Characterisation of cysteine proteinases responsible for digestive proteolysis in guts of larval western corn rootworm (Diabrotica virgifera) by expression in the yeast Pichia pastoris

Insect Biochemistry and Molecular Biology

Volumn 34, Issue 4, 2004, Pages 305-320

  Bown, David P ^a   Wilkinson, Hillary S ^a   Jongsma, Maarten A ^b   Gatehouse, John A ^a  

^a DURHAM UNIVERSITY   (United Kingdom)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Cathepsin B; Cathepsin L; Diabrotica virgifera virgifera LeConte; Insect proteases; Protease inhibitors (PI); Yeast expression system

Indexed keywords

CATHEPSIN; CATHEPSIN B; CATHEPSIN L; CYSTEINE PROTEINASE; CYSTEINE PROTEINASE INHIBITOR; DIPEPTIDE; INSULIN; RECOMBINANT PROTEIN; TRYPSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BEETLE; CHEMISTRY; DIGESTIVE SYSTEM; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HYDROLYSIS; KINETICS; LARVA; METABOLISM; MOLECULAR GENETICS; PHYLOGENY; PICHIA; TISSUE DISTRIBUTION;

AMINO ACID SEQUENCE; ANIMALS; BEETLES; CATHEPSIN B; CATHEPSINS; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEINASE INHIBITORS; DIGESTIVE SYSTEM; DIPEPTIDES; ENZYME ACTIVATION; HYDROLYSIS; INSULIN; KINETICS; LARVA; MOLECULAR SEQUENCE DATA; PHYLOGENY; PICHIA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TISSUE DISTRIBUTION; TRYPSIN;

BOVINAE; DIABROTICA; DIABROTICA VIRGIFERA VIRGIFERA; INSECTA; PICHIA; PICHIA PASTORIS; ZEA MAYS;

EID: 1842487375     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ibmb.2003.11.005     Document Type: Article

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