Development of a rapid, sensitive and specific diagnostic assay for fish Aquareovirus based on RT-PCR

Journal of Virological Methods

Volumn 118, Issue 2, 2004, Pages 111-122

  Seng, E K ^a   Fang, Q ^b   Lam, T J ^a   Sin, Y M ^a,c  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b WUHAN INSTITUTE OF VIROLOGY   (China)

^c Singapore Fish Breed I   (Singapore)

Author keywords

Aquareovirus; Degenerate primers; RT PCR; Threadfin reovirus

Indexed keywords

DOUBLE STRANDED RNA; NUCLEIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; AQUAREOVIRUS; ARTICLE; CELL CULTURE; CONTROLLED STUDY; CYPRINODONTIFORMES; DNA TEMPLATE; FEMALE; FISH; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REOVIRUS; SALMON; STRIPED BASS; VIRUS CARRIER; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS INFECTION; VIRUS STRAIN; VIRUS TITRATION;

ANIMALS; BASE SEQUENCE; CARPS; CELL LINE; FISH DISEASES; FISHES; MOLECULAR SEQUENCE DATA; POECILIA; REOVIRIDAE; REOVIRIDAE INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

ALECTIS CILIARIS; AQUAREOVIRUS; CTENOPHARYNGODON IDELLA; CYPRINUS CARPIO; GRASS CARP REOVIRUS; GUPPY REOVIRUS; MORONE SAXATILIS; ONCORHYNCHUS KETA; POECILIA; POECILIA RETICULATA; REOVIRUS SP.; STRIPED BASS REOVIRUS; THREADFIN REOVIRUS;

EID: 1842635323     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.01.023     Document Type: Article

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