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1
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84887121261
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Fortunately, as we will see in this chapter, it is possible to define a set of conditions under which it is possible to recover the three-dimensional structure of the specimen from a set of these micrographs
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Fortunately, as we will see in this chapter, it is possible to define a set of conditions under which it is possible to recover the three-dimensional structure of the specimen from a set of these micrographs.
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2
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84887081368
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As stated in the previous paragraphs, amplitude contrast in TEM is not due to electron absorption but to elimination of high angle scattered electrons
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As stated in the previous paragraphs, amplitude contrast in TEM is not due to electron absorption but to elimination of high angle scattered electrons.
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3
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84887055850
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The central section theorem states that the two dimensional Fourier transform of a plane projection of a three dimensional density distribution is equal to the corresponding central section of the three dimensional transform normal to the direction view
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The central section theorem states that the two dimensional Fourier transform of a plane projection of a three dimensional density distribution is equal to the corresponding central section of the three dimensional transform normal to the direction view.
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4
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84887060862
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It is not necessary to tilt the sample holder to obtain different views because the high symmetric particles, that are almost spherical (icosahedral, dodecahedral, etc.), lay on the support film presenting a width range of views
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It is not necessary to tilt the sample holder to obtain different views because the high symmetric particles, that are almost spherical (icosahedral, dodecahedral, etc.), lay on the support film presenting a width range of views.
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5
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84887099731
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The existence of common lines is a direct consequence of the central section theorem. As the 2-D Fourier transform of a view is a central plane of the 3-D Fourier transform of the reconstruction, every two 2-D Fourier transforms intersect, and therefore have a line with identical values (in the absence of noise). In the case of symmetrical particles the number of "common lines" is increased
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The existence of common lines is a direct consequence of the central section theorem. As the 2-D Fourier transform of a view is a central plane of the 3-D Fourier transform of the reconstruction, every two 2-D Fourier transforms intersect, and therefore have a line with identical values (in the absence of noise). In the case of symmetrical particles the number of "common lines" is increased.
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6
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84887041210
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The Fourier transform of an n-fold image presents n-fold symmetry, and therefore each common line is repeated n times
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The Fourier transform of an n-fold image presents n-fold symmetry, and therefore each common line is repeated n times.
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7
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84887122676
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A Dalton is the biochemical term for "atomic mass unit"
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A Dalton is the biochemical term for "atomic mass unit".
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8
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84887088399
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See section 3
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See section 3
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9
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84887052130
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Note that, in the negative staining images, protein masses appear white (transparent to electrons) while the background is dark (strong scattering material)
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Note that, in the negative staining images, protein masses appear white (transparent to electrons) while the background is dark (strong scattering material).
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