Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways

Journal of Biotechnology

Volumn 109, Issue 1-2, 2004, Pages 159-167

  Ferreira, Bruno S ^a   Calado, Cecília R C ^a   Van Keulen, Frederik ^a   Fonseca, Luis P ^a   Cabral, Joaquim M S ^a   Da Fonseca, Maria M R ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Acetate; Heterologous cutinase; Physiology; Saccharomyces cerevisiae

Indexed keywords

BIOSYNTHESIS; METABOLISM; PH EFFECTS; PROTEINS; YEAST;

BIOSYNTHETIC PATHWAYS; RECOMBINANT PROTEINS;

BIOTECHNOLOGY;

ACETIC ACID; ACID; BASE; CUTINASE; GALACTOSE; RECOMBINANT PROTEIN;

BIOSYNTHESIS; CALCULATION; CONCENTRATION (PARAMETERS); CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME SYNTHESIS; FUNGAL STRAIN; FUSARIUM SOLANI; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; YEAST;

ACETIC ACID; CARBOXYLIC ESTER HYDROLASES; ETHANOL; FERMENTATION; FUSARIUM; GALACTOSE; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSE; HEXOSES; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

FUSARIUM; FUSARIUM SOLANI; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 1842530401     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2003.10.033     Document Type: Conference Paper

References (10)

1. Bitter, G.A., Egan, K.M., Koski, R.A., Jones, M.O., Elliott, S.G., Giffin, J.C., 1987. Expression and secretion vectors for yeast. In: Wu, R., Grossman, L. (Eds.), Methods in Enzymology, vol. 153. Academic Press, San Diego, pp. 516-544.
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
3. Carvalho C.M.L., Aires-Barros M.L., Cabral J.M.S. Cutinase: from molecular level to bioprocess. Biotechnol. Bioeng. 66:1999;17-34.
4. Giuseppin M.L.F., Almkerk J.W., Heistek J.C., Verrips C.T. Comparative study on the production of guar α-galactosidase by Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2 in continuous cultures. Appl. Environ. Microb. 59:1993;52-59.
5. Herwig C., von Stockar U. A small metabolic flux model to identify transient metabolic regulations in Saccharomyces cerevisiae. Bioprocess Biosyst. Eng. 24:2002;395-403.
6. Hitzeman, R.A., Chen, C.Y., Dowbenko, D.J., Renz, M.E., Liu, C., Pai, R., Simpson, N.J., Kohr, W.J., Singh, A., Chisholm, V., Hamilton, R., Chang, C.N., 1990. Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast. In: Goeddel, D.V. (Ed.), Methods in Enzymology, vol. 185. Academic Press, San Diego, pp. 421-440.
7. Mathews, C.K., van Holde, K.E., 1990. Biochemistry. Benjamin/Cummings, Redwood City.
8. Pampulha M.E., Loureiro-Dias M.C. Energetics of the effect of acetic acid on growth of Saccharomyces cerevisiae. FEMS Microbiol. Lett. 184:2000;69-72.
9. Stephanopoulos, G., Aristidou, A.A., Nielsen, J., 1998. Metabolic Engineering. Academic Press, San Diego.
10. van Gemeren I.A., Musters W., van den Hondel C.A.M.J.J., Verrips C.T. Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi. J. Biotechnol. 40:1995;155-162.