Detection of hemoglobin-based oxygen carriers in human serum for doping analysis: Confirmation by size-exclusion HPLC

Clinical Chemistry

Volumn 50, Issue 4, 2004, Pages 723-731

  Varlet Marie, Emmanuelle ^a   Ashenden, Michael ^b   Lasne, Françoise ^c   Sicart, Marie Therese ^a   Marion, Benedicte ^a   De Ceaurriz, Jacques ^c   Audran, Michel ^a  

^a UNIV MONTPELLIER   (France)

^b Sci/Indust Against Blood Doping   (Australia)

^c National Anti Doping Laboratory   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DIASPIRIN CROSSLINKED HEMOGLOBIN; HAPTOGLOBIN; HEMOGLOBIN; HEMOGLOBIN BASED OXYGEN CARRIER; HEMOGLOBIN DERIVATIVE; HEMOSPAN; OXYGEN; OXYGLOBIN; PHP; POLYMERIZED HEMOGLOBIN; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CONTROLLED STUDY; DOPING; ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN EXPERIMENT; NORMAL HUMAN; OXYGEN TRANSPORT; PLASMA; SERUM;

ADULT; ANIMALS; BLOOD SUBSTITUTES; CATTLE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOPING IN SPORTS; DRUG STABILITY; DRUG STORAGE; HEMOGLOBINS; HUMANS; MALE; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE DETECTION;

BOVINAE;

EID: 1842608381     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2003.026591     Document Type: Article

References (14)

1. Chang TMS. Red blood cell substitutes. In: Contreras M, ed. Best practice and research: clinical haematology, Vol. 13 Orlando, FL: Harcourt Publishers, 2000:651-67.
2. Savltsky J, Doczi J, Black J, Arnold JD. A clinical safety trial of stroma-free hemoglobin. Clin Pharmacol 1978;23:73-80.
3. Chang TMS. Oxygen carriers. Curr Opin Investig Drugs 2002;3:1187-90.
4. Talarico TL, Guise KJ, Stacey CJ. Chemical characterization of pyridoxalated hemoglobin polyoxyethylene conjugate. Biochim Biophys Acta 2000;1476:53-65.
5. Lok C. Blood product from cattle wins approval for use in humans. Nature 2001;410:855.
6. Chang TMS. Blood substitutes: principles, methods, products and clinical trials, Vol. 1. Basel, Switzerland: Karger, 1997 (full text available for free online viewing at www.artcell.mcgill.ca).
7. Lasne F, Crépin N, Ashenden M, Audran M, De Ceaurriz J. Detection of hemoglobin-based oxygen carriers in human serum for doping analysis: screening by electrophoresis. Clin Chem 2004;50:410-5.
8. Lurie F, Jahr J, Driessen B. The novel HemoCue® plasma/low hemoglobin system accurately measures small concentrations of three different hemoglobin-based oxygen carriers in plasma: hemoglobin Glutamer-200 (bovine) (Oxyglobin®), hemoglobin Glutamer-250 (bovine) (Hemopure®), and hemoglobin-Raffimer (Hemolink™). Anesth Analg 2002;95:870-3.
9. Hughes GS, Francom SF, Antal EJ, Adams WJ, Locker PK, Yancey EP, et al. Hematologic effects of a novel hemoglobin-based oxygen carrier in normal male and female subjects. J Lab Clin Med 1995;126:444-51.
10. Thevis M, Ogorzalek-Loo RR, Loo JA, Shänzer W. Doping control analysis of bovine hemoglobin-based oxygen therapeutics in human plasma by LC-electrospray ionization-MS/MS. Anal Chem 2003;75:2955-61.
11. Alma C, Trout GJ, Woodland N, Kazlauskas R. The detection of haemoglobin-based oxygen carriers. In: Shänzer W, Geyer H, Gotzmann A, Mareck U, eds. Recent advances in doping analysis (10). Köln: Sport und Buch StrauB, 2002:169-77.
12. Trout GJ, Rogerson JH, Cawley AT, Alma CW. Development in sports drug testing. Aust J Chem 2003;56:175-80.
13. Gotzmann A, Voss S, Machnik M, Shänzer W. Detection of Hemopure® and hemoglobin In human plasma by HPLC/UV and different types of columns - an approach to screen for this substance in doping analysis. In: Shänzer W, Geyer H, Gotzmann A, Mareck U, eds. Recent advances in doping analysis (10). Köln: Sport und Buch StrauB, 2002:179-87.
14. International Olympic Committee. I.O.C. list of classes of prohibited substances and methods of doping. Lausanne, Switzerland: International Olympic Committee, 2003. http://multimedia.olympic.org/pdf/en_report_542.pdf.