Validation and use of an indirect enzyme-linked immunosorbent assay for detection of antibodies to West Nile virus in American alligators (Alligator mississippiensis) in Florida

Journal of Wildlife Diseases

Volumn 41, Issue 1, 2005, Pages 107-114

  Jacobson, Elliot R ^a   Johnson, April J ^a   Hernandez, Jorge A ^a   Tucker, Sylvia J ^a   Dupuis II, Alan P ^b   Stevens, Robert ^c   Carbonneau, Dwayne ^d   Stark, Lillian ^e  

^a University of Florida College of Veterinary Medicine   (United States)

^b WADSWORTH CENTER   (United States)

^c West Orange Veterinary Hospital   (United States)

^d FISH AND WILDLIFE RESEARCH INSTITUTE   (United States)

^e FLORIDA DEPARTMENT OF HEALTH   (United States)

Author keywords

Alligator mississippiensis; American alligator; ELISA; HAI; PRNT; Serology; West Nile virus

Indexed keywords

ALLIGATOR; ALLIGATOR MISSISSIPPIENSIS; ALLIGATORINAE; FLAVIVIRUS; ORYCTOLAGUS CUNICULUS; WEST NILE VIRUS;

EID: 18444367085     PISSN: 00903558     EISSN: None     Source Type: Journal    
DOI: 10.7589/0090-3558-41.1.107     Document Type: Article

References (12)

1. Beaty, B. J., C. H. Calisher, AND R. E. Shope. 1995. Arboviruses. In Diagnostic procedures for viral, rickettsial and chlamydial infections, 7th ed., E. H. Lennette, D. A. Lennette, and E. T. Lennette (eds.). American Public Health Association, Washington, D.C., pp. 189-212.
2. Blitvich, B. J., N. L. Marlenee, R. A. Hall, C. H. Caslisher, R. A. Bowen, J. T. Roehrig, N. Komar, S. A. Langevin, and B. J. Beaty. 2003. Epitope-blocking enzyme-linked immunosorbent assays for the detection of serum antibodies to West Nile virus in multiple avian species. Journal of Clinical Microbiology 41: 1041-1047.
3. Brown, D. R., I. M. Schumacher, M. F. Nogueira, L. J. Richey, A. Zacher, T. R. Schoeb, K. A. Vliet, R. A. Bennett, E. R. Jacobson, and M. B. Brown. 2001. Detection of antibodies to a pathogenic Mycoplasma in American alligators (Alligator missippiensis), broad-nosed caiman (Caiman latirostris), and Siamese crocodiles (Crocodylus siamensis). Journal of Clinical Microbiology 39: 285-292.
4. Ebel, G. D., A. P. Dupuis II, K. A. Ngo, D. C. Nicholas, E. B. Kauffman, S. A. Jones, D. Young, J. Maffei, P-Y. Shi, K. Bernard, and L. D. Kramer. 2001. Partial genetic characterization of West Nile virus strains, New York State, 2000. Emerging Infectious Diseases 7: 650-653.
5. Ebel, G. D., A. P. Dupuis II, D. C. Nicholas, D. Young, J. Maffei, and L. D. Kramer. 2002. Detection by enzyme-linked immunosorbent assay of antibodies to West Nile virus in birds. Emerging Infectious Diseases 8: 979-982.
6. Jacobson, E. R., P. E. Ginn, J. M. Troutman, L. Farina, L. Stark, K. Klenk, K. L. Burkhalter, and N. Komar. 2005. West Nile virus in farmed American alligators (Alligator missippiensis) in Florida. Journal of Wildlife Diseases 41: 96-106.
7. Johnson, A. J., S. Langevin, K. L. Wolff, and N. Komar. 2003. Detection of anti-West Nile virus immunoglobulin M in chicken serum by an enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 41: 2002-2007.
8. Martin, D. A., D. A. Muth, T. Brown, A. J. Johnson, N. Karabatsos, and J. T. Roehrig. 2000. Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections. Journal of Clinical Microbiology 38: 1823-1826.
9. Miller, D. L., M. J. Mauel, C. Baldwin, G. Burtle, D. Iingram, M. E. Hines II, and K. S. Frazier. 2003. West Nile virus in farmed alligators. Emerging Infectious Diseases 9: 794-799.
10. Olson, G. A., J. R. Hessler, and R. E. Faith. 1975. Techniques for blood collection and intravascular infusions of reptiles. Laboratory Animal Science 25: 783-786.
11. Shortridge, K. F., and A. Oya. 1984. Arboviruses. In Diseases of amphibians and reptiles, G. L. Hoff, F. L. Frye, and E. R. Jacobson (eds.). Plenum Press, New York, New York, pp. 107-148.
12. Steinman, A., C. Banet-Noach, S. Tal, O. Levi, L. Simanov, S. Perk, M. Malkinson, and N. Shpigel. 2003. West Nile virus infection in crocodiles. Emerging Infectious Diseases 9: 887-889.