Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by BHK cells

Journal of Biotechnology

Volumn 96, Issue 2, 2002, Pages 169-183

  Cruz, H J ^a,d,e   Conradt, H S ^b   Dunker, R ^c   Peixoto, C M ^a   Cunha, A E ^a   Thomaz, M ^a   Burger, C ^c   Dias, E M ^a   Clemente, J ^a   Moreira, J L ^a   Rieke, E ^c   Carrondo, M J T ^a,f  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c MERCK KGAA   (Germany)

^d ECBio R and D in Biotechnology   (Portugal)

^e Universidade Independente   (Portugal)

^f UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

BHK cells; EGF receptor; Pharmacokinetics; Purification; Recombinant fusion protein; Tumour immunotherapy; Tumour targeted therapy

Indexed keywords

ANTIBODIES; CHEMOSTATS; MASS SPECTROMETRY; PURIFICATION; TUMORS;

FUSION PROTEIN; GLYCOSYLATION; RECOMBINANT ANTIBODY;

BIOTECHNOLOGY;

HYBRID PROTEIN; RECOMBINANT ANTIBODY; RECOMBINANT INTERLEUKIN 2;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER THERAPY; CELL LINE; CELL STRAIN BHK; CHEMOSTAT; CONTROLLED STUDY; DRUG EFFECT; DRUG STRUCTURE; DRUG SYNTHESIS; GLYCOSYLATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN QUALITY; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL LINE; CELL-FREE SYSTEM; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAINS; INTERLEUKIN-2; KIDNEY; MICE; MICE, INBRED BALB C; MICE, NUDE; OLIGOSACCHARIDES; PERFUSION; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TUMOR CELLS, CULTURED;

EID: 18444382663     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(02)00028-7     Document Type: Article

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