Screening of Large Protein Libraries by the "Cell Immobilized on Adsorbed Bead" Approach

Biotechnology and Bioengineering

Volumn 86, Issue 2, 2004, Pages 196-200

  Freeman, Amihay ^a   Cohen Hadar, Noa ^a   Abramov, Simona ^a   Modai Hod, Ronit ^a   Dror, Yael ^a   Georgiou, George ^b  

^a TEL AVIV UNIVERSITY   (Israel)

^b The University of Texas at Austin   (United States)

Author keywords

Beads; Enzyme evolution; Immobilized cells; Protein libraries; Screening

Indexed keywords

ADSORPTION; CELL IMMOBILIZATION; CLONING; COLOR; ENZYMES; FLUORESCENCE; GROWTH KINETICS; MUTAGENESIS; SEPARATION;

ENZYMATIC ASSAYS; MUTANT LIBRARIES;

BIOMEDICAL ENGINEERING;

CHROMOGENIC SUBSTRATE; FLUOROGEN; GLASS; PEPTIDE LIBRARY; POLYACRYLAMIDE; PROTEIN LIBRARY; UNCLASSIFIED DRUG;

ADSORPTION; ADSORPTION KINETICS; ARTICLE; CELL GROWTH; CELL POPULATION; CELL SEPARATION; CELL SURFACE; COLONY FORMATION; CONTROLLED STUDY; DIFFUSION; ENZYME ASSAY; EQUILIBRIUM CONSTANT; FLOW KINETICS; FLUOROSCOPY; GENE EXPRESSION REGULATION; GENE MUTATION; GENETIC SCREENING; HYDROGEL; IMMOBILIZED CELL; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PHYSICAL CHEMISTRY; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SCREENING TEST; SIGNAL TRANSDUCTION; SOLID; STEREOMICROSCOPY;

ADSORPTION; BETA-LACTAMASES; CELLS, IMMOBILIZED; ESCHERICHIA COLI; FEASIBILITY STUDIES; FLOW CYTOMETRY; MICROSPHERES; PEPTIDE LIBRARY; PROTEINS;

EID: 1842578294     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10883     Document Type: Article

References (22)

1. Bornscheuer UT, Meyer HH, Altenbuchner J. 1999. Directed evolution of an esterase: Screening of enzyme libraries based on pH indicators and a growth assay. Bioorg Med Chem 7:2169-2173.
2. Cherry JR, Lasma MH, Schneider P, Vind J, Svendsen A, Jones A, Pedersen AH. 1999. Directed evolution of a fungal peroxidase. Nat Biotechnol 17:379-384.
3. Cohen N, Abramov S, Dror Y, Freeman A. 2001. In vitro enzyme evolution: The screening challenge of isolating the one in a million. TIBTECH 19:507-510.
4. Christians FC, Scapozza L, Crameri A, Folkers G, Stemmer PC. 1999. Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling. Nat Biotechnol 17:259-264.
5. Farinas ET, Butler T, Arnold FH. 2001. Directed enzyme evolution. Curr Opin Biotechnol 12:545-551.
6. Francisco JA, Earhart CF, Georgiou G. 1992. Transport and anchoring of β-lactamase to the external surface of Escherichia coli. Proc Natl Acad Sci 89:2713-2717.
7. Freeman A, Abramov S, Georgiou G. 1996. Fixation and stabilization of Escherichia coli cells displaying genetically engineered cell surface proteins. Biotechnol Bioeng 52:625-630.
8. Georgiou G, Stathopoulos C, Daugherty P, Nayak AR, Iverson BL, Curtiss R. 1997. Displaying of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15:29-34.
9. Giver L, Gershenson A, Freskgard PO, Arnold FH. 1998. Directed evolution of a thermostable esterase. Proc Natl Acad Sci 95:12809-12813.
10. Joo H, Lin Z, Arnold FH. 1999. Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation. Nature 399:670-673.
11. May O, Nguyen PT, Arnold FH. 2000. Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine. Nat Biotechnol 18:1071-1074.
12. Moore JC, Arnold FH. 1996. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat Biotechnol 14:458-467.
13. Olsen MJ, Daugherty P, Georgiou G, Iverson B. 2000a. Function-based isolation of novel enzymes from a large library. Nat Biotechnol 18: 1071-1074.
14. Olsen M, Iverson B, Georgiou G. 2000b. High-throughput screening of enzyme libraries. Curr Opin Biotechnol 11:331-337.
15. Reetz MT, Zonta A, Schimossek K, Liebeton K, Jaeger KE. 1997. Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution. Angew Chem Int Ed 36:2830-2832.
16. Reetz MT. 2002. New methods for high-throughput screening of enantioselective catalysts and biocatalysts. Angew Chem Int Ed 41:1335-1338.
17. Reetz MT, Jaeger KE. 2000. Superior biocatalysts by directed evolution. Top Curr Chem 200:1-57.
18. Reuveny S, Mizrahi A, Kotler M, Freeman A. 1983. Factors effecting cell attachment, spreading and growth on derivatized microcarriers. Biotechnol Bioeng 25:2969-2980.
19. Schmidt-Dannert C, Arnold FH. 1999. Directed evolution of industrial enzymes. TIBTECH 17:135-136.
20. Youvan DC, Goldman E, Delagrave S, Yang MM. 1995. Digital imaging spectroscopy for massively parallel screening of mutants. Meth Enzymol 246:732-752.
21. Wahler D, Reymond JL. 2001. Novel methods for biocatalyst screening. Curr Opin Chem Biol 5:152-158.
22. Zhang JH, Dawest G, Stemmer WPC. 1997. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc Natl Acad Sci 94:4504-4509.