An Effective Elimination of False Positives Isolated from Differential Display of mRNAs

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 6, Issue 2, 1996, Pages 213-217

  Wadhwa, Renu ^a,c   Duncan, Emma ^a   Kaul, Sunil C ^a,b   Reddel, Roger R ^a  

^a CHILDREN'S MEDICAL RESEARCH INSTITUTE   (Australia)

^b NATL INST BIOSCI HUM TECHNOL   (Japan)

^c CHUGAI PHARMACEUTICAL CO LTD   (Japan)

Author keywords

Differential display of mRNAs; Elimination of false positives; Modified reverse Northern

Indexed keywords

MESSENGER RNA;

ANIMAL; ARTICLE; CELL STRAIN 3T3; DNA PROBE; GENETICS; LABORATORY DIAGNOSIS; MOUSE; NORTHERN BLOTTING; STANDARD;

3T3 CELLS; ANIMALS; BLOTTING, NORTHERN; DNA PROBES; FALSE POSITIVE REACTIONS; MICE; RNA, MESSENGER;

EID: 1842564210     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02740775     Document Type: Article

References (8)

1. Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967-971.
2. Zhao, S., Ooi, S. L., and Pardee, A. B. (1995) New primer strategy improves precision of differential display. BioTechniques 18, 842-850.
3. 33P-labeled primer increases the sensitivity and specificity of mRNA differential display. BioTechniques 18, 424,425.
4. Reeves, S. A., Rubio, M-P., and Louis, D. N. (1995) General method for PCR amplification and direct sequencing of mRNA differential display products. BioTechniques 18, 18-20.
5. McClelland, M., Francoise, M-D., and Welsh, J. (1995) RNA fingerprinting and differential display using arbitrarily primed PCR. Trends Genetics 11, 242-246.
6. Callard, D., Lescure, B., and Mazzolini, L. (1994) A method for the elimination of false positives generated by the mRNA differential display technique. BioTechniques 16, 1096-1103.
7. Mou, L., Miller, H., Li, J., Wing, E., and Chalifour, L. (1994) Improvements to the differential display method for gene analysis. Biochem. Biophys. Res. Commun. 199, 564-569.
8. Chang, A. C.-M., Janosi, J., Hulsbeek, M., Jong, D. de, Jeffrey, K. J., Noble, J. R., and Reddel, R. R. (1995) A novel human cDNA highly homologous to the fish hormone stanniocalcin. Mol. Cell. Endocrinol. 112, 241-247.