An X-ray microscopy perspective on the effect of glutaraldehyde fixation on cells

Journal of Microscopy

Volumn 218, Issue 2, 2005, Pages 185-192

  Jearanaikoon, S ^a   Abraham Peskir, J V ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Algal cell; Glutaraldehyde fixation; X ray microscopy

Indexed keywords

ALGAE; CYTOLOGY; MOLECULES; PROTEINS; RADIATION DAMAGE; X RAY ABSORPTION; X RAY MICROSCOPES;

ALGAL CELLS; CELL-BE; CELL/B.E; CELL/BE; GLUTARALDEHYDE FIXATION; GLUTARALDEHYDES; HIGH RESOLUTION; MEAN DIAMETER; MICROSCOPY TECHNIQUE; X RAY MICROSCOPY;

CELLS;

GLUTARALDEHYDE; WATER;

ALGA; ANABAENA SPIROIDES; ARTICLE; ARTIFACT; CELL ULTRASTRUCTURE; CHLORELLA VULGARIS; CONTROLLED STUDY; ELECTRON MICROSCOPY; IMAGE ANALYSIS; IMAGE QUALITY; IMAGING SYSTEM; MICROCYSTIS AERUGINOSA; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; RADIATION INJURY; ROENTGEN MICROSCOPY; STRUCTURE ANALYSIS; TECHNIQUE; TISSUE FIXATION;

ALGAE; ANABAENA SPIROIDES; CHLORELLA VULGARIS; MICROCYSTIS AERUGINOSA;

EID: 18444394942     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2005.01472.x     Document Type: Article

References (21)

1. Abraham-Peskir, J.V. (1998) Structural changes in fully hydrated Chilomonas paramecium exposed to copper. Eur. J. Protistol. 34, 51-57.
2. Abraham-Peskir, J.V. (2000) X-ray microscopy with synchrotron radiation: applications to cellular biology. Cell. Mol. Biol. 46, 1045-1052.
3. Abraham-Peskir, J.V., Chantler, E., McCann, C., Medenwaldt, R. & Ernst, E. (1998) Ultrastructure of human sperm using X-ray microscopy. Med. Sci. Res. 22, 663-667.
4. Abraham-Peskir, J.V., Chantler, E., Uggerhøj, E. & Fedder, J. (2002) Response of midpiece vesicles on human sperm to osmotic stress. Hum. Reprod. 17, 375-382.
5. Balhorn, R., Corzett, M., Allen, M.J., et al. (1992) Application of X-rays to the analysis of DNA packaging in mammalian sperm. Soft X-Ray Microscopy SPIE, 1741, 374-385.
6. Bozzola, J.J. & Russell, L.D. (1999) Electron Microscopy: Principles and Techniques for Biologists. 2nd edn. Jones and Bartlett Publishers, Massachusetts.
7. UKNCC (2001) Catalogue of the UK National Culture Collection: List of Algae and Protozoa, 1st edn. Pineapple Planet Ltd. Swindon.
8. Cheng, P.C. (1987) Sample preparation for X-ray imaging and examples of biological X-ray Images. X-Ray Microscopy: Instrumentation and Biological Applications (ed. by P. C. Cheng and G. J. Jan), pp. 289-310. Springer-Verlag, Berlin.
9. Ford, T.W., Cotton, R.A., Page, A.M., Meyer-Ilse, W., Brown, J.T., Heck, J. & Stead, A.D. (1995) A comparative study of the ultrastructure of living cells of the green alga Chlamydomonas using both soft X-ray contact and direct imaging systems and an evaluation of possible radiation damage. X-Ray Microscopy and Spectromicroscopy II (ed. by D. Rudolph, E. Umbach, J. Thieme and G. Schmahl), pp. 185-189. Springer-Verlag, Berlin.
10. Magowan, C., Brown, J.T., Liang, J., Heck, J., Coppel, R.L., Mohandas, N. & Meyer-Ilse, W. (1997) Intracellular structures of normal and aberrant Plasmodium falciparum malaria parasites imaged by soft X-ray microscopy. Proc. Natl Acad. Sci. USA, 94, 6222-6227.
11. Maser, J., Osanna, A., Wang, Y., et al. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope. I. Instrumentation, imaging and spectroscopy. J. Microsc. 197, 68-79.
12. Medenwaldt, R. & Uggerhøj, E. (1998) Description of an X-ray microscope with 30 nm resolution. Rev. Sci. Instrum. 69, 2974-2977.
13. Sayre, D., Kirtz, J., Feder, R., Kim, D.M. & Spiller, E. (1977) Transmission microscopy of unmodified biological materials: comparative radiation dosages with electrons and ultra soft X-ray photons. Ultramicroscopy. 2, 337-349.
14. Scharf, J.G. & Schneider, G. (1999) Ultrastructural characterization of isolated rat Kupffer cells by transmission X-ray microscopy. J. Microsc. 193, 250-256.
15. Schneider, G. (1998) Cryo X-ray microscopy with high spatial resolution in amplitude and phase contrast. Ultramicroscopy, 75, 85-104.
16. Vonshak, A. (1986) Laboratory techniques for the cultivation of microalgae. Handbook of Microalgal Mass Culture (ed. by A. Richmond), pp. 117-147. CRC Press, Florida.
17. Vorup-Jensen, T., Hjort, T., Abraham-Peskir, J., Guttmann, P., Jensenius, J.C., Uggerhøj, E, & Medenwaldt, R. (1999) X-ray microscopy of human spermatozoa shows change of mitochondrial morphology after capacitation. Hum. Reprod. 14, 880-884.
18. Wang, Y., Jacobsen, C., Maser, J. & Osanna, A. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope. II. Tomography. J. Microsc. 197, 80-93.
19. 2+-induced structural changes and localization in a fully-hydrated live eukaryotic cellular model. Cell Biol. Int. 25, 521-530.
20. William, S., Zhang, X., Jacobsen, C., Kirz, J., Lindass, S., Van'tHof, J. & Lamm, S.S. (1993) Measurements of wet metaphase chromosomes in the scanning transmission X-ray microscope. J. Microsc. 170, 155-165.
21. Yamamoto, A., Masaki, R., Guttmann, P., Schmahl, G. & Kihara, H. (1998) Studies on intracellular structures of COS cells by X-ray microscopy. J. Synchrotron Radiat. 5, 1105-1107.