FLUORESCENT PROTEIN; KANAMYCIN; PLASMID VECTOR; TETRACYCLINE;

ARTICLE; BACTERIAL STRAIN; CLONING VECTOR; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE DISRUPTION; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETIC COMPLEMENTATION; GRAM NEGATIVE BACTERIUM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOBACTERIUM PHOSPHOREUM; RECOMBINANT PLASMID; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PHOTOBACTERIUM PHOSPHOREUM;

EID: 18444397469     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05385BM06     Document Type: Article

References (17)

1. Knuth, K., H. Niesalla, C.J. Hueck, and T.M. Fuchs. 2004. Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol. Microbiol. 51:1729-1744.
2. Merlin, C., S. McAteer, and M. Masters. 2002. Tools for characterization of Escherichia coli genes of unknown function. J. Bacteriol. 184:4573-4581.
3. Kobayashi, K., S.D. Ehrlich, A. Albertini, G. Amati, K.K. Andersen, M. Arnaud, K. Asai, S. Ashikaga, et al. 2003. Essential Bacillus subtilis genes. Proc. Natl. Acad. Sci. USA 100:4678-4683.
4. Que, Q.Q. and E.A. Winzeler. 2002. Large-scale mutagenesis and functional genomics in yeast. Funct. Integr. Genomics 2:193-198.
5. Vezzi, A., S. Campanaro, M. D'Angelo, F. Simonato, N. Vitulo, F.M. Lauro, A. Cestaro, G. Malacrida, et al. 2005. Life at depth: Photobacterium profundum genome sequence and expression analysis. Science 307:1459-1461.
6. Allen, E.E., D. Facciotti, and D.H. Bartlett. 1999. Monounsaturated but not polyunsaturated fatty acids are required for growth of the deep-sea bacterium Photobacterium profundum SS9 at high pressure and low temperature. Appl. Environ. Microbiol. 65:1710-1720.
7. Welch, T.J. and D.H. Bartlett. 1998. Identification of a regulatory protein required for pressure-responsive gene expression in the deep-sea bacterium Photobacterium species strain SS9. Mol. Microbiol. 27:977-985.
8. Zhou, M.Y. and C.E. Gomez-Sanchez. 2000. Universal TA cloning. Curr. Issues Mol. Biol. 2:1-7.
9. Kovach, M.E., R.W. Phillips, P.H. Elzer, R.M. Roop II, and K.M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 16:800-802.
10. Kovach, M.E., P.H. Elzer, D.S. Hill, G.T. Robertson, M.A. Farris, R.M. Roop II, and K.M. Peterson. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176.
11. Brahamsha, B. 1996. A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus. Appl. Environ. Microbiol. 62:1747-1751.
12. Better, M. and D.R. Helinski. 1983. Isolation and characterization of the recA gene of Rhizobium meliloti. J. Bacteriol. 155:311-316.
13. Chi, E. and D.H. Bartlett. 1995. An rpoE-like locus controls outer membrane protein synthesis and growth at cold temperatures and high pressures in the deep-sea bacterium Photobacterium sp. strain SS9. Mol. Microbiol. 17:713-726.
14. Bagdasarian, M., R. Lurz, B. Ruckert, F.C. Franklin, M.M. Bagdasarian, J. Frey, and K.N. Timmis. 1981. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16:237-247.
15. Newman, J.R. and C. Fuqua. 1999. Broadhost-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator. Gene 227:197-203.
16. Hochhut, B., J. Marrero, and M.K. Waldor. 2000. Mobilization of plasmids and chromosomal DNA mediated by the SXT element, a constin found in Vibrio cholerae O139. J. Bacteriol. 182:2043-2047.
17. Miller, J.H. 1972. Experiments in Molecular Genetics. CSH Laboratory Press, Cold Spring Harbor, NY.